Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,905,2191.C40.0% 5.6 / 21.7 15coding (200/672 nt)queEputative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base . (5/4);  new base C (2/4);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGCTTTGGCGATACGGTAACCCAGGTATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAG  >  NC_000913/2905001‑2905457
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
tCAGCTTTGGTGATACGGTACCCCAGGTATTCGGTGTGCAGCGTACCTCATGAGTACCCCTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGTAAATCATGAATGCAAGGCTCACCACCCGTAAGCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCGTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCGTCTCCAGAAt                                                                                                                                                                                                                 <  2:185795/250‑1 (MQ=255)
                        aGGTATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATc                                                                                                                                                                                         >  1:166673/1‑251 (MQ=255)
                                            aCCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCTTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGGTTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtg                                                                                                                                                                      <  2:238649/250‑1 (MQ=255)
                                            aCCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgt                                                                                                                                                                     <  1:83870/251‑1 (MQ=255)
                                                cATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGtt                                                                                                                                                                  <  1:307510/249‑1 (MQ=255)
                                                  tGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCGGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgt                                                                                                                                                              >  1:106700/1‑251 (MQ=255)
                                                                       tGGCAGCTAACACCGCTCTTTTCGAGTCGATCAGTAAGAGGCCGCCCATCATGAATGCAAGCCTCCAAACCCGTAATCAACACATGACGCGCGGTGTATCCAAGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAACCCCCCC‑ACTTATCACCCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGCGTGTTTGGTGTCGCACCAGGCACAGCCa                                                                                                                                           >  2:284324/1‑250 (MQ=255)
                                                                          cAGCTAACACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGACTGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCg                                                                                                                                       >  2:160639/1‑251 (MQ=255)
                                                                                             cGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAAt                                                                                                                    <  1:160639/251‑1 (MQ=255)
                                                                                                           agtGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAAc                                                                                                     <  1:76966/251‑1 (MQ=255)
                                                                                                                        cATGAATGCAAGGCGCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCCCACTTAGCACTCTCTTTGGTCTTCGCCTGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGGTTTTCCCAGGTGTGTTTGGTGGCGCACCAGGCACAGTCAACCGGGCATTGCGGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTa                                                                                         <  1:18083/251‑1 (MQ=255)
                                                                                                                          tGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGGAAAgaaaac                                                                                      >  2:276810/1‑246 (MQ=255)
                                                                                                                                                      ccacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAAAGCCGGGAACATAACCCTCCCCTTGCAGGGTCTGGAACAtcccc                                                          >  2:13533/1‑248 (MQ=255)
                                                                                                                                                                                                  aaaTCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAACAGGGCGACTTACCGATCCTc                                                                                                                                                                                      >  2:289833/1‑83 (MQ=255)
                                                                                                                                                                                                                cGCA‑GCCCCCC‑ACTTAGCACCCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCATGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTGAATTATTGCAg  <  2:106700/249‑1 (MQ=255)
                                                                                                                                                                                                                           c‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGa                           >  1:4629/1‑214 (MQ=255)
                                                                                                                                                                                                                           c‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGa                           <  2:4629/214‑1 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
TCAGCTTTGGCGATACGGTAACCCAGGTATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCA‑GCCCCCC‑ACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAG  >  NC_000913/2905001‑2905457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑