Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,159	A→C	C303G (TGT→GGT)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,159	0	A	C	100.0%	35.6 / NA	14	C303G (TGT→GGT)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/2);  total (12/2)

CGAGGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGAT  >  NC_000913/3265934‑3266396                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              cGAGGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTATCGCTTAAATAATGTCCTCAATAGCAACCGGCAGATCGTCTGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTg                                                                                                                                                                                                                      >  1:45223/1‑251 (MQ=255)                gtattaaTTCGCCCAGCCAGTCTTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCTGATCGTATTTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACATGTGGATTATTTATGTTTAAATAATAACAAATAACTAACCAACTTCTATTAATTGCCGTCGTCTACCCCCATTATAAGCTGAATCCTCTTTGGCTAATTCCACCaa                                                                                                                                                                                                        <  2:899/245‑1 (MQ=255)                                        tgttTCGATTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGAAACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACg                                                                                                                                                                                >  2:16538/1‑250 (MQ=255)                                                  cAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATCCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCg                                                                                                                                                                     >  1:149229/1‑251 (MQ=255)                                                      aatGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGAcc                                                                                                                                                                  >  1:296810/1‑250 (MQ=255)                                                                                      tAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGttt                                                                                                                                 >  1:10985/1‑251 (MQ=255)                                                                                                                            tGTAGGCAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGATTAAATAACCACAAAAAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTATGGCTAATTCCACCAAAACCGCTACTGCTTTTTTAATAAGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATCGTAATAAATTGATTAGAACaaaa                                                                                            >  2:61186/1‑250 (MQ=255)                                                                                                                                ggTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAA‑TAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTg                                                                                       >  1:96495/1‑250 (MQ=255)                                                                                                                                                                       tatgtatgttcacaaaacaaCACATAACTAACCAACTTCTCTTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCCCCAACACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGAATATTGTGCCACATGTAATGTTTCTTCAACCGGCATAGTAATAAATTGATTAGAACCAAAAGGTGCCGTCATATCCCAAGGAATTACAGTTAAGACATCAGCAt                                                >  2:101115/19‑251 (MQ=255)                                                                                                                                                                                 taaataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCAATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTGTCGACCATACCGAGGCATATTGTGCCACAGGTAATGTTTCTTCAACAGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACa                                        >  1:316698/1‑249 (MQ=255)                                                                                                                                                                                         aaCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATaa                               >  1:292342/1‑250 (MQ=255)                                                                                                                                                                                               taactaacCACCTTATATTAATTGCCTTCGACTACCCCCCTTACAAGATGAATACTCTTTGGATAATTCAACCAACACCGATGATGCTTTCTTAATACGATAATTTTTCGACCATACCGCGGAATATTGTGCCAAAGGTAATGTTTCTTCAACCGGAATAGTATTAAATTGATTAGAACCAAAAGGTGACGTCATATAACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAACTTGTc                         >  2:129682/1‑250 (MQ=255)                                                                                                                                                                                                        aaCTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGt               >  2:237001/1‑251 (MQ=255)                                                                                                                                                                                                                      gCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGAt  <  2:296810/250‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              CGAGGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGAT  >  NC_000913/3265934‑3266396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑