Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,563,394	(G)5→4	coding (2340/2400 nt)	dosP ←	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,563,394	0	G	.	100.0%	26.7 / NA	8	coding (2340/2400 nt)	dosP	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (2/6);  total (2/6)

CAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTC‑AAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGG‑TTAAATTGAGGC‑TTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACT  >  NC_000913/1563152‑1563570                                                                                                                                                                                                                                                   |                                                                                                                                                                                    cAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCTGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCGGAAATCGCAGTATTTAAGTTACAGGGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCTGTAACACTCGGCTTAGTCAGTCTGGAATTTCTTCGGCGGGTAGGGG‑CGGGAAAAg                                                                                                                                                                            <  1:200132/251‑1 (MQ=255)                                                  gCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAACCCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGG‑CGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTC‑AAAt                                                                                                                           <  1:182142/250‑1 (MQ=255)                                                                                                            gTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGG‑CGGGGAAAGAAAAATCCCCGGATAAAACGACAGGGGGTCTTTGGTAGCCTCTCCAAATT‑CTCTTTTGTTTTTGCGCCTTCCCCCCCGACGGGTTAAAttagggtttttcccctgct                                                                >  1:59200/1‑234 (MQ=255)                                                                                                                     aTCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGG‑CGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTC‑AAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGG‑TTAAATTGAGGC‑TTTGCCCAATGCTGGTAATGGc                                                     <  1:257856/251‑1 (MQ=255)                                                                                                                                     gaaagggtCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGG‑CGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTC‑AAAGTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGG‑TTAAATTGAGGC‑TTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAgg                                     <  2:111022/245‑1 (MQ=255)                                                                                                                                                           aCCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGG‑CGGGGAAAGAAAAATTACCGGATAACACGGCCGAGGGTCTTTTGTAGCATCTC‑AAATTTATCTTTGGGTTTGACACCTTCCCCCACCACGG‑GTAAAATTAGGCGTTTGCCCA‑TGCTGGGAATGGGTTTAAGTAAGGGAAgggagcgctttttggtgtgacaa                >  1:173613/1‑229 (MQ=255)                                                                                                                                                                        ggCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGGGGGTAGGGG‑CGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTC‑AAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGG‑TTAAATTGAGGC‑TTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTGTTTCGGTCAGACAACGATCGACAAAACt  <  2:59200/251‑1 (MQ=255)                                                                                                                                                                                                                   aTCCAGCCTGGAATTTCTTCGGCGGGTAGGGG‑CGGTAAAAGAAATATCCCTGAATAGCGCGACAGTGGATCTTGCGTAGCATCTC‑AAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGG‑TTAAATTGAGGC‑TTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACaa                <  2:290112/194‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                    CAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTC‑AAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGG‑TTAAATTGAGGC‑TTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACT  >  NC_000913/1563152‑1563570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑