Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,795,610	(A)5→4	coding (1358/1401 nt)	gabP →	4‑aminobutanoate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,795,610	0	A	.	100.0%	66.4 / NA	16	coding (1358/1401 nt)	gabP	4‑aminobutanoate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/6);  total (10/6)

TTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGGTGAAT  >  NC_000913/2795374‑2795853                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ttattttGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGc                                                                                                                                                                                                                                       >  1:84846/1‑250 (MQ=255)                      acaGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTAGCTAGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGACAGTGATGCTATTCCGTCCGACGCAACAGTTAGAAGTGATCTCCACCGGCTGATTAGCGCTAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTTGAAAA‑GATGGTATTATGGCAAAAAACACACTTTCATAATAc                                                                                                                                                                                                                 >  1:144645/1‑251 (MQ=255)                          ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACgcgc                                                                                                                                                                                                             >  1:54962/1‑251 (MQ=255)                                       aaaTTCTGCGAGCAGAGGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCtgatcatgata                                                                                                                                                                                                  >  1:11149/1‑248 (MQ=255)                                                         gaagCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCAt                                                                                                                                                                              <  1:154957/251‑1 (MQ=255)                                                           agCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGTGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGAGCAGGAGTCACACCATGACCATTa                                                                                                                                                                            <  1:274427/251‑1 (MQ=255)                                                                                           gTGGCTTACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAAc                                                                                                                                            >  1:84401/1‑251 (MQ=255)                                                                                                    cTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGAAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAGGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGGTCATAATACGCGATGATCATGATCAGGAGTAACCCCATGACCATTACGTCTCTGGAGGGCTATCGCTGGCTGTAGAACGATattatt                                                                                                                                   >  1:96566/1‑251 (MQ=255)                                                                                                             cATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTaa                                                                                                                           <  1:145964/250‑1 (MQ=255)                                                                                                                           ccTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATTGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATg                                                                                                             <  2:39780/250‑1 (MQ=255)                                                                                                                           ccTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATg                                                                                                             >  1:39780/1‑250 (MQ=255)                                                                                                                                     ggTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTTCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGAGTATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGa                                                                                                  <  1:38341/251‑1 (MQ=255)                                                                                                                                                     cgtccgGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACAt                                                                                  <  2:54962/251‑1 (MQ=255)                                                                                                                                                                                             taGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGctct                                          >  1:130456/1‑251 (MQ=255)                                                                                                                                                                                              aGCGATAGGGATTCTCTGTACCGTGCCAATCATGGCTCGCCGGAAAA‑GCAGGCATTGTGGCAAAAAACACCCGATCATAATACGCGCTGACCATGATAGGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCACTGGCTGAAGAACGATATTATTCGCGGTAATTTTCACCCGGATGAACAATTTCGAATGAGTTTGCTGAAATCGCGTTATGCCCATGGCTCTGGGCCGTTACTGGAAGCTCtt                                         >  2:148207/1‑251 (MQ=255)                                                                                                                                                                                                  aTAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAA‑GCTGGTATTGTGGCAAAAAACCCCCGTTCCTAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCgctgg                                                                                                                                                     >  2:51418/1‑139 (MQ=255)                                                                                                                                                                                                                                      ctggaaaaGCTGGTCTTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGACGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGGTGAAt  >  1:95105/5‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGGTGAAT  >  NC_000913/2795374‑2795853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑