Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,350,184 (C)7→8 coding (276/654 nt) elbB ← low activity glyoxalase ElbB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,1841.C86.7% 76.5 / 5.1 30coding (276/654 nt)elbBlow activity glyoxalase ElbB
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base C (13/13);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAGGACACGGCACATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCC‑GGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCAT  >  NC_000913/3349935‑3350419
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            
gacacgacacGGCACAGGCTCAGAGCCCAGCTCTTCCAGCACTGCTCCGGTATCGATAGCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATGTTCGGCAGCAGCGCCGGAGCAATACACATAAAACCAAGCGTGTTCCCGGCATGATGCATCGCTTGTGACAGCTCCTTTAATTCGCGGTCAACGGTGCATTCGCTGCCAAGACTGGCAAAATTGCGTAAATTCTTCGCCGGTCCA‑A‑Actcc‑cccc                                                                                                                                                                                                                                              <  2:100451/251‑2 (MQ=255)
        acGGCACATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAACTTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTCCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑Caa                                                                                                                                                                                                                                      >  2:166377/1‑250 (MQ=255)
              caTGCTCCGCGCCCATCTCTTCCAGCAATTCTGCGGTCTCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGTCAGCATCGCCGGGGCAATACACATAAACCCAAGCGGTTTTCCGGCATGCTGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCACCGGTGCATTCGCTCCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCC‑CAATCAAcac                                                                                                                                                                                                                               >  2:305306/1‑249 (MQ=255)
                             tctcTTCCAGCACTTCTGCGGCATCGATATCAGTACCGATGGTCACACGCAGCGGGAAATCGAACCTTTTCGGCACCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGCGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCCAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCC‑CGCAACAATCAACGCATCCAGTTCAGCgcc                                                                                                                                                                                                                >  2:178387/1‑249 (MQ=255)
                                   ccAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCAGCCAGTTcatcggcatcgg                                                                                                                                                                                                           <  2:199108/250‑1 (MQ=255)
                                           tCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGc                                                                                                                                                                                                    <  1:228239/249‑1 (MQ=255)
                                                 gtaacGATATAAGTGCCGATGGGCAAACGCCGTGGGAAATCAAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTGATCCGGCCTGATGCGTCGCTTGTGCACGCGTCTTAAATTCCCGGGCAACGGTGCATACGCTACCAAGGTTGGCAAAATTGCTGAAAGTCGTCGCCTCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCAACCAGTTCGGCGGCATCGGCCTGGGCCAGAGGAc                                                                                                                                                                                            <  2:106944/247‑1 (MQ=255)
                                                 gTATCGATCTCAGTACAGATGGTAAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAGTAACCATAAAACCAAGCGGTTTTCCGGACTGATGCATCGCTTGTGCCCGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAACTTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCAACAAGTTCAGCGGAACCGGCCTGGGACAGAGGAc                                                                                                                                                                                            >  2:262806/1‑251 (MQ=255)
                                                                        caaccGCAGCGGGAAATCGAAAATTTGCTGGAGCATCGCCGGGGCAATACCCATAAAACCAAGCGGTTTGCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCGTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCATCGCATCCAGTTCAGCGGCATCGTCCTGGGCCAGAGGACGGATTTCACCGCGCTGTATTCg                                                                                                                                                                      <  2:268337/246‑1 (MQ=255)
                                                                                            aaaTTTTCGGCAGGATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTACTGCATCGCTTGAACCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCACGACTGGCAAAATTGCTTAAATTCTTCGCAGCGCCACA‑CCCCC‑CCC‑GGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGAAGGATTTCACCGCGCGTTATTCGTGCCGCTTCCATCAGCACAtt                                                                                                                                                 >  2:292910/1‑251 (MQ=255)
                                                                                                         caTCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTc                                                                                                                                    >  1:68033/1‑251 (MQ=255)
                                                                                                                     aaTACCCATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCa                                                                                                                        <  2:68033/251‑1 (MQ=255)
                                                                                                                        acacATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGt                                                                                                                      <  1:123382/250‑1 (MQ=255)
                                                                                                                               aaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACAtt                                                                                                                                                 <  1:124828/216‑1 (MQ=255)
                                                                                                                               aaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTAAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGCGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCCCAtt                                                                                                                                                 >  2:124828/1‑216 (MQ=255)
                                                                                                                                    aaGCGGTTTGCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCAGTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCTCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATa                                                                                                         <  1:178486/251‑1 (MQ=255)
                                                                                                                                           ttgccGGCCTGATGCATCGCTTTTGCCAGCCCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAAGTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCa                                                                                                   <  2:163829/247‑1 (MQ=255)
                                                                                                                                                  ccagatgactcgctaggtccAGCGTCTTTAATTCACGGCCAACGTTGCATTTGCGACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑ATCCCCC‑CCC‑GTCA‑CAATCAACGCATCCAGTTCTGCGGCATCGGCCTGGGCCATAGGACGGATTTCCCCGCGCGTTAATCTTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACACCAACctgctg                                                                                           <  2:88941/233‑1 (MQ=255)
                                                                                                                                                  ccTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCACGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACctgctg                                                                                           >  2:286180/1‑251 (MQ=255)
                                                                                                                                                                 gtgCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCaa                                                                             >  2:88871/1‑250 (MQ=255)
                                                                                                                                                                  tgCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCCAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGATTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACTTCAACCTGCTGCTTATCCGGTGCaaac                                                                           >  2:117412/1‑250 (MQ=255)
                                                                                                                                                                        cgcCTTTAATTCACGGTCAACGGTGCATTCGCAGCCAAGAATGGCAAACTTCCTTAATTTCTTCGCCCTGAAA‑A‑ACCCCTCAC‑CGCA‑CAATCATCGCATACCGTTCAGCGGGCTCGGCCTAGGCCCGCGAACGCATTTCAACGCGCGTTATTAGTGCCGCTTCCATCAGCACATTGAGCGATTCCGGCCCCACTTCGCCAGTTACATGGTTGATAACATCCACCTACTGCTTATCCGGTGCCAAGCCGAcc                                                                     >  1:307363/1‑251 (MQ=255)
                                                                                                                                                                             ttAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTg                                                                >  2:217208/1‑251 (MQ=255)
                                                                                                                                                                                 ttCACGGTCAACGGTGCCTTAACTACCACGAATGGCACAATAGATTAAATTCTTCGCCGAGCCA‑A‑CCCCC‑CCCCGCCA‑CAATCAACGCATCCCGTTACGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCCCGCGTTATTCGTGCCGCTTCAATCAGCCCATTGCGCGTTTCCGTCATCGCTTCGCCAGTTACATGCTTGATAACATCAACCTTCTGCTTATCCGGTGCAAAGCAGACCGCCTccgc                                                             >  2:314428/1‑250 (MQ=255)
                                                                                                                                                                                         cAACGGTTCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGc                                                     <  1:148981/250‑1 (MQ=255)
                                                                                                                                                                                                ccATTCGCTTCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACTCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGTATTGCACCGCGCGTTATTCTTGCCTCTTCAATCAGCACATTTCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCATCCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTTAAATa                                             <  2:22735/250‑1 (MQ=255)
                                                                                                                                                                                                         aCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTaacaa                                     <  1:88871/250‑1 (MQ=255)
                                                                                                                                                                                                          ccAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGCGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTaacaacc                                   >  2:146920/1‑250 (MQ=255)
                                                                                                                                                                                                                cTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAac                              >  1:203751/1‑250 (MQ=255)
                                                                                                                                                                                                                        aTTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGCCGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAACACCGCTTc                      >  2:182691/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gcgcCA‑A‑ACCCC‑CCCCGGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCAt  <  1:286180/251‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            
GACAGGACACGGCACATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCA‑A‑ACCCC‑CCC‑GGCA‑CAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCAT  >  NC_000913/3349935‑3350419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑