| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,112,282 | (C)5→6 | coding (768/996 nt) | yghF ← | putative type II secretion system C‑type protein YghF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,112,282 | 1 | . | C | 88.9% | 83.6 / 1.9 | 27 | coding (768/996 nt) | yghF | putative type II secretion system C‑type protein YghF |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (6/18); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAATGTCGTGGCGCGCACCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCC‑GGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCACTGACGGCTTTTTTGTTGGTCACAGCAACAGTGGAACGTTCCTCTTCTGCCAGACTCAGACGCTCTATTTTTCCCTGATAACGC > NC_000913/3112061‑3112528 | tgcttcCAGGAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAAGTTCGTGGCGCGTGCCCGTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAAGCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCGTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCTTC‑CCCCC‑GGCTTCGCTGCATAACCGACA‑ATcccc < 2:111975/248‑1 (MQ=255) cAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAATGTCGTGGCGCGCACCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTAc < 1:28189/251‑1 (MQ=255) gacaggTCGGTAGCCCAGCGCGATGGAAATGTCGTGGCGCGCACCCTAGCGTAAAACCGGAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGTGGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGATAACGATCT‑G‑CCCCCCGGCTTCGCGGCATAACCGTCA‑ATCCCCTCCTTACGCACAGGCGTAAGc < 2:129963/246‑1 (MQ=255) aggcGGTAGCGCAGCGCGATGGAAATGACGTGGCGCGCACCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCTTAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTgg < 1:76821/247‑1 (MQ=255) ggTAGCGCAGCGCGATGGAAATGTCGTGGCGCGCACCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGatat > 1:274866/1‑250 (MQ=255) ggcgcagcgcGATGGAAATGGCGTGGCGCGCCCCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAAAGGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAACCCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGtt < 2:94208/250‑1 (MQ=255) agcgcagcgcGATGGAAATGTCGTGGCGCGCACCCTTGCGTAAAACCGTACGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGtt > 2:45788/1‑251 (MQ=255) gATGGAAATGTCGTGGCGCGCACCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTc < 1:45788/251‑1 (MQ=255) gTGGCGCGCACCCTTTCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACTGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTc < 2:274866/251‑1 (MQ=255) cTTGCGTAAAACCGTAAGATGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑GACCCCC‑GGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCCCAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTg > 2:90508/1‑250 (MQ=255) aaGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATAAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCgg < 2:142166/250‑1 (MQ=255) tGAATGGAACCCATTGAAGGTAACTGCCGCATCAGAGCAAGCATTGCGCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGAGCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCGTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGAtc < 2:223899/250‑1 (MQ=255) gAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTGTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGAtct < 1:153071/250‑1 (MQ=255) cATTGACGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCCGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGACTGACGCACGGCAGCCGGGATCTCAACTGGcac > 2:118569/1‑250 (MQ=255) aGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑GTCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGtt > 2:31911/1‑181 (MQ=255) aGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑GTCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGtt < 1:31911/181‑1 (MQ=255) aaCTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGAcagca < 1:62356/250‑1 (MQ=255) cGCATCAGAGCAAGCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAg < 2:204345/251‑1 (MQ=255) agagCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCgg < 1:74364/180‑1 (MQ=255) agagCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCgg > 2:74364/1‑180 (MQ=255) cGTGTATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATAGCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTc < 1:172913/249‑1 (MQ=255) ggATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCAc < 1:165564/251‑1 (MQ=255) gagcAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCACt < 2:221538/248‑1 (MQ=255) tCAGTGAAATCTTGCCGATTGAGAGCAATGGAGATATAGTTTTACTTGAAACAGCTGGAATAGCAAAGAAAAAGATCA‑GACACAC‑GGCTTCGATGCATAACAG‑CACAGCCCCACCTTCCGAACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTGTCGCCAGCGCCTGACAATAGGCAGCCAGGATCTCACATGGAACACTGTCAGCAGGCTCAGCAACAgcttgcttagcatcggcacagg > 2:109179/1‑237 (MQ=255) aacccTGTTGATTTAGCGCAATGGCGATATCGCCTTTCTTGAAACCGCGGGCATCGAACCGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAAGTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACATCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCACTGACGGCttt < 2:239507/248‑1 (MQ=255) atatCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCCCGGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCCCAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCACTGACGGCTTTTTTGTTGGTCACAGCAACAGTGGAAc > 1:18048/1‑251 (MQ=255) cgcctcccttaaaacctcggtcaccGAACAGACTACGATCT‑G‑CCCCCCTGCTTCGCTGAATACCCGGCA‑ATCCCCTCCGTACGCCCAGGCGTAAGCTGTATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACTCACGGCAGCCTGTATATCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTGGCTTGGCTTCGTCGCTGACGGCTTTTTTGTTGGTCACAGCAACAGTGGAACGTTc < 2:165245/227‑1 (MQ=255) tgcccccc‑GGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTTGATATAGTTAAACCTTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGTCCCACTGACAGCAGGCTACGCAACAGCTTTCTTTGCTTCGTCACTGACGGCTTTTTTGTTGGTCACAGCAACAGTGTCACGTTCCTCTTCTACCATACTCATACGCTCTATTTTTCCCtactac > 2:278971/4‑246 (MQ=255) ccc‑GGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCACTGACGGCTTTTTTGTTGGTCACAGCAACAGTGGAACGTTCCTCTTCTGCCAGACTCAGACGCTCTATTTTTCCCTGATAACgc > 2:166074/1‑249 (MQ=255) | TTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAATGTCGTGGCGCGCACCCTTGCGTAAAACCGTAAGTTGAATGGAATCCATTGAAGGTAACTGCCGCATCAGAGCAATCATTGCTCGTGGATCAGTGAAATCCTGCTGATTTAGCGCAATGGCGATATCGCCTTCCTTGAAACCGCTGGCATCGAACAGAGAACGATCT‑G‑CCCCC‑GGCTTCGCTGCATAACCGACA‑ATCCCCTCCTTACGCACAGGCGTAAGCTGGATATAGTTAAAAATTTTCTGCGGATCTTTCGCCAGTGCCTGACGCACGGCAGCCGGGATCTCAACTGGCACACTGACAGCAGGCTCAGCAACAGCTTGCTTTGCTTCGTCACTGACGGCTTTTTTGTTGGTCACAGCAACAGTGGAACGTTCCTCTTCTGCCAGACTCAGACGCTCTATTTTTCCCTGATAACGC > NC_000913/3112061‑3112528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |