Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	606,417	IS4 (–) +12 bp	coding (956‑967/1119 nt)	ybdK ←	putative glutamate‑‑cysteine ligase 2

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	606417 =	0 (0.000)	25 (0.870)	23/484	0.3	100%	coding (967/1119 nt)	ybdK	putative glutamate‑‑cysteine ligase 2
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region
*	?	NC_000913	= 606428	0 (0.000)	17 (0.600)	17/482	0.5	100%	coding (956/1119 nt)	ybdK	putative glutamate‑‑cysteine ligase 2
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region

TAACCCTTAGCCACGCAGTAAATATGGCGGGATTAATTCAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/606650‑606417 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGATGG  <  NC_000913/4503515‑4503269                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   TAACCCTTAGCCACGCAGTAAATATGGCGGGATTAATTCAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCGGGAAAAAGTCGCCCCTTCCGCACTAATGCCGATCAGTTA                                                                                                                                                                                                                                         <  1:155869/250‑1                  GTAAATATGGCGGGATTAATTCAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATC                                                                                                                                                                                                                       <  1:63773/251‑1                            CGGGATTAATTCAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGT                                                                                                                                                                                                             >  2:17928/1‑251                                       CAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGTATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCG                                                                                                                                                                                                  <  2:28232/251‑1                                       CAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCG                                                                                                                                                                                                  >  1:28232/1‑251                                                  CCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCAC                                                                                                                                                                                       >  1:167684/1‑251                                                              GACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGAT                                                                                                                                                                           <  1:23452/251‑1                                                                         cgtttccACATAAGGAAAACGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCCCCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCCGTGGCTGGGTAAGAATCCGGAAAAGCCCACTTATTTCCGGCTTTTTTTATGa                                                                                                                                                                 >  2:200923/8‑249                                                                                ACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGAGGCTGTGTAAGAATCCGGAAACTCTCAATTGTTTCCGGATTTTTTTCTGCACATTGG                                                                                                                                                          >  1:231140/1‑250                                                                                         AAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTT                                                                                                                                                <  1:22204/251‑1                                                                                                         TATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAAAGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCCCACTAATGCCGATCAGTTAAGGTTCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCCCTTGATCTGGTATCCCGTT                                                                                                                                >  2:143396/1‑251                                                                                                            AAATTCAACCGTCTCTAGGCCTGTCGGTATGGGCTTGAAGGTGACATCACCGATCCGCACACTGGAGATCTTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTATCGCAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATTCATTTGCTGTGTAAGAATCCGTAAACGCTCACGTGTTTTCGGATTGTTTTATGCACATGGGACAGGCTCTTGATTTGGTATCCCGTTAC                                                                                                                              <  1:275816/250‑1                                                                                                                 CAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGGTTCCGGATTGTTGTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTC                                                                                                                         <  1:143396/250‑1                                                                                                                  AACCGGTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTC                                                                                                                       <  1:275074/251‑1                                                                                                                            AGGCCTGTCAATCTGGGCTTGAAGGCGTAATCACCGCTCCGCACACTGGAGATCGTAGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATAAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGCATCCGGAAACGCTTACTTGTTTCCGGCTTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGCTTATCTGCGTAACCC                                                                                                             >  2:299825/1‑251                                                                                                                                     GCTATGGGCTTAACGGCGTCATCACCGAACCGCACACTGGAGATCGTCACCCACTAAAGAAAGCTACCTTGCGATTGATGGAAAAAATCGCCCCTTCCGCAATAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTC                                                                                                    >  2:180903/1‑251                                                                                                                                       TATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACCGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTAACTTCTC                                                                                                  >  2:8883/1‑251                                                                                                                                       TATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTC                                                                                                  >  1:68970/1‑251                                                                                                                                                 AAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTACCGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGCTCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGCAACGCTCACTTGTTTCCGGCTTTTTTTATGCCCATTGGCCATGCTCTTTATCTGGTATCCCGTTACGATTCTCTGCTTAACCCCCTGCCTTCTCTGGGGGATT                                                                                         >  2:22318/1‑250                                                                                                                                                                           GGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCG                                                                <  2:68970/249‑1                                                                                                                                                                                 CGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTG                                                        >  1:11735/1‑251                                                                                                                                                                                   TCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCC                                                      >  1:81870/1‑251                                                                                                                                                                                        CGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCTTTGCCTTGCCGAATC                                                 <  1:62921/251‑1                                                                                                                                                                                                                           ATCGCCCCTTCCTCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCC              <  1:73413/251‑1                                                                                                                                                                                                                                       GCACTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGCAAGAATCCGGAAACGATCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATAAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGATGG  >  1:315716/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   TAACCCTTAGCCACGCAGTAAATATGGCGGGATTAATTCAGGCTACCGCCCACTGGTTACTGACGGAACGCCCGTTTAAACATCAGGAAAAAGATTACCTGCTGTATAAATTCAACCGTTTCCAGGCCTGTCGCTATGGGCTTGAAGGCGTCATCACCGATCCGCACACTGGAGATCGTCGACCGCTAACGGAAGATACCTTGCGATTGCTGGAAAAAATCGCCCCTTCCGCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/606650‑606417 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGATGG  <  NC_000913/4503515‑4503269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.