Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	154.3 / NA	47	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/27);  total (20/27)

CGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAG  >  NC_000913/3387829‑3388285                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGc                                                                                                                                                                                                                 <  1:204636/251‑1 (MQ=255)      aCGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGc                                                                                                                                                                                                            <  1:183515/251‑1 (MQ=255)         aGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCACCAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGtt                                                                                                                                                                                                         >  1:231319/1‑251 (MQ=255)                    cGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGACACAGCACACCATAAGCATCAAGGAGGGGGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCTCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGa                                                                                                                                                                                               <  2:157397/250‑1 (MQ=255)                        ccAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGa                                                                                                                                                                                          <  1:164833/251‑1 (MQ=255)                          aGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGaaa                                                                                                                                                                                        <  1:39305/251‑1 (MQ=255)                          aGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGaaa                                                                                                                                                                                        <  1:126656/251‑1 (MQ=255)                            cGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGt                                                                                                                                                                                                                               <  1:57617/210‑1 (MQ=255)                            cGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGt                                                                                                                                                                                                                               >  2:57617/1‑210 (MQ=255)                            cGAGGATATCCAGGGACAAAACCCGTCCCATATACAGCACACCAAAATCATCAATAATTGTGCGCGGATCATCGCAATGATGATCACAAGTCCGGCAATACAGCCAATAAATGTGCCGATGAGGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGc                                                                                                                                                                                      <  2:231319/251‑1 (MQ=255)                             gAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTg                                                                                                                                                                                           >  2:4349/1‑245 (MQ=255)                             gAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTg                                                                                                                                                                                           <  1:4349/245‑1 (MQ=255)                                             aaaaaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGt                                                                                                                                                                     <  1:158622/251‑1 (MQ=255)                                             aaaaaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGAGCGGATCATCGCAATGAGGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGACGGACCGGCGGCAACAATCGCCGCTGACAGTACCGCACAGCGTGGCGTTTACAGCTGGCAGTGAAAGCCAACAAACAGCGCCAgg                                                                                                                                                                     >  2:71243/1‑250 (MQ=255)                                                  ccGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAATACAAt                                                                                                                                                                <  1:91489/251‑1 (MQ=255)                                                      cccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACCGCGCCAGTACAATGGCg                                                                                                                                                            >  2:233554/1‑251 (MQ=255)                                                         aGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTc                                                                                                                                                         <  2:17944/251‑1 (MQ=255)                                                         aGATACAGCACAACAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATAGCCGCTGTCAGTACCGCCCAGCGGGGCGTTTCCAGCTGGAAGGGAAAGCCAAAAAACAGCGCCAGTACAATGGCggcc                                                                                                                                                         >  2:177742/1‑249 (MQ=255)                                                             aCAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCa                                                                                                                                                     <  1:234526/251‑1 (MQ=255)                                                                        gAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGc                                                                                                                                           >  1:241344/1‑250 (MQ=255)                                                                           acatcaATAATGGTGCTCGGATCATCGCAATGATGAACACCAGTCCGGCAAGACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAg                                                                                                                                       <  2:71240/250‑1 (MQ=255)                                                                               caATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGaa                                                                                                                                   <  1:43717/251‑1 (MQ=255)                                                                                 ataatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCACTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGAGCCAGTACAATGGCGGTCGCCAGTTTTACCGCCAAGCGAat                                                                                                                                  >  1:311852/1‑250 (MQ=255)                                                                                                             gatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTAcc                                                                                                                                             <  1:27047/211‑1 (MQ=255)                                                                                                               tCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTTCCGCAAAGCGCAAATGGTGGTTAGCCATGGAGGAAATACCCaac                                                                                                   >  2:35930/1‑249 (MQ=255)                                                                                                                cACCAGTCCGTCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCCCCTCCCGCAGCAAAGGCCGGACCGGCGGAAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCGGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAGGTTGTTTAGCAATGGAGAAAATACCCATCg                                                                                                  <  2:59611/251‑1 (MQ=255)                                                                                                                  ccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTg                                                                                                <  1:103575/251‑1 (MQ=255)                                                                                                                            aaTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCaaa                                                                                     <  1:112666/251‑1 (MQ=255)                                                                                                                                 aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCACGCAGCAAAGGCCGGACCGGCGGCAACAATCCCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCCAAGCGAATATGTTGGTTAGCAATGGGAAAAATACCCATCGTG‑ATATACCaaaattcc                                                                                >  1:10168/1‑246 (MQ=255)                                                                                                                                                cGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTTACGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCAACGGG‑GTTAACCAAACTCACGAAGGAGATGGGcc                                                                  >  1:54895/1‑250 (MQ=255)                                                                                                                                                 gatgatGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTAcc                                                                                                                                             <  2:216995/175‑1 (MQ=255)                                                                                                                                                 gatgatGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGGTAGCAATGGAGAAAATACCCATCGGG‑ATTAACCAAACTAACGCAGGCGATGGGCCAt                                                                >  1:230293/1‑251 (MQ=255)                                                                                                                                                 gatgatGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCCGTTTTAcc                                                                                                                                             >  1:216995/1‑175 (MQ=255)                                                                                                                                                              aaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAAc                                                   <  1:35930/251‑1 (MQ=255)                                                                                                                                                                  cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGGATATGTTTGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGaa                                                >  1:64184/1‑250 (MQ=255)                                                                                                                                                                  cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAAt                                               <  2:64184/251‑1 (MQ=255)                                                                                                                                                                         gCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGGGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGGTGGGCCATTTTACGGAAGAACGAATCCTGGCt                                        >  1:224022/1‑251 (MQ=255)                                                                                                                                                                            aaTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAGCGAATATGTTGGGTAGCAATGGGGAAAATACCCATCGTG‑ATTAAACAAACTCACGCAGGCGATGGGCCATTTTACGGGAGAAccgatccaggcgttc                                     >  1:164634/1‑237 (MQ=255)                                                                                                                                                                               aGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtc                                  <  1:129830/251‑1 (MQ=255)                                                                                                                                                                                  gcCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTAACGCAAAACGAATATGTTGGTTAGCAATTGAGAAAATAACCATCGTG‑AGTAAACAAACTCAAGCAGGCGAGGGGCCAATTTACGGGAGAACGAAACCCGGCTTTCGTcgcg                               >  2:169492/1‑251 (MQ=255)                                                                                                                                                                                          acgttccACCTCCCGCAGCAAATGCCGGACTGTCGGCAACATGCGTCGTTGTCAGTACCGCCCATCGTGGCATTTCCAGCAGGAAGAGAAAGCCAACAAACGGCTCCAGGACAATGGCGATCGCCAGTTTTACCGCAAAGCGAATATGTTGGTGAGCAATGGAGAAAATACCCTTCGTG‑ATTAACCAAACTCTCTCAGGCTAGGTGTCATTGTACTGGAGTTCGGATCCTGTCTTCCGTCGCGATCTTGtt                       <  2:8863/245‑1 (MQ=255)                                                                                                                                                                                            ggTTCACCTCCCGCAGCAAAGGCAGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGAGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTAGACAGTTTTTCCGCAAAGCGAATATGTTGGTTAGCAATGGGGAAAATACCCATCGCG‑ATTAACCAAACTCACGCAGGAGGTGGGCCCTTTTACGGAAGAAAGAAACCTGGCTTTCTTCGCGATTTTGtttt                     >  2:312329/1‑250 (MQ=255)                                                                                                                                                                                             gTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAGCGAATATGTTGGGTAGCAATGGAGAAAATAACCATCGGG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAAAGAATCCTGGCTTTCGTCGCGATCTTGTTTGc                    >  1:13898/1‑251 (MQ=255)                                                                                                                                                                                                cACCCCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGGCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCa                  <  2:10168/250‑1 (MQ=255)                                                                                                                                                                                                        gcagcaAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTGGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGaccacc         <  1:34304/251‑1 (MQ=255)                                                                                                                                                                                                              aaGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGGTGGGCCCTTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGtt   >  1:168967/1‑250 (MQ=255)                                                                                                                                                                                                               aGGCCGGACCGGCTGCACCAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTCAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCATTTATACCGCAAAGCGAATATGTTGGTTAGCAAGTGAGAAAATTCCCATC‑TGTAGTAACCAATCTCGCGCGGGCTATGGTCCATTTGACGGAAGAACGAATCCTGGCTTTCGTCGCGATGTTGGTTGCCAGTGACCTCCGCTGTAg  <  2:204852/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAG  >  NC_000913/3387829‑3388285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑