Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,144	(T)8→7	intergenic (‑11/+369)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,144	0	T	.	100.0%	105.0 / NA	25	intergenic (‑11/+369)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/11);  total (14/11)

TACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAAT  >  NC_000913/696920‑697380                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             tACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACACCCGCAAAAAAAGATCGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCgggg                                                                                                                                                                                                                    <  1:272636/250‑1 (MQ=255)  aCCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGCCCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGgcg                                                                                                                                                                                                                  >  2:59712/1‑251 (MQ=255)             aTACCCCATCCGTACAACGCTTTCTGGGGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGCTACCTTATCGGCGTTGCGGGGCGCATTATGCGtt                                                                                                                                                                                                       >  2:1521/1‑250 (MQ=255)                               gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTc                                                                                                                                                                                      >  2:312313/1‑250 (MQ=255)                                      ggTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACctc                                                                                                                                                                                <  1:300556/249‑1 (MQ=255)                                               ggCGGGAGGCGAGACTTGAACTCTCACACCTTGCGGCGCCAGAACCAAAATCTGGTGCGTCTACCAATTTCGCCACGCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGATCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGTGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGa                                                                                                                                                                     <  1:265042/250‑1 (MQ=38)                                                     aGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACTGGATTCGAACCTGTGACCCCCTCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGACGTTGCGGAGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTg                                                                                                                                                               >  2:240664/1‑251 (MQ=255)                                                                                       aGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAg                                                                                                                             <  1:312313/251‑1 (MQ=255)                                                                                            cTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATa                                                                                                                                                                                                                                       >  2:300075/1‑140 (MQ=255)                                                                                            cTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATa                                                                                                                                                                                                                                       <  1:300075/140‑1 (MQ=255)                                                                                                    ggggCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCGGTGACCCCATCAGTATGAGTTATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATTCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCTAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTatat                                                                                                                <  1:205444/248‑1 (MQ=39)                                                                                                      tGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGATGGCTACCACGGGCTTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCACGGACAATTGCTCGAACGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCACTATATTc                                                                                                              >  2:142042/1‑251 (MQ=38)                                                                                                                            cccGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTtgtg                                                                                        >  1:41176/1‑251 (MQ=255)                                                                                                                                cAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGtttt                                                                                    <  1:176132/251‑1 (MQ=255)                                                                                                                                 aaaaaaaGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCa                                                                                                                                                                                     >  2:49114/1‑153 (MQ=255)                                                                                                                                    aaaaTATGGTGTCTACGACGTGATTCGAACCTGTGACCCCATCATTATGAGTTATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGTCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAATTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTcc                                                                                <  1:124450/251‑1 (MQ=38)                                                                                                                                          tggtggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACttt                                                                                           <  2:218702/234‑1 (MQ=255)                                                                                                                                          tggtggATACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCCTTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACttt                                                                                           >  1:218702/1‑234 (MQ=255)                                                                                                                                                    gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTTCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTc                                                                <  1:112034/251‑1 (MQ=255)                                                                                                                                                             cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCt                                                                                                                    <  1:65908/190‑1 (MQ=255)                                                                                                                                                             cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCt                                                                                                                    >  2:65908/1‑190 (MQ=255)                                                                                                                                                                                 tATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCAATCTTTTTTT‑CGCGATCCCTTATCGGCGTTGCCGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGACAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAAtt                                   >  1:41706/1‑251 (MQ=255)                                                                                                                                                                                     aGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAAc                               >  1:295421/1‑251 (MQ=255)                                                                                                                                                                                                                 gTAGCCAGCTTTTTTT‑CGCGATACCTTATCGGCGGTGCGGGGCGCCTTATGCGTTTAGCGCCTTGCCGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTGTTTCTAGGTTGCGAACAGCGTGGCGCTACATTCGCAAATTATTGTTTACTTTGTGTTTTTTCCCTCCCTACAGCCATTCTTTTGTCACACAGGATGAAATTCGGCATTTAACCCTAGTGTTGGGCATTTAATCtctgta    >  2:78664/1‑245 (MQ=255)                                                                                                                                                                                                                   aGCCATCTTTTTTT‑CGCGATACCTTATCGGCGTTGCGCGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAACTTGATCGGAAGCGACTGTTTGGTTAGGTTGCGAACAGCACGGCGTTTTATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTTATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAAt  >  1:294072/1‑250 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             TACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAAT  >  NC_000913/696920‑697380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑