Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,602,552	0	C	T	65.4%	17.0 / 19.5	26	E103K (GAG→AAG)	yjjP	putative succinate exporter YjjP
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base T (6/11);  total (9/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTG  >  NC_000913/4602310‑4602795                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aaGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCtt                                                                                                                                                                                                                                             <  1:207841/251‑1 (MQ=255)  aGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                                                                                                                                                                                                                            <  1:186906/251‑1 (MQ=255)        ccGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAAt                                                                                                                                                                                                                                       <  2:2849/250‑1 (MQ=255)         cGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAACAGTTGCTCTGTGGATGAAGATGACGTTGTGACAGCAGCTGGCGGAAATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCCGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGATTCTTTACGCCTTTGTAATcc                                                                                                                                                                                                                                     >  1:290700/1‑251 (MQ=255)                      ggcggcGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGcc                                                                                                                                                                                                                        >  2:69075/1‑250 (MQ=255)                        gggcgAAAGCGGTAAGGCAAAAGTTGAGCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCGTTACGCCTTTGTAATCCAGCAGATGATGCTcc                                                                                                                                                                                                                      <  2:187291/250‑1 (MQ=255)                                          aaaaGTTTGTCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGAGGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCAGCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATTATGCTCCGCAAGAATCACAATGTGc                                                                                                                                                                                                    <  2:61639/251‑1 (MQ=255)                                                      gtgGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCATCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCGTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACtt                                                                                                                                                                                             <  1:154587/246‑1 (MQ=255)                                                                    cGTTGTGCCAGCAGCTGGCGGATATACAGCGCGGTCGGACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTAGTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTTGCTAAAGCGTTTCTTTACGCGTTGGTAATCCAGCAGATGATGCTGCTCAAGAATCACAATGTGCGGGACTGCCGGCACCAGATTCATAtt                                                                                                                                                                          <  2:290700/251‑1 (MQ=255)                                                                          gCCAGCAGCTGGCGGATATACATCGCGGTCGTTCTTGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGTGTAACGTAATGGCTGAATTTGGCGAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGTCTTCAGTCACCACATTCATATTAATGc                                                                                                                                                                     <  2:189786/250‑1 (MQ=255)                                                                             agcagcTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCcgcg                                                                                                                                                                 <  1:33077/251‑1 (MQ=255)                                                                                      cGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAAAAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGACATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGCTCGTGAtt                                                                                                                                                        >  2:211228/1‑251 (MQ=255)                                                                                                                                   ccagcccAGCCACCGTTATTGAGTGTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCAGCTTGGGTAACGTAATGGCTTAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAAGCCAGCAGTTGATGCTCCGCAATAATCACAATTTGCTGGACTTCAGTAACCACATTCATATTAATGCCTCGATAGTGATTTTTACTTGCCGATGTCAGGCATTGCCCATCTTTAATAGGTGTCAg                                                                                                           <  2:178703/247‑1 (MQ=255)                                                                                                                                                        aGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCGTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGAt                                                                                      <  1:123130/251‑1 (MQ=255)                                                                                                                                                                 aaaCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGGCGATGTCAGGCATTGCCCATCTTTAATAGTTGGCAGCACTATGGCGTTCGAAGAGATAGAACTTTc                                                                             <  1:287048/251‑1 (MQ=255)                                                                                                                                                                           aaGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTcc                                                                   <  1:296635/251‑1 (MQ=255)                                                                                                                                                                               aaGGCCAACCATTAAGGCTACCAGCCATATTGGGTAACGTAATGGCTGAATTTGGATAAATCGTTTCTCTACGCCTTTGTAATCAAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCCTATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCACATCTTTAATAGTTGTCAGCACGATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTc                                                               >  2:115911/1‑251 (MQ=255)                                                                                                                                                                                              ggCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGAccc                                                 >  1:188056/1‑250 (MQ=255)                                                                                                                                                                                                        cATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATg                                                                                                                                                                      <  2:86030/123‑1 (MQ=255)                                                                                                                                                                                                        cATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATg                                                                                                                                                                      >  1:86030/1‑123 (MQ=255)                                                                                                                                                                                                                         tGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAAcg                     >  2:209927/1‑251 (MQ=255)                                                                                                                                                                                                                          ggCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAAcgc                    <  1:69075/251‑1 (MQ=255)                                                                                                                                                                                                                             tGAATTTGGCTAACTAGATTCTTTACGACTTTGAAATCCAGCCGATGATGCTCCGCAAGAATCACAATGTGATGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTACCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAAcgcgat                 >  2:204252/1‑249 (MQ=255)                                                                                                                                                                                                                             tGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGCTCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCt                 >  1:28934/1‑251 (MQ=255)                                                                                                                                                                                                                                            cGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg  <  1:55871/251‑1 (MQ=255)                                                                                                                                                                                                                                            cGTTTCTTTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTGCGAATAGATAGAACTTTCGACGCTGTCCATTCCCAGGGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGtt   <  1:19190/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    AAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTG  >  NC_000913/4602310‑4602795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑