Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,778	0	C	T	41.7%	5.8 / 22.3	24	A379T (GCC→ACC)	rnb	RNase II
Reads supporting (aligned to +/- strand):  ref base C (6/8);  new base T (2/8);  total (8/16)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATT  >  NC_000913/1347555‑1348024                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       cgcggtttgcgtaaaCCGTCCAGCGTGAGCACTTCTTCGGTATCGACATGCAGACCGGGCGTTTTCAGCAGCGCTGCCAGCGCGTCGGCATTCGTCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTGCGCGGGCCGCACAAAGGTTAGCGGCAATCATCGCTTCTTCGACTATACGGTTGGCGATGCGACGTGGTTCGGCGACGATATCCATCACTGCACCTGTTTc                                                                                                                                                                                                                             <  2:193269/239‑1 (MQ=255)         gCAGAAACCGGCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGTCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGaa                                                                                                                                                                                                                     <  1:305629/251‑1 (MQ=255)                    ccAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTa                                                                                                                                                                                                           <  1:214298/250‑1 (MQ=255)                     cAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCTCGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCGTCTTCGACGATACGTTTGGCAATGCGACGAGGCTCAGCTGCGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                                                                                                                         <  1:95405/251‑1 (MQ=255)                            aGCACTCCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCg                                                                                                                                                                                                  >  1:84381/1‑251 (MQ=255)                                        gCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCGTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCTGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAacac                                                                                                                                                                                      <  1:274766/251‑1 (MQ=255)                                            cGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGt                                                                                                                                                                                  <  1:236919/251‑1 (MQ=255)                                                                                         cATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTCGCGGCAATCATCGCTTCTTCGCCGATACGGTTGGCAATGCGACGAGGCTCGGCAACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTGTGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCt                                                                                                                                     >  1:252080/1‑251 (MQ=255)                                                                                         cATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCt                                                                                                                                     >  1:223548/1‑251 (MQ=255)                                                                                                   accAAAGCTCATATGCACGTTATAGATGCCAAGACCGAGCTCATCGCGCAGTACGTGGGCCGCACAAATGTTAGCGACAATCATCGCTTCTTCGATGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTGACCTTTTACACCGAGATTAAAGCGGTAACCCGGGCCGTCGTGAAACACCAGGGCGTTGTTATGACTCCACTCGCCGCGGCGGTGGCAAATTTGCGCTAGCAAACGGa                                                                                                                           <  1:129863/249‑1 (MQ=255)                                                                                                       aaGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTg                                                                                                                       <  1:202063/251‑1 (MQ=255)                                                                                                              tatGCCCGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACACGTGCCGCAAACATGTTAGCGGCAATCATCGCTTCTTCGACGATCCGGTTGGCAATGCGACGAGGCTCGGCGCAGATATCCAGCACTTCACCTTTTTCACCGAGAATCAAGCGGGAATCCGGGCGCTCTTTTAACACCAGTGAGTGGTTCTGACGCCACTCGCACCGGCGTTTGCAATTTTGCGCTAGCCAACGGACTTGTTCGGc                                                                                                                 >  2:158633/1‑250 (MQ=255)                                                                                                                 tcACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGGTCGCGGGCCGCACATATGTTAGCGGCAATCATCGATTCTTCGACGGTACGGTTGGTAATGCGACGAGGCTCGTTGACTATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCTCTCGCCGCGGCGTTGGCAAATTTTCGCTAGCAAACGGACTTGTTCGGCaa                                                                                                               <  2:51192/248‑1 (MQ=255)                                                                                                                       tataGATGCCAAAACCGAGATTATCGCGCAGTACGCGGGCCGCACAACTGTTAGCCGCACTCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCCCTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTcc                                                                                                       >  2:39069/1‑250 (MQ=255)                                                                                                                            aTGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTc                                                                                                  <  1:270515/251‑1 (MQ=255)                                                                                                                                       gAGCTTATCACACAGTACGCGGGCCTCCCAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGAACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCTGGCGATCTTTAAACACCAGTGCGTGGTTATGACGACCCTCGCCGCGGCGTTGGAAAATTTGCTATAGAAAACGGACTTGTTCGGCCATTTCTTCCTTTTAAGGATGCCAGt                                                                                       >  2:288221/1‑251 (MQ=255)                                                                                                                                                         gcgGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTccagcc                                                                     <  2:5299/251‑1 (MQ=255)                                                                                                                                                                gCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGCAATCCGGGCGATCTTTGAACACCAGTGc                                                                                                                                                                                >  1:58344/1‑137 (MQ=255)                                                                                                                                                                gCACAAATGTTAGCGGCAATCATAGCTTCTTCGACGATACGGTTGGCAAGGCGACGAGGCTCTGCGACGATATCGAGCACTTCACCTTTGTCACCGAGAATAAAGCGGTAATCCGGGCGATCGTTAAACACCAGTGc                                                                                                                                                                                <  2:58344/137‑1 (MQ=255)                                                                                                                                                                                              tcGACGAGTCGGTTGGCATTCCGCCGAGGCTCGGTGACGAAATCCAGCACTTCGCCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAgctttgcttt                                   <  2:235002/248‑1 (MQ=255)                                                                                                                                                                                                      aCGGTTGGCAATGCGACGAGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGAtg                         <  2:223548/250‑1 (MQ=255)                                                                                                                                                                                                              cAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACATTTTTCACCGAGGATAAGGCGGTAATCCGGGCGATCTTTAAACAACAGTGCGTGGTTATGACGCACCTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAAAGGCCTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTCTTTTCCAGCCAGTAAGAAACCTAGTCATACACCAGCTTTGCTTTGGTTTCGATGGTggcggc                 >  2:269059/1‑250 (MQ=255)                                                                                                                                                                                                                     acgaGGCTCGGTGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAAtt         <  1:119037/251‑1 (MQ=255)                                                                                                                                                                                                                            gcGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTGTGGATTCGATGGTGGCGGCAAAGAATTCGAtatt  <  2:57061/250‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       CGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATT  >  NC_000913/1347555‑1348024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑