Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,539,535	G→A	L717L (CTG→CTA)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,539,535	0	G	A	100.0%	137.1 / NA	39	L717L (CTG→CTA)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/23);  total (16/23)

GCTCAGTTTGCCGATGGCGTCGAGACAATGAACGACCTGCAACCGGGTATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTG  >  NC_000913/3539290‑3539777                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   gCTCAGTTTGCCGATGGCGTCGAGACAATGAACGACCTGCAACCGGGTATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGTTGAAAGTGCTGGAAGTGGAGCGTCAGCGTAAACGTATCGCCCTAACtgt                                                                                                                                                                                                                                               <  2:154972/251‑3 (MQ=255)              aTGGCGTCGAGACAATGAACGACCTGCAACCGGGTATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGag                                                                                                                                                                                                                                  <  1:45401/251‑1 (MQ=255)                      gagaCAATGAACGACCTGCAACCGGGTATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTgg                                                                                                                                                                                                                          <  2:20881/251‑1 (MQ=255)                                             gggTATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCgcggcg                                                                                                                                                                                                   <  1:186655/251‑1 (MQ=255)                                             gggTATGATCATCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGCCGGCCTGGTTCACCTC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGCAAGCGGGCGCCATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCACCGCTCGTCgcggcg                                                                                                                                                                                                   >  2:98793/1‑251 (MQ=255)                                                tATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCgcggcggcg                                                                                                                                                                                                >  1:102965/1‑251 (MQ=255)                                                      ccTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGCAAGCGGGCGACATTGTGAAGGGGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGAGGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATg                                                                                                                                                                                          >  2:1771/1‑251 (MQ=255)                                                      ccTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATg                                                                                                                                                                                          <  1:10265/251‑1 (MQ=255)                                                             ggCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTTTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGGCGCGGCGGCGGTAATGAACGccc                                                                                                                                                                                   <  1:74720/251‑1 (MQ=255)                                                                        cAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAAcc                                                                                                                                                                        <  2:129427/251‑1 (MQ=255)                                                                              cACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCATGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCg                                                                                                                                                                   <  2:231842/250‑1 (MQ=255)                                                                                ccAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGGCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAGCGATGCGCCTGGAGGAGCAGCCTGGCGAAACCAACGCTTGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCa                                                                                                                                                                <  2:182735/251‑1 (MQ=255)                                                                                                 gTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTc                                                                                                                                                <  1:264333/250‑1 (MQ=255)                                                                                                 gTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTc                                                                                                                                                <  1:46107/250‑1 (MQ=255)                                                                                                    gATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCCTTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGCACGCCCGCAAAACAACCGCCCGGCCGCCCAACCACGCGGTCGTg                                                                                                                                             >  1:104172/1‑250 (MQ=255)                                                                                                     aaatgggcttGCACCAGGCCGGCCGGGTTCA‑ATCCTCTTCATGTTGGACCAAGTTTGTGGAAGATCCGCATACCTGGGTAAAGGCGGCCGACTTTTTGAAGGTGAAAGTGCTG‑AAATGGATCTTTAGCCTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAg                                                                                                                                          <  1:218006/242‑1 (MQ=255)                                                                                                            cGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGc                                                                                                                                    <  2:228563/251‑1 (MQ=255)                                                                                                               gCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACCCGGTCGTGAAGCGCAGCCtc                                                                                                                                 >  2:167524/1‑250 (MQ=255)                                                                                                                   cAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGCAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCgg                                                                                                                             >  2:42297/1‑251 (MQ=255)                                                                                                                    aGGACGGCCTGGTTCCCATC‑TCTTCATTGTCTGACAAGTTGGTGGAAGATCCGCATACCGTGGTGAAAGCGGTCGACATTGTGAAGGGGAAAGTGCTGTAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCGCGGCGCGGCGGCGGAAATGAACGCCCGCAAAACAACCGCCCGGCGGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGt                                                                                                                            <  2:279384/251‑1 (MQ=255)                                                                                                                         ggCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCCGCCGGTAATAg                                                                                                                       <  1:145602/251‑1 (MQ=255)                                                                                                                                                   cGAACAAGTTTGTGGAAGATCCGCATTCCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTAGGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCg                                                                                                                              <  2:12354/219‑1 (MQ=255)                                                                                                                                                   cGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCATAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCg                                                                                                                              >  1:12354/1‑219 (MQ=255)                                                                                                                                                    gAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcgg                                                                                             >  1:218356/1‑251 (MQ=255)                                                                                                                                                       cAAGTTTGTGGTAGATCCGCATACCGTGGTGCAAGCGGGCGACCTTGTGTAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGAAATGAAAGCCAGCACAACAACCGCCCGGCAGCCAACCAACGCGGTAGTAGCCCGCAGCCTGCAGGACATAGCGCGATGATGGATGCGGTtgcggcggga                                                                                           >  2:67830/1‑248 (MQ=255)                                                                                                                                                          gTTTGTAGAAGATCCGCATACCGTGGAGAAGGCGTGCGACATTGTGAAGGTGAAAGTGCGGGAAGTGGATCTTCAGCGTAACCGTATCGCCCGAACGAGGCGCCTGGATGAGCAGCCTGGCGAAGCCAACGCTCGTCGCGGCGCCGTTAATGAACGCCCGCAAAACAGCCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGAGGCGCGGGCGGCGGCAATgg                                                                                       <  2:276807/251‑1 (MQ=255)                                                                                                                                                              gtgGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCACTGGGCa                                                                                    >  1:162911/1‑250 (MQ=255)                                                                                                                                                                ggAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaa                                                                                 <  1:42297/251‑1 (MQ=255)                                                                                                                                                                 gAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaa                                                                                <  1:167524/251‑1 (MQ=255)                                                                                                                                                                    gATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGACAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAAAGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCCGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACg                                                                             >  2:313577/1‑251 (MQ=255)                                                                                                                                                                                           gggCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAACAGCCCGAACTGGCCTACg                                                      >  2:313612/1‑251 (MQ=255)                                                                                                                                                                                           gggCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACg                                                      <  1:227510/251‑1 (MQ=255)                                                                                                                                                                                                 cATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCg                                                 >  1:151242/1‑250 (MQ=255)                                                                                                                                                                                                                   gcgggAAGTGGATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCCGCCTGGCGAAACGAACGCTCGTCGCGGCGGCGGTAAGGAACGCCCGCAAAACACCCGCCCGGCAGCCAAACCTCGCGGTCGGGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGCACGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAACCGCCCGTACTGGCCTACGGGTCGAATTTGCACGAAAGCGTAg                              <  2:137503/248‑1 (MQ=255)                                                                                                                                                                                                                         aggggATCTTCAGCGTAAACGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAAGTTGCACGAAATCGTAGGGCAGa                        <  2:102965/248‑1 (MQ=255)                                                                                                                                                                                                                                   cAGCGTAAACGTATCGCACTAACTATGCGCCTGGCTGAGCAGCCTGGCGAAACCACCGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGACAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTACAAGCACGATGATGGATGCGCTGGCGGAGGCCATGGGCAACAAACGTTAAAAGACCGTACTGGCCTACGGTTCGAATTTGCACGAAATTGTaagtccaataaggcgtac              >  2:200756/1‑234 (MQ=255)                                                                                                                                                                                                                                      cGTAAACGTATAGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCAGCGGAGGAAATGACCGCCCGCAAAACAACCGCACGGCAGCAAAACCACGCGGTCGTGAAGCGCAACCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCACGTACTGGCCTACGGGTCGAATTTGCACGACATCGTAGGGCAGAGAAGGCGTTAAcg           >  2:177180/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaaCGTATCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCATCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAATCGCCCGTACTGGCCTACGGTGCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGGTCAcgcc         <  1:67830/250‑1 (MQ=255)                                                                                                                                                                                                                                               aTCGCCCTAACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAACACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCCAATAAGGCGTTCCCGCCGCATCTg  >  1:238884/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GCTCAGTTTGCCGATGGCGTCGAGACAATGAACGACCTGCAACCGGGTATGATCCTCGAAGGCGCAGTGACCAACGTCACCAACTTTGGCGCGTTTGTCGATATTGGCGTGCATCAGGACGGCCTGGTTCACATC‑TCTTCATTGTCGAACAAGTTTGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTG  >  NC_000913/3539290‑3539777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑