| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,111,259 | Δ1 bp | coding (718/900 nt) | rfbD ← | dTDP‑4‑dehydrorhamnose reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,111,259 | 0 | G | . | 95.5% | 86.4 / ‑2.4 | 22 | coding (718/900 nt) | rfbD | dTDP‑4‑dehydrorhamnose reductase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (13/8); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTC > NC_000913/2111049‑2111507 | gaccaTCAAGAACACGATGCAAAACCTATTCACTTACTGTAGTCGTAAATACTTCATTGAGCATTCGTTTCAAGCAAACCTGCCAGTCAGGCAAGCCAAGCGCAAAGTTCTGCTGCAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGAAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCGAGGG‑TATGCCTGCTTTGCGCGCCTCTTCAAAATCCAGAGCAGCAt > 2:194033/2‑251 (MQ=255) aTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAACTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGAGCCTCTTCAAAAACCAGCGCAGCATAATc > 1:36082/1‑251 (MQ=255) cGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGTCAAGACAAGCGCAAAGTGCGGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCTCGCCTCTTCAAAAACCAGCGCAGCATAATCGTAc < 1:194033/251‑1 (MQ=255) gATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTtg < 1:27661/251‑1 (MQ=255) aTTAAATGGCTGGAGTCGTAAATAATTCATTGATCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGGTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTg < 2:21891/250‑1 (MQ=255) aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCt > 1:199706/1‑251 (MQ=255) aTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCCAGAAAAGACCAACGTTCTGCTGAAATTTTCCTGTATTAAGACGAGAGTTATGAGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGATGAGATTGTTGCGTGCAAGGG‑AACGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTAACAGGTTGTGGTACCACTGGCTACAAAATGGCACAAGCCTGCgcaatc > 1:291258/1‑246 (MQ=255) ttCACGACAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGATGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAGGACGAGCTGGTGTAGGGTAGGCTGTTGTTGGAA‑CTGCGTTGAGCTTGATGAGTGCAAGGG‑AATGCCTGCTTTGAGCGCCTCTTCAAAAACAAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGAACAAGCCTGCGACATCCGGTTTATTCAGt > 1:144877/1‑251 (MQ=255) aCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTg > 2:54667/1‑146 (MQ=255) aCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTg < 1:54667/146‑1 (MQ=255) ccAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTGGGTA‑CGGCGTTGAGCTTGTTGAGTGCAAGGG‑AAGGCCTGCTTTGCGCGCCTCTGCAAAAACCAGCGCAGCATAATCGGACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACAGCCGGTTTATTCAGTGCGACACGGATGGc < 2:94785/251‑1 (MQ=255) aGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGAACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGAATGTGc > 1:128637/1‑251 (MQ=255) aGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑GGTAACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTTCCACTGGCTACCAAATGGTACAAGCCTGCGACAGCCGGTTTATTCAGTGCGACACGAATGGCATGTGc < 1:143981/251‑1 (MQ=255) cgcAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGc > 2:127799/1‑200 (MQ=255) cgcAAAGTTCTGCTGAAATTTTTCTGTAGTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGGTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACGGGCTACCAAATGGTACAAGCCTGc < 1:127799/200‑1 (MQ=255) aaaGTTTTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagcc > 1:85089/1‑249 (MQ=255) gTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGGCGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACAAGGTTGTGGTACCACTGGCTACCAAATGGTACACGCCTGCGAAATCCGGTTTATTCAGCGCGACACGCATGGCATGTGCTGTACAATcagccagc > 1:272500/1‑249 (MQ=255) aTTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCAcc > 1:237195/1‑251 (MQ=255) gCGAGAGTTATGTGGACGACGAGCTGGTGTAGGCTAGGCTGCTGTTGGTA‑CTGCGTTGAGCTTGTTGAGCGCAAGGG‑AATGACTGCTCTGCGCCCCTCTTCAAAACCCAGCGCAGCATAATCGTACCAGGTTGTGGTCCCACTGGCTACCAAATGGCACAAGCCTGCGACATCCGGTTTATTCAGTGCAACACGACTGGCATGTACTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTg > 1:313606/1‑251 (MQ=255) gAGCTTGTTGAGTG‑AAAGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGCCATCCGGTTTATTCAGGGCAACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGATAATAACCGCTACTTCTTCACGCTCTTTTGCCAGACGTAAAATAGTTTTggg > 2:249699/1‑250 (MQ=255) gttgAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGtt < 2:85089/251‑1 (MQ=255) tGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGtt > 1:59467/1‑234 (MQ=255) agcaagggAATGCCTGCTGTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTCCCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGtt < 2:59467/229‑1 (MQ=255) caagggAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttcctt > 2:265179/4‑251 (MQ=255) gggAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttccttttc > 1:127435/1‑251 (MQ=255) | CACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA‑CTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTC > NC_000913/2111049‑2111507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |