| Read alignment evidence... | |||||||||||
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| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 377,402 | 0 | G | A | 30.0% | 35.8 / 16.3 | 30 | intergenic (+91/+133) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB |
| Reads supporting (aligned to +/- strand): ref base G (11/10); new base A (5/4); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCAT > NC_000913/377152‑377614 | aagctaaGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAgg < 1:137138/246‑1 (MQ=255) gAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCAcgcc < 1:193657/251‑1 (MQ=255) ccATTATCCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCg < 1:62538/251‑1 (MQ=255) ccATGATTCCCGCCAGCGTTGCCGATAAAATTTCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTGCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCTCAGATGCTTGATGCGACGCTATGCGCGTCTTATCAGGCCTTCGGTTTATTGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCg < 1:209404/251‑1 (MQ=255) cccGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGTATAAGACGTTTACGCCGCATCCGGCatttg < 1:283733/250‑5 (MQ=255) ccGCCAGCGTTGCCGCTAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTg > 2:180515/1‑251 (MQ=255) ccAGCGTTGCCGATAAACTTGCTAAGCGCGATGCCAGCAGTATAGTGTTGCCCAGCGCGATCAGCGCCGTAGAGCCGGAAGAAGACGACCCGTATGCCGACTACAAAGTGCCTGATGATTTGATGTGGTAAACATAGCTAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTAAGGTTTACGGGCGCAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCCATCGTgcgc > 2:128373/1‑251 (MQ=37) cAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgct > 1:76843/1‑250 (MQ=255) gCCGATAAAATGGCACAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGAGACGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGAGAAGCCGTGCGCAGATGCCTGCTGCGACGCTATGCGTGTCTGATTAGGCCGGGGGTTTATGGGCGAAGTGTAGCTCGGAGAAGACGTGTACGCCGCATCCGGCATTTGTgcgctgattcc < 2:63245/250‑8 (MQ=255) gATAAAATTGCTCAGCGCGATGCCAGCAGAATCGGGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTGGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATg < 2:24512/251‑1 (MQ=255) cTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCtg > 1:112727/1‑249 (MQ=39) tCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGAAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCtgac > 1:211399/1‑248 (MQ=38) cgATGCCAGCAGGATAGTGGTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGTCGCtgacgcgtct < 2:286957/251‑10 (MQ=38) cTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccgt > 1:268439/1‑232 (MQ=21) tCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGCTAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTACGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatatgtacgcgaaccgt > 1:166808/1‑231 (MQ=21) agagCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAg > 2:51390/1‑215 (MQ=255) agagCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAg < 1:51390/215‑1 (MQ=255) gagCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGAAGGCCgggaaagg > 2:221532/1‑245 (MQ=255) gggATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGAAGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGATTTGAGCGTCTTATCAGGACTACGGCTTACGGGCGAAGTGTAGGACGGCTAAGGCggt > 2:163757/2‑249 (MQ=38) aaGACGACCCCTATGCCGACTTCAAGGTGGCTGATGATTTGATGTGGTAAACAGAGATAAGTCGTGCGCAGAGTCCGGATGCGACGCTATGCGCGTCTTATCAGGCCTAGTTTTTATGGGCGAAGTTTATGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGGGC‑CATGCCTGATGCGACGCTTTGCGCGGCTTATCAGGCCTACGGCTTACGGGCTAAGTGTATGCCGGATAAGGCGTTCAcgcc < 2:270225/251‑1 (MQ=37) gCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGTTAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGTTAATGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACg > 1:173670/1‑251 (MQ=255) gatTTGATGGGGAAAACATAGATAAGGCGGGCGCAGAGGGCTGATGCGACGCTATGCGCGTTTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGGGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTc < 1:110157/251‑1 (MQ=38) atTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCtt < 2:68530/163‑1 (MQ=255) atTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCtt > 1:68530/1‑163 (MQ=255) ggagggtaggcgcGTCTTATCAGGCCGACGGTTTATGGGCGAAGGGTAGACCGGATAAGGCGTTAACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTAGCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCAGTCGTGCTAGTATCAACGCATATGCAGTTTAGTGGCGGGGTAGGCAATATGCTCGCCAATAAAACTGgaga < 2:159399/242‑1 (MQ=255) gACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTCGGCAATATGCTCGCCAATAAAAATGGCGAc > 1:158868/1‑251 (MQ=255) tATGCGCGTCTTATAAGGCATACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACACCGCATCCGGCAGTCGCGCGC‑CATGCCTGATACGACGCTTTGAGCGTCTTATCAGGCCTACGGCTTACGGGCGACGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCACAGCAACTTCAGTTTATTGGCCTGGGAGGCACTATACTCGGCCATAACACTGGACACACaat > 2:273707/1‑251 (MQ=25) tCAGGCCTACGGTTTCTGGGCGCAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTAGTATCAAAGCATATTCAGTTTATTGGCGTGGGAGGCAATATGCTCGCCCATAAAACTGGTGACAAAATAATATCTGTGATc > 2:45575/1‑251 (MQ=37) ccAGGCCTCCGGTTTAGGGGCGAAGTGTAGACCGGATAAGGCGTCCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTGGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATc < 1:204638/250‑1 (MQ=255) aGGCCTAGGGTTTATGGGCGAAGTGTAGACCGTATAAGGCGTGCCCGCCGCATCCGGCAGGCGTGTGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCAt < 2:243819/251‑1 (MQ=255) aCGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTg > 1:269886/1‑220 (MQ=255) | TAGCGAAGGTGAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCAT > NC_000913/377152‑377614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |