| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,917,324 | (A)5→4 | coding (2268/2757 nt) | barA → | sensor histidine kinase BarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,324 | 0 | A | . | 90.0% | 100.2 / 5.5 | 30 | coding (2268/2757 nt) | barA | sensor histidine kinase BarA |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (11/16); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
GCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACT > NC_000913/2917088‑2917557 | gCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GCGAAGCTGCTTGgc > 2:82027/1‑251 (MQ=255) aaCCTGAAACTTATCGGCCCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTTAACGCGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATCCAAATGCCGGACATGGATGCCATTCGGGCCGGCGAACTCCGCCCCCATCTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATTCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGgcg < 1:126356/249‑1 (MQ=255) gAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGAt < 1:235235/251‑1 (MQ=255) tATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCg > 2:97480/1‑250 (MQ=255) gcGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGAt < 2:72269/249‑1 (MQ=255) gATATGGTGCAACAGGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGAtt < 2:81564/251‑1 (MQ=255) ggAACTTTGCGATAGCTGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTGGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑TAGAAGCTGCTTGGCGCAGGGATGAGGGTGTATCTGGCGAAACCGATTAAAGTGGAGCGATTGc < 2:291250/250‑1 (MQ=255) aaCTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCAt > 1:84791/1‑250 (MQ=255) aTAGCGGGCATCAGACGGATGAACGGGCGAAACAGATGCCGTGCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCCAATCCCGCATCAGCAACAAACGCCGGTTATCGCGGGAACGACGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGAGCAGGGATGAGCGATTATCTGGCGAACCCGATTgaaagaaggcgcttgacaaatttgttgt > 2:104631/1‑226 (MQ=255) gAACGGGCGAAACAGAGGCCGCGCGATTTGAACTTAAGGGATATTCAAATGCCGGACCTGGATGTCAGCCGGGCCTGCGAACTCATCCACCAACTCCCGGACCAGCAACAACCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGGAGGTATGAGCTATTATCTGGCGAAACCGATTGAAGAAGAGAGAGGGCATAATTTGTTGTGGCGCTATAAGCCGGGCAg < 2:198592/250‑1 (MQ=255) cGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAttt > 1:59646/1‑251 (MQ=255) gATCTTAATGGTTAGTCAAATGCCGGACATTGATGGCATTCGGGCTTGCGAACTCATACACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCAGGCAATGGCCCGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATAAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACACGCTTAGCAGCGGAATTTCCTCACGCGTCGTGACGcact > 2:210982/1‑247 (MQ=255) tCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTACCGGCGCATGCAATGGCCGGGCCAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACAGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGGAGCGGTATTTCCTCTCGCTTCGTGACGCCCGa > 2:119656/1‑249 (MQ=255) tCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATtgt > 1:138905/1‑251 (MQ=255) atggatggCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAAt < 2:138905/251‑1 (MQ=255) gatggCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCg < 1:265989/251‑1 (MQ=255) aTTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAGCGCCGGTTATAGCGGAAACGGCGCATGCAATGGCCGGG‑AAAAAGAGAAGCTGCTTGGCGCAGGGAGGAGCGATTATCTGGCGAAACCGTTTGAAGAAGAGCGATTGCATAAATTGATGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGGGCCGCCCGAAGTCAAAGAAATTGTGGGGAACCCGAATGAGAGCCTc > 2:138772/1‑251 (MQ=255) gCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTAGCGCGGTAACGGCGCATGCAATGGCCGGTCAAAAAGAGAAGCTTCTTGGCGCAGGGATGGGCGATTATCTGGCGAAACCGATTGAAGAAGTTCGATTGCATAATGTGTTGTTGCTCTACAAGCCTGGCAGCGGTATTTCCTCTCGCTGCGGGACGCCCGAAGTCAACAGAGGTGGGGTGACCCCGAATGCGATCGTCGgctggca < 2:143355/250‑1 (MQ=255) ccccGCATCAGCAACAAACGCCGGTTAGCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGgcag < 2:84791/250‑1 (MQ=255) cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGaaa < 1:82027/250‑1 (MQ=255) cAGCAACAAACGCCGGTTATCGCGGAAACGGCGCATGCAATGGCCGG‑CCAAAAGAGAAGCTGCTTGGCGCAGGGATGAGAGATTATCTGGCGACACCGATTGAAGAAGAGCGCTTGCATAATTTGTTGTTGCGCTACAAGACTGGCAGCGGTATTTCCTCACGCGTCGTGACGACCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAAATGACACTACGCAAGgcagcaagcaa > 2:106488/1‑245 (MQ=255) aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGaaaaa < 1:299526/251‑1 (MQ=255) caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGcc < 1:247013/139‑1 (MQ=255) caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGcc > 2:247013/1‑139 (MQ=255) accaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCGGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCg < 2:84808/249‑1 (MQ=255) acaaACGCCGGTTCCCGCGGTAACGACGCATGCAATGGCCGGACAAAA‑GAGAACATGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGCCCCTCGCCTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAAc > 2:291232/1‑248 (MQ=255) ggTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAgcgcg < 1:119771/251‑1 (MQ=255) aTCGCGGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGat < 1:97480/251‑1 (MQ=255) gcgGTAACGGCGCATGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATAtg < 2:59646/251‑1 (MQ=255) tGCAATGGCCGGGCAAAA‑GAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACt > 2:109887/1‑251 (MQ=255) | GCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACT > NC_000913/2917088‑2917557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |