Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,067,097 (T)6→7 coding (77/897 nt) ygfI ← putative DNA‑binding transcriptional regulator YgfI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,0971.T93.8% 48.8 / ‑2.5 16coding (77/897 nt)ygfIputative DNA‑binding transcriptional regulator YgfI
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (11/4);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CAATCACAATTTCTCGTGAACGACGCCCTGAGTTATGGATTTCATTATCAATCGCAGAAAGCCGTTGATAAACCGGGAACAGTTTTTGATACAGTTCCTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTT‑CTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGT  >  NC_000913/3066882‑3067326
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     
cAATCACACTTTCTCGTGAACGACGCCCTGAGTTATGGATTTCATTATCAATCGCAGAAAGCCGTTGATAAACCGGGAACAGTTTTTGATACAGTTCCTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCta                                                                                                                                                                                                      >  2:212656/1‑250 (MQ=255)
                tGAACGACGCCCTGAGTTATGGATTTCATTATCAATAGCAGAAAGCCCTTGATAAAACGGGAACAGCTTTTGATACAGTTCCAGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGATGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTATTAAAATGAAATTgcgc                                                                                                                                                                                     >  1:28662/1‑251 (MQ=255)
                      ccgCCCTGAGTTATGGATTTCATTATCAATCGCAGAAAGCCGTTGATAAACCGGGAACAGTTTTTGATACAGTTCCTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCGGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCttt                                                                                                                                                                               <  2:192274/250‑1 (MQ=255)
                      ccgCCCTGAGTTATGGATGTCATTATCAATCGCATAAAGCCGTTGATAAACCGGGAACAGTTTTTGATACAGTTCCTGTCCTGCGTTATTGAGGCTGATATTATGATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCttt                                                                                                                                                                               <  2:271484/250‑1 (MQ=255)
                                   tGGATTTCATTATCAATCGCAGAAAGCCGTTGATAAAACGGGAACAGATTTTGATACAGTTACTGTCCGGCTTTATTGAGGCGGATATTCTTATCTATGCGGGTAAATAGCGGATCACCAATATGTTCATCTAGCGCGGCAATGCTTTGACCAAATGGAGTAGCCGTCATATGGAT‑TTTTTTCTTCCGCACGGGCAATATTAATGGTTTGTGCTAATAAAATGAAATTTCGCATCTTTTTCGAAATaaaa                                                                                                                                                                   >  1:218101/1‑250 (MQ=255)
                                                 aaTCGAAGAAAGCCGTTGATAAACCGGGAACAGTTTTTGATACAGTTCCTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCACTTTGTTCTTCCAGCGCGGCGATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCCATATTATTGGTTTGCTCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCAtccccctc                                                                                                                                                    >  1:218578/1‑251 (MQ=255)
                                                                   gtaacccGGGAACAGCTTTTGATACAGTTCATGTCGGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTACATAGCGTATCGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTATGCCGCCCGGGCAATATTATTGGTTAGCGCTACTAAAATGAAATTGCGCATCTTTTTCGTAATAAAACTTTCCATCCCCCTCCCCCGCATCCGTGTCCGc                                                                                                                                  >  2:41693/6‑251 (MQ=255)
                                                                         cGGGAACAGTTTTTGATACAGTTACTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGATCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAAcc                                                                                                                            >  1:269199/1‑251 (MQ=255)
                                                                           ggAACAGTTTTTGATACAGTTCCTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGt                                                                                                                          >  1:259713/1‑251 (MQ=255)
                                                                                      tGATACAGTTCCTGTCCGGCTTTATTGATGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTGCTTCCAGCTCGGCAATGCTTGTACCAAATGGCGAAGCCGTCATATGGATGTGTAT‑CTGCCGCCCCTGCAATATTATTTGTTTGCTCTAATAAAAGGAAATTGCGCATCTTTTTCGAAATATAAAGGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGc                                                                                                                <  1:296115/250‑1 (MQ=255)
                                                                                          aCAGTTCCTGTCAGGCTTTATTGAGGCTCATACTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCATCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATc                                                                                                           >  2:55005/1‑251 (MQ=255)
                                                                                                                    cTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAATCAGCCTATCCTTGATGAAACCGCGAGCAAAGAt                                                                                   >  1:106474/1‑249 (MQ=255)
                                                                                                                    cTGATATTATTATCTTTGCGGGAAAATAGCGTATCGCCAATTTGTTCTTCCAGCGCGGCAATGATTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATACAATGAAATTGCGCATCTTTTTCGAAATAAAACTGTCCATCACCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAgctcg                                                                                 >  2:134406/1‑247 (MQ=255)
                                                                                                                                                    ataGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTttacagaaaattac                                                  <  1:165315/250‑1 (MQ=255)
                                                                                                                                                                          gcgGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGt                           >  1:235337/1‑251 (MQ=255)
                                                                                                                                                                                                   aaGCCGTCATATGGAT‑TTTTTTCTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGTCAATAGCGGGTAAAGGCATTAGCCCGACGAATACGt  <  1:276047/251‑1 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     
CAATCACAATTTCTCGTGAACGACGCCCTGAGTTATGGATTTCATTATCAATCGCAGAAAGCCGTTGATAAACCGGGAACAGTTTTTGATACAGTTCCTGTCCGGCTTTATTGAGGCTGATATTATTATCTTTGCGGGTAAATAGCGTATAGCCAATTTGTTCTTCCAGCGCGGCAATGCTTTTACCAAATGGCGAAGCCGTCATATGGAT‑TTTTT‑CTGCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGT  >  NC_000913/3066882‑3067326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑