Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 178,000 (T)6→5 coding (463/801 nt) btuF ← vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913178,0000T.100.0% 118.3 / NA 27coding (463/801 nt)btuFvitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/11);  total (16/11)

TAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGATCGGGTTTCAGCGCGACAATGC  >  NC_000913/177754‑178245
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
tAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGtt                                                                                                                                                                                                                                                  >  1:168132/1‑251 (MQ=255)
                            gTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCTTGCTACCCCCTGTTCGCGGTTTCCTTGCGGCCGGGGAACCCGTCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGTTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTgcgc                                                                                                                                                                                                                      <  2:168132/251‑1 (MQ=255)
                                                      ccggtccgCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCgg                                                                                                                                                                                            >  1:216743/1‑251 (MQ=255)
                                                               cTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCgg                                                                                                                                                                                   >  2:223415/1‑251 (MQ=255)
                                                                    aTGACAAGCGCCGGTGGCGGGCGTTCTAACACCTGTTCGCGGCTAACTTGCGGCCGGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTg                                                                                                                                                                              <  1:76312/251‑1 (MQ=255)
                                                                          aTCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGt                                                                                                                                                                        >  2:121812/1‑251 (MQ=255)
                                                                            cGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACGTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAggg                                                                                                                                                                                                     <  1:43530/220‑1 (MQ=255)
                                                                             gCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGc                                                                                                                                                                     >  1:316430/1‑251 (MQ=255)
                                                                                 gtgGCGGGCGTGCTAACACCTGTGCGCGGCTAACTGGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGAc                                                                                                                                                                 <  1:265885/251‑1 (MQ=255)
                                                                                   ggCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTc                                                                                                                                                               >  1:250076/1‑251 (MQ=255)
                                                                                   ggCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTAACTGGTTCTGAATCGACTCTTTTCAACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGCCTc                                                                                                                                                               >  1:199592/1‑251 (MQ=255)
                                                                                                     tGTTCGCGGCTACCTTGAGGCCAGGGAACCCGGCTGTATTTCATGATGTTTTCTCCGCCACCAACTTCGCGTACCTGGTTCTGCCTCGACTCTTTTCCACTGGGAAATGGCGGCTTACTGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                                                                                                                                             >  2:151942/1‑251 (MQ=255)
                                                                                                           cgtttAACTTGCGGCCAGGAAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTg                                                                                                                                                                                                                         <  1:225136/165‑1 (MQ=255)
                                                                                                           cgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGACTTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTg                                                                                                                                                                                                                         >  2:225136/1‑169 (MQ=255)
                                                                                                                           aGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCa                                                                                                                        >  1:51884/1‑250 (MQ=255)
                                                                                                                                        ggttttaatgttGTTTGCTCCACCACAAACTTCGAGTACCTGGGTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAAGTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCGTGTTCGGCCTTGTCGGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCAGTGGGAATTTGTTCAATGCTTGTCGCATc                                                                                                          <  2:279408/240‑1 (MQ=255)
                                                                                                                                            ttAAAGATGTTGTCTCCGCCACAAACGTCGAGTTCCTGGTTCTGAAGCGACGCTTTTCCACTGGTAAAGGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGGTTAGCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGGAGGGATTGCGCGGCGGGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACTTAACGCATTGGCAATTTGGGCAAGGCGTGTCGCATCg                                                                                                         <  2:214070/248‑1 (MQ=255)
                                                                                                                                                           ccGCCACAAACTTCGAGTTCCTGGGTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCATGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTGTAtt                                                                                       <  2:226026/251‑1 (MQ=255)
                                                                                                                                                            cGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCAAATCACTTTTATTc                                                                                      >  2:93032/1‑251 (MQ=255)
                                                                                                                                                                    aCTTCGAGTACCTGGTTCTGAATCGACTCTTCTCCACTGGTAAATGGCGTCTTAATGACGAATTGCAGACATACACGTTTTT‑AGGTTTAACAGCATATTGCGCTTTCAATTGCGCGTAATGATCCATAAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGATGACGTAACGGATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGa                                                                               >  1:153730/1‑250 (MQ=255)
                                                                                                                                                                         gAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATGGCGCGTACTGATcc                                                                                                                                                                                                            <  1:124500/120‑1 (MQ=255)
                                                                                                                                                                         gAGTACCTGGTTATGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATcc                                                                                                                                                                                                            >  2:124500/1‑120 (MQ=255)
                                                                                                                                                                                           cGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTc                                                       <  1:121812/251‑1 (MQ=255)
                                                                                                                                                                                             aCTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCgg                                                     >  1:163447/1‑251 (MQ=255)
                                                                                                                                                                                                      cACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTc                                            >  1:157937/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gCAGAAAAACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCGGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGATCGGGTTTCa              <  1:151942/251‑1 (MQ=255)
                                                                                                                                                                                                                                      aGAAACACACGTTTTT‑AGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCACTCACCAGATCGGGTTTCAgc            >  2:93612/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cgtttttAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCTACGCCAGGCAATCACCAGATCGGGTTTCAGCGCGACAATg   >  1:33737/3‑251 (MQ=255)
                                                                                                                                                                                                                                                 gtttttAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGATCGGGTTTCAGCGCGGCAATGc  <  1:93032/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 gtttttAGGTTTAGCAGCATATTGCCCTTTCGATTGCGCGTACTGAGCCAGCAGGGATGGCGCGGCTTGTTCGGCCTTGGCTTGTTGCGGCCTCCAGGGGGCCAGTTGACGTAACTCATTGGCAATTTGTGCAATGCTTGTCGCATTGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCCCCAGATCGGGTTTCATCGCTGCAATGc  <  2:278271/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
TAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGATCGGGTTTCAGCGCGACAATGC  >  NC_000913/177754‑178245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑