| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,221,763 | C→T | L100F (CTC→TTC) | ebgR → | DNA‑binding transcriptional repressor EbgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,221,763 | 0 | C | T | 96.3% | 81.7 / NA | 27 | L100F (CTC→TTC) | ebgR | DNA‑binding transcriptional repressor EbgR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/12); minor base A (1/0); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATCCAGGGTCTTAAATGACGATCCGACATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGG > NC_000913/3221517‑3222007 | gTATCCAGGGTCTTAAATGACGATCCGACATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCAc > 1:188556/1‑251 (MQ=255) aTCCAGGGGCTTAAAAGTCGATCCGACATTGAATGTGAAAGAAGAGACGAAACAACGCAGTCTCGAGGGCGCCGAAAAGCTGGAGTACAAGACCATTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCGAGCTAGATCTATCAGCAAGACCGGGAGATCAACGAGCCTTACTATCGTGCGATCCGCCACGGCATGGAAACCCGGTGCGAATATCTGGGCATCTAGTTCAcc < 2:253784/250‑1 (MQ=255) tCCAGGGTCTTAAATGACGATCCGACATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCa > 1:241523/1‑250 (MQ=255) gATCCGACATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTAGCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCgg < 2:262451/251‑1 (MQ=255) cGACATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCtt > 2:172415/1‑250 (MQ=255) gAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGAc > 1:47315/1‑250 (MQ=255) aaaGAAGAGACGAAACATCGCAATCTCGAGATCGCCGAAACGCTGGAGCAAAAGACCAGTAGTGCACGTACACTCCAGACAGGTGCAGACAACCAAAACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAAAGATCCTTACTATCTGGAGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACAAACTGTTATGAACACAGCGGCTTACCAGACATTaaaa > 1:97645/1‑250 (MQ=255) aagaGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTc > 1:77771/1‑251 (MQ=255) aTCGCCGAAAAGCAGGAGTACAAGACCAGCAGGTCCCGTAAACTCCAGACAGGCGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGCCATGAAAAACGTCACCGGTATTTTAATTGTCGGCAAAcc < 2:239514/251‑1 (MQ=255) aaaaGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGATGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGATCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGCCACCGGTATTTTAATTGTCGGCAAACCCAcgccc > 1:274499/1‑251 (MQ=255) aGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCAcgcccgcc > 2:75048/1‑251 (MQ=255) ggAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACCCCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTCCTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTg < 2:272696/250‑1 (MQ=255) gACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGTCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTg < 2:241523/251‑1 (MQ=255) aaCTCCAGACAGGTGCAGTCACCCAACACCATCTTCTGGCTATCTACAGCTCCCAGCAGGAGCTGGAGATCCACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCAcc > 2:307058/1‑188 (MQ=255) aaCTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATGGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCAcc < 1:307058/188‑1 (MQ=255) tCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGAc > 1:222286/1‑251 (MQ=255) aCGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATc < 1:107202/251‑1 (MQ=255) gATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAg < 1:75048/251‑1 (MQ=255) ttACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGATATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACACTATCTGTTTTATCGACTTTCACGAACCCGGCCGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTaaa > 1:282281/1‑250 (MQ=255) aCTATCTGGCGATCCGCACCGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAGCCGTTATGAACACCGCGTCTTCCCAGACATTAAAGACGTCACCGGTATTTTAATTGTCTGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCTCGTTGACCGACAATATCTGTTTTATCTACTTTCACTAACCCGGCAGCGGTTACGATGCGGTGGATATCGAGCTGGCACGCATCAGTAAAGa < 2:115876/250‑1 (MQ=255) aTCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAatc > 1:95506/1‑251 (MQ=255) tGAAACCCAGTGCGAAAAGATGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGAGGTGGATAACGATCTGGACCGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAgg > 2:2427/1‑251 (MQ=255) gAAACCCAGTGAGAAAACCTGGGCATCGAGTTCACCACCTGTTATGAACACTGAGGCTTACCAGACATTCAAACCGTCACCAGTATTTTAATTGTCGGCAAACCCACGCCCGCACTGCGCGCCGCTGCCAGCGCGTTGACCGACACTATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGCGGATATCGATCTGGCACGCCTCAGTAAAGAAATCATCGACTTCTATATCCACCAg > 2:287956/1‑249 (MQ=255) aCCCAGTGCGAAAAGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGt > 1:223880/1‑251 (MQ=255) aaaGCTGGGCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTg < 1:172415/251‑1 (MQ=255) gCATCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGATCCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATAACAACCAGGGCGTTAATCGTATTGGTTTTAtt < 2:249900/251‑1 (MQ=255) aTCGAGTTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTgg < 1:153348/251‑1 (MQ=255) | GTATCCAGGGTCTTAAATGACGATCCGACATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGTGCCCGTAAACTCCAGACAGGTGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGG > NC_000913/3221517‑3222007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |