Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,299	G→A	T1036T (ACG→ACA)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,299	0	G	A	93.6%	115.3 / NA	48	T1036T (ACG→ACA)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (18/26);  minor base C (2/1);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCC  >  NC_000913/3622085‑3622537                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               aCCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGCCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑GCCGGCTGACCACCATACCGAACGCCAGGAGCCGCCTCCAAACGAttttt                                                                                                                                                                                                            >  2:166448/1‑249 (MQ=11)         cATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCggg                                                                                                                                                                                                    <  1:166448/251‑1 (MQ=14)              cGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCt                                                                                                                                                                                               <  1:32677/251‑1 (MQ=14)                   gacagagccgtctgttGCATTACACGCTGACACAATATGAAGAGCCGCTTGTCGAAAGGCGCTATCTGTACGACCCGCGTGGCCGCAGTGTGGCAAAACGGTTGGGGCGGCGTGATCGGGACCTGACTGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACTGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCTGACGATTTATCAGCCGGGTAGCTTctc                                                                                                                                                                                           <  2:217060/235‑3 (MQ=11)                         aCCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGGGTGAACGGGACCCGACGGGCGGGGTGTCGCTGGCGCGGAAACCGCAAGTGACCTGGGACGGCTGGGGGGGCG‑AACCGCTGACCCCAATACAGAAAGACAGGGGCAGCATCCAGACGATTTATCAGCCGGGGAGcgtcccccccacc                                                                                                                                                                                    >  2:82119/1‑239 (MQ=11)                                ggTGCCCTACACGCGTACACAATATGAAGAGCCGCTTGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGGGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGATCGACAGGAGCCGCATCCAGGCGATTTATCAGCCGGGGAGCTTCATGCCACTCATCAgg                                                                                                                                                                              <  2:136182/250‑1 (MQ=11)                                 gTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGCAAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCCCTCATCCgggg                                                                                                                                                                            >  1:200544/1‑250 (MQ=9)                                           cgcgGACCCAATATGACGAGCCGCTGTGCGAAAGTCGCTATCTTTTCGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGTCTGGGTCGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGcc                                                                                                                                                                  <  2:231607/251‑1 (MQ=11)                                             cgGACACAATTTGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGGTGGGCCGCAGGGTGGCAAAAGGGGGGTGGCGGCGGGAACGGGACCTGACGGGCTGGAGGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATTCAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCAc                                                                                                                                                                <  2:68952/251‑1 (MQ=11)                                              ggacccaagaggAAGAGCCGCTGGTCGAAAGTCGCTATCTGTACGCCCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCAcc                                                                                                                                                               <  2:52549/240‑1 (MQ=255)                                                      tatGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACACTACAGAACGACCGGAGCCGCATCCAGACGCTTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCCCCGGTGAGCt                                                                                                                                                       >  1:115872/1‑251 (MQ=255)                                                         gAAGAGCCGCTGTTCGAAAGTCGCTACCTTTTCGACCCGCTGGGCGGCAGGGTGGCAAAACGGGTGTGGCGGCGGGAACGGGACCTGACGGTCTGTATGTCGCTGTCCCGGAACCCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCGCAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACTCCACTCATCAGGGTCGAAACTGCCACCGGTGTGCTGGc                                                                                                                                                    <  2:116800/251‑1 (MQ=11)                                                                 gCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGc                                                                                                                                            <  1:291879/251‑1 (MQ=37)                                                                           aGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGC‑ACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGc                                                                                                                                 <  1:181253/251‑1 (MQ=37)                                                                                     tttACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGAt                                                                                                                        >  1:122021/1‑251 (MQ=37)                                                                                       tACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGc                                                                                                                      >  1:173295/1‑251 (MQ=37)                                                                                          gACCCGCTGGGCCGAAGGGTGGCAAAACGGGCGTGGCGGCGTGAACGGGACCTGACGGGCTTGCTGCCGCTGTCCCGGCAACCGCCAGTGCCATGGTACGGCTGGGACTGCG‑ACCGGCTGCCCCCCATACAGAACGACAGGAGTCCCCTCCCCACGATTTATCACCCGGGACGCTTCACAACAATCATCAGGGTTGCAACTGCCACCGGTTAGCTGTCTAAAACGCCTCGCCGCAGCCTgtcagatcgccc                                                                                                                   >  2:34802/1‑240 (MQ=2)                                                                                           cccctgggggCCGCAGGGTGGCAACACGGGTGTGGCGGCTGGACCGGGACCTGACGGGCTGGATGGCGCTGGCACGGACACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCCGACCAAAATACAGATCGACAGGATCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCTCTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCTGATGCCCtt                                                                                                                  <  2:48816/244‑1 (MQ=11)                                                                                               gCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCTAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTcagc                                                                                                              <  2:145488/251‑1 (MQ=255)                                                                                                    gCCGCGGGGGGGCAAAACGGGTGTGGCGGCGGGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTcc                                                                                                         <  2:214689/251‑1 (MQ=255)                                                                                                      cGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGGGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCcgg                                                                                                       >  1:33522/1‑251 (MQ=255)                                                                                                       gCAGGGTGTCCAAACGGGTGGGGCGGCGGGAACGGGACCTGACGGGCTGGATGGCGCTGTCACGGAAACCGCAAGTGACCTTGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCcggc                                                                                                      <  2:758/251‑1 (MQ=14)                                                                                                            gTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCCGTCCGgcggcg                                                                                                  >  1:266379/1‑250 (MQ=255)                                                                                                              ggCAAAACGGGTGGGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTAGCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGaa                                                                                                <  1:176478/250‑1 (MQ=255)                                                                                                                  aaaCGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTAGCTGTCGCTGCCAAGGGAACCGCCAGTGACCTGCTACGGCTGGGAAGGCGCACCG‑CTGGCCCAGATCCAGAACGACAGGAGACGCAACCAGACGATTTATCAACCCGGGAGCTTCCCGCCACTCGTAAGGGTCGCAAATGCCCCCGGTGAGCTGGCGAAAACCCAGCGCCGCCACCTGGCGGGTGCCCTTTAACAGTCCGGCAGCGgagaagg                                                                                           >  2:313032/1‑248 (MQ=9)                                                                                                                     cGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCATGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTgg                                                                                        <  2:110723/251‑1 (MQ=255)                                                                                                                       ggTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGc                                                                                       >  1:120448/1‑250 (MQ=39)                                                                                                                                 cgTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGAGGTGtt                                                                            <  1:87793/251‑1 (MQ=255)                                                                                                                                  gTGAACGGGACCTGCCGGGCTGGATGTCGCTGTCGCGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTc                                                                           <  2:274017/251‑1 (MQ=255)                                                                                                                                       cGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCccgcc                                                                      >  1:125014/1‑251 (MQ=38)                                                                                                                                                  gggCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGAAGCAG‑ACCGGCTGACCCCAATACAGAACGACAGGTGCCGCATCCAGACGATTTATAAGCAGGGGAGCTTCACGCCAATCCTCCGGGTCGAAACTGCCACCGGTGCTCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGGGTGGTGTTCCCGCCGGAGCTGGTGc                                                           >  1:200476/1‑251 (MQ=14)                                                                                                                                                    gCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGt                                                                                                                                                            >  2:30431/1‑152 (MQ=255)                                                                                                                                                    gCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTGCACGCCACTCATCAGGGTCGAAACTGCCACCGGt                                                                                                                                                            <  1:30431/152‑1 (MQ=255)                                                                                                                                                       ggATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGGCGGCG‑ACCGGCTGAACACAATACAGAACGACAGGAGCCGCATCCAGACGCTTTATCAGCCGGGGAGCTTAACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGGAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCCGCAGTACGGCGGCGGAGACGGTGGCAGAGTGGTGTGAACGCCGGGGCAGGTGCAgctg                                                      >  2:59456/1‑248 (MQ=14)                                                                                                                                                       ggATGTCGCTGTCACGGAAACCGCAAGGGACCGGGTACGGCTGGGACGGCT‑ACCGGCTGACCACAATACAGACCGAAAGGAGCCGCACCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAATCTGCCACCGGTGAGCGGTCGAAACCGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGTGAAGAGGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGAt                                                       <  1:145180/250‑1 (MQ=14)                                                                                                                                                           gTCGCTGACACGGACACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑CCACGCCGGCCACAATACAGCACGACAGGACGCGCATCCAGACGATTTATAAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCACTTCAGCCGTCCGGCGGCGAAGACGGGGGCAGTGGGGGGGTCCCGGCGGTGCTGGCGCAGACGCTCg                                                  >  2:40680/1‑251 (MQ=11)                                                                                                                                                              gCTGTCAAGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCCGTGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCgccc                                               >  2:310605/1‑248 (MQ=255)                                                                                                                                                                    aCGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                         >  1:52780/1‑251 (MQ=38)                                                                                                                                                                         aaCCGCAAGTGCCCTGGTACGGCTGGGAGGGCG‑ACCGGCTGACCACAATACAGACCGACAGGAGCCGCACCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTCCCACCGGTGAGCTGGCGAAAACGCAGCCCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCTGCTgg                                         <  1:113505/246‑1 (MQ=14)                                                                                                                                                                         aaCCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGCTTTATCAGCCGGGGAGCTTCACGGCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCAGCAGCCTGGCGGATGCACTTCAGCAGTCCGGCGGCGAAGACGGGGGCGGGGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCCCGACCGGCTgggggtc                                    >  2:113505/1‑246 (MQ=11)                                                                                                                                                                              aaaGTGACTTGGTCGGGGTGGGACGGCG‑CCGGGCTACCCAAAATACAAACCGACGGGAGCCGCACCCGGACGATTTACCAGCCGGGGAGCTCCCCGCCCCTCATCAGGGTCGAAACTGCCACCGGTGGGCTGGCGAAACCGCAGCGCCGCAGCCGGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAAt                               <  2:120448/250‑1 (MQ=11)                                                                                                                                                                                  ggacggggaaCGGCTGGGACGGCG‑ACCGGCTGACCAAAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCGGGCGGATGCCCTTCAGCAGGCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTg                           <  2:266379/242‑1 (MQ=255)                                                                                                                                                                                          aaCGGCTGGGACGGCG‑ACCGGCCTACCACCATACAGACGGACAGGAGCCGCTTCCAGATGATTTATCACCGGGGGGGCCTCACCCCCCCCATCCGGGTGGAACCGCCCTCCGGTGAGCTGGCGAAAGCGCGGTGCCGCAGCCTGGCGGAGGCCCTTACGCCGGCCGGGGGCGAAGGCGGTGGCAGTGTGGGGTTCCCGCCGGTTCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCg                   <  2:193626/250‑1 (MQ=2)                                                                                                                                                                                                    aCGGCG‑ACCGGCTGACCACAATACAGAACGCCAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCCCGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATCCCCTTCAGCAGTCCGGCGGCTAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGtgagtgag         <  1:60050/251‑1 (MQ=255)                                                                                                                                                                                                     cGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACTATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAgg        <  1:28409/251‑1 (MQ=255)                                                                                                                                                                                                     cGGCG‑ACCGGCTGACCACAATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCTGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAgg        >  1:67394/1‑251 (MQ=255)                                                                                                                                                                                                            ccGGCTGACCACAATACAGAACGACAGGAGCCGCATCCCGACGATTTATCAGCCGGGGCGCTTCAAGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGAGTTCCCGCCGGTGCCGGGGCCGATTCTCGACCGGCTGGAAAGAGAAATCATGGCTGACCGGGTGCGCGAGGACAgcg  >  2:298433/1‑250 (MQ=14)                                                                                                                                                                                                            ccGGCTGACCAAAATAAAGAAGAACAGGAGCCGCATCCAGACGATTTACCAGCCGGGGGGCTTCCCGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGGGCCGCAGCCGGGCGGATGCCCTGCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgcc  <  1:311662/251‑1 (MQ=14)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCG‑ACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCC  >  NC_000913/3622085‑3622537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑