| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,257,679 | +C | coding (332/1332 nt) | cyuP ← | cysteine detoxification protein CyuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,257,679 | 1 | . | C | 81.8% | 58.7 / 7.5 | 22 | coding (332/1332 nt) | cyuP | cysteine detoxification protein CyuP |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (7/11); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑C‑AGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACAATCCCCGCG > NC_000913/3257433‑3257861 | gcgACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑c‑cc‑ccagt < 1:219046/251‑5 (MQ=255) accaccAGCAGCGTGGTCAGCACCATGCCGGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCGTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCAGCTAACAGGATG‑C‑CC‑CCAGtttt < 1:98478/251‑1 (MQ=255) ccgccaGCAGCGTGGTCAGCACCATGCCGGTCGAAATTGTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCATACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGGATGCAGGTAGGAAGCTCGATCGTTGGTGAGGGCGGTGTAATAGACGATCGTCCAAATCTCCAGCTTTACGAAATAGAGCTCGCCGAAGAGGATG‑CTCC‑C‑GGttttt < 2:139321/248‑1 (MQ=255) agcTTGGTCAGCACCATGCCGGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACGGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAAt < 2:268920/251‑1 (MQ=255) ccATGCCGGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCTCCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACATCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTGGTACCTAAATAACCGCTAATGAc < 2:135567/251‑1 (MQ=255) gCCGGTCGAACTTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTc > 2:92471/1‑251 (MQ=255) ttGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCCGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGCAGGTATGCAGGCAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATACAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CCAC‑AGTTTTTACCTAAATAACCGCTAATGACGCTCGGGAAATCTTTa > 1:139425/1‑250 (MQ=255) gTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGt < 1:84308/251‑1 (MQ=255) cGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAAGAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAAc < 1:53537/251‑1 (MQ=255) cGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAAAATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAAACGCTAATGACGCTCGGGGAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATaaa > 2:13418/1‑250 (MQ=255) aTGCAAATCAGTACCAGAACATAAAAGGGACTGTCTGAAAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGCATAGACGAACATCCCAATCACCAGCATTACGAAATAGAGAGCACATAAAAGGATG‑C‑CC‑CCAGTTTTTAACTAAATAACCGATAATGACGCTCGGGTAATCTTTACATTCTGGGGATTCTGCCAGCGTATTAATAAACAATCGCTGa > 2:190546/1‑250 (MQ=255) cAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATAc < 1:92471/251‑1 (MQ=255) aGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCg < 1:190546/251‑1 (MQ=255) cATAAAAAGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGAAGGTAGGGAGCACTATCGTTGGTGATGGCGGGGGAAGAGAAGAAAATACCAATCAACAGCATTTCGAAATAGGACGCAACGAACAGGATG‑C‑CC‑CCAGTTTTTAACTAAATAACCGCAAAAGACGATCAGATAATCTTTACATTCTGGAGATTCTGCCAACGTATTAATAACCAATCGCTGAAACAGATAAATTACCGGGt > 2:15084/1‑250 (MQ=255) aaaaGGGCCTGTCTGACAGCAACCACTCCGTCACGCCCAAGGTATGCTGCTACGACGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGCACTAGAGCGCACCTAACAGGATGAC‑CC‑C‑AGTTTTTACCTAAATAAACGCTCATGAAGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACCTTGCCGGGTAAcc > 2:158190/1‑251 (MQ=255) cAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CCAC‑AGTTTTTACCTAAATAACCGCTAATGACGCTCGGGGAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACAAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGAcc > 2:25735/1‑251 (MQ=255) aGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCggg < 1:221138/251‑1 (MQ=255) gTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACa < 1:158190/251‑1 (MQ=255) gATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTc > 1:223283/1‑130 (MQ=255) gATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTc < 2:223283/130‑1 (MQ=255) ggCGGTGGAATAGACGAACATCCCAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACAATCCCcgcg > 1:264731/1‑251 (MQ=255) tAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTg < 2:30560/172‑1 (MQ=255) tAACAGGATG‑C‑CC‑CCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTg > 1:30560/1‑172 (MQ=255) | GCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATG‑C‑CC‑C‑AGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACAATCCCCGCG > NC_000913/3257433‑3257861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |