| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,153,055 | G→A | A274T (GCT→ACT) | metC → | cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,153,055 | 0 | G | A | 93.3% | 38.8 / ‑3.3 | 15 | A274T (GCT→ACT) | metC | cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (7/7); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
AAATATCTGGTTGGGCATTCAGATGCGATGATTGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAAC > NC_000913/3152863‑3153289 | aaaTATCTGGTTGGGCATTCAGATGGGAGGTTGGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTgg < 1:202740/251‑1 (MQ=255)aaaTATCTGGTTGGGCATTCAGATGCGATGATTGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTgg > 1:191912/1‑251 (MQ=255) tGGGCATTCAGATGCGATGATTGGCACTGCCGTGTGCAATGCCCGTTGCTGGGCGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATCTAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTc > 1:19833/1‑251 (MQ=255) tGGGCATTCAGATGCGATGATTGACACTGCCGTGTGCACTGCCCGTTGCTGGGAGAAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGAGAGCGTTTGCGTCACCATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGtt > 2:194512/1‑211 (MQ=255) tGGGCATTCAGATGCGATGATGGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCAGGAGGGTCGTTGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGtt < 1:194512/211‑1 (MQ=255) aTGCGATGATTGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAAGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTgg > 1:103638/1‑251 (MQ=255) aggatTGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCTATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACg < 1:137720/249‑1 (MQ=255) cGTGTGCAAGTCCCGTTGCTGGGAGCAGCTACGGGAAAAGGCCTATCTGATGGGCCAGATGGGCGATTCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGgca < 1:34150/251‑1 (MQ=255) gCAATGCCCGTTGCTGGCAGCAGCTACGGGAAAATTCTTATCTGTTGGGCCCGTTGGACGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGGTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATTGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCTCGAATTCTGTAAACCAGACTTTACAGGAagcagc < 2:248206/251‑1 (MQ=255) tGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGGCGAAc > 1:152009/1‑251 (MQ=255) aGCCGTGGCCTGCGCACATTAGGTGTCCGTGTGCGTCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTTCCTGGCAGTAAAGGTCTCGAATTCTGGAACCGAGACTTTACAGGCAGCATCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGTCGAACTATCTGGATAACTTCAGTTTATTCAGCAt < 1:100836/251‑1 (MQ=255) cgcACATTAAGTGTGCGTTTGCGGCAACATCATGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGTTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAATACTCAATTATGAAGAGCTGTCGAACTATCTGGATAACTTCAGTTTATTCATCATGGCCTACTCGTg < 1:44286/251‑1 (MQ=255) atcatgtcaGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCaataa > 1:90585/9‑166 (MQ=255) atcatGAAAGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTTCTCTGCCTGGCAGTAAAGTTCACTAATTCTGGAAACGAGACTTGACAGGCAGCAGCGGGCTATATTCCTTTGTGCTTAAGAAAAAACTCaataa < 2:90585/166‑1 (MQ=255) aaGCAGTCTGAAAGTGACTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGACTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAAc > 1:285523/1‑251 (MQ=255) | AAATATCTGGTTGGGCATTCAGATGCGATGATTGGCACTGCCGTGTGCAATGCCCGTTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAAC > NC_000913/3152863‑3153289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |