Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,443	C→G	intergenic (+577/+179)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,443	0	C	G	100.0%	139.9 / NA	48	intergenic (+577/+179)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/28);  total (20/28)

AAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCT  >  NC_000913/3560202‑3560679                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              aaaaTCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCatgat                                                                                                                                                                                                                                      <  1:144427/251‑1 (MQ=255) aaaaTCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCatgat                                                                                                                                                                                                                                      >  1:313738/1‑251 (MQ=255)  aaaTCTTCTTTTACCCTCAACGTATAAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGCTTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAGCTGGAAGGCAGCGCCGCAGAGCCatgatg                                                                                                                                                                                                                                     >  2:157807/1‑251 (MQ=255)           tttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGAt                                                                                                                                                                                                                            >  1:234937/1‑251 (MQ=255)               ccATCAACGTGTTAGAAACGTTGAGATTGTTGGTGACAATGCGCCAATTGCTGTGATTGAGCAGTGAGTGCGCTAACGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTACGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAgg                                                                                                                                                                                                                        >  2:118472/1‑251 (MQ=255)                cATCTACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGGGCGTGCGCTCCCGCTGCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCTTTGGGGAGTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGt                                                                                                                                                                                                                       <  2:133176/251‑1 (MQ=255)                         gtTAGCAACGTTGAGATTGTTGGTGACAAGGCGCAAATTGCGGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATGAACAGCGGCGAGCCATTGGGGATTTG‑CTCCGGCACTTTGCGGGCGATGCGCTCTTTTGCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGAGCAGGTGTTGCTcc                                                                                                                                                                                                               <  1:34251/250‑1 (MQ=255)                                  ggggatagtgggggtgcctaggcgcAAATTGCTGGGATTAAGCATGGCGTGCGCTACCGCTGCGGGC‑TGGGGCCGGATTGCGATAAACAGCGTGGTGCC‑TTGGGGGTGTGTCTCCTCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                       <  2:257128/228‑1 (MQ=255)                                     gagaTTCTCGGTGACAATGCGCAACTTGATGTGATTGAGCAGTGCGCGCGCTACCGCTTCCGGCGTGGAGACG‑ATATCGATACACAGAGTCGCGCCCTTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGATCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGTTTTTGCTCCGCCAGCTCATTgg                                                                                                                                                                                                  >  2:189232/1‑250 (MQ=255)                                            ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTAGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg                                                                                                                                                                                           >  1:306372/1‑251 (MQ=255)                                                           aaaTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCAG‑ATATCGATAAACAGCGTCGAGCCATTGGGGTTTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGTCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTAAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                            >  2:17321/1‑251 (MQ=255)                                                                 cggttaTTGAGGAGTGCGTGCGTTACCGCTTCCGGGGTGGTGCCG‑ATATTGAAAAACGGCGTCGAGCCTTTGGGTATTGT‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                      <  2:279209/246‑1 (MQ=255)                                                                  ggggtgggggcggtccgtgcgCGCCCGCTTCCGGCGGGGGGCCG‑ATATCGATAAAGAGCGTCGAGCAATGGGGGATTGG‑TGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                     <  2:232467/236‑1 (MQ=255)                                                                   ggtaTTGGGCAGTGCTGGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                     <  1:261002/247‑1 (MQ=255)                                                                                             cTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                           >  1:226215/1‑250 (MQ=255)                                                                                                  ggCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                              <  1:57250/194‑1 (MQ=255)                                                                                                  ggCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                              >  2:57250/1‑194 (MQ=255)                                                                                                   gCGTGGTGCCG‑ATATCGATAAACAGCGTCGCGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGTCATGATGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                                    <  1:53079/251‑1 (MQ=255)                                                                                                     gtggtgCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGTTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGTAAGGCAGCGCCGCAGCGCCATGATTGCTCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGTCTGCGGGTTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                                                                                                  <  1:189232/251‑1 (MQ=255)                                                                                                       ggtgCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                >  2:135831/1‑251 (MQ=255)                                                                                                         ggCCG‑ATATCGATAAACAGCGGCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                              <  1:124971/250‑1 (MQ=255)                                                                                                           ccG‑ATATCGATAATCAGCGTCGAGCCATTGTGGATTTG‑CGCCGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCGGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                                                                                                            <  2:313738/251‑1 (MQ=255)                                                                                                                  ccGATAAACAGCGCTGAGCATTTGGTGATTTG‑CTCCGCCGCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCTGACGGAGAAATGCTCTGCCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                      <  2:27049/250‑1 (MQ=255)                                                                                                                   caaTAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGTTACTGACATAACCCTGCTGTTTa                                                                                                                     <  1:239764/249‑1 (MQ=255)                                                                                                                          atgtgtcgcaccATTGGGGATTTG‑TTCCGCCACTTTGCGGGCGATGCTCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTATCCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGATAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                              <  2:230649/241‑1 (MQ=255)                                                                                                                             cGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGAGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTATACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCgg                                                                                                           >  1:276224/1‑250 (MQ=255)                                                                                                                                  ggCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                      <  1:135831/250‑1 (MQ=255)                                                                                                                                       ttGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata                                                                                                         <  1:182662/243‑1 (MQ=255)                                                                                                                                       ttGGGCATTTG‑CTCCGCCACTTTGCGGGCGATGAGCTATTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGTCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGATCGCGGCGAATAGTCTGCGGGCTGCCGGCGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTTCTGTTTAACCAGTTCGatactctcgg                                                                                                  >  2:182662/1‑243 (MQ=255)                                                                                                                                          gtgATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCTCCATGATGGCGCAGGTTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGTTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                              <  2:235426/249‑1 (MQ=255)                                                                                                                                            gATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGTCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAc                                                                                            <  1:40325/251‑1 (MQ=255)                                                                                                                                                       gCCAATTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAATGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg                                                                                  <  1:101989/251‑1 (MQ=255)                                                                                                                                                        ccACTTTGCGGTCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                 <  1:306791/251‑1 (MQ=255)                                                                                                                                                           cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTCCCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAACGTTGTGACGTTGTGTTTGTTTc                                                                              >  2:300573/1‑251 (MQ=255)                                                                                                                                                                    cGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                      >  1:217702/1‑202 (MQ=255)                                                                                                                                                                    cGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                      <  2:217702/202‑1 (MQ=255)                                                                                                                                                                      aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGTTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATTCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                                   <  1:204896/251‑1 (MQ=255)                                                                                                                                                                      aTGCGCTCTTTTTCTTCGGGCGGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATTCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                                   <  2:234937/251‑1 (MQ=255)                                                                                                                                                                          gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                               >  1:107523/1‑251 (MQ=255)                                                                                                                                                                                      cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttc                                                    <  1:142230/250‑1 (MQ=255)                                                                                                                                                                                        gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                 >  1:257350/1‑251 (MQ=255)                                                                                                                                                                                                        gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                               <  1:255560/141‑1 (MQ=255)                                                                                                                                                                                                        gATCGTCCCACGGCGTGTTACCCGCACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                               >  2:255560/1‑141 (MQ=255)                                                                                                                                                                                                                   ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                    <  1:60941/189‑1 (MQ=255)                                                                                                                                                                                                                   ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                    >  2:60941/1‑189 (MQ=255)                                                                                                                                                                                                                     cGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                    <  1:157807/251‑1 (MQ=255)                                                                                                                                                                                                                                       aGGCAGAGCCGCAGCGCCACGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCACCAAAAGCCATCGATAAACCCACgtct  >  2:281435/1‑248 (MQ=255)                                                                                                                                                                                                                                        ggCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt  >  1:308829/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              AAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCG‑ATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCT  >  NC_000913/3560202‑3560679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑