Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,410,053	(T)9→8	intergenic (+47/‑80)	rlmB → / → yjfI	23S rRNA 2'‑O‑ribose G2251 methyltransferase/DUF2170 domain‑containing protein YjfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,410,053	0	T	.	100.0%	64.3 / NA	15	intergenic (+47/‑80)	rlmB/yjfI	23S rRNA 2'‑O‑ribose G2251 methyltransferase/DUF2170 domain‑containing protein YjfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (5/10);  total (5/10)

ATCAGAGCAAAATGACCGGACGCCTGGCGCTGGTGATGGGTGCGGAAGGTGAAGGTATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTGATGTAACAAATAGCGAGAACGCATTAATTATTAAAATGAATGATTATGGCGATTTGCAAATCAATATTC  >  NC_000913/4409822‑4410262                                                                                                                                                                                                                                        |                                                                                                                                                                                                                  aTCAGAGCAAAATGACCGGACGCCTGGCGCTGGTGATGGGTGCGGAAGGTGAAGGTATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGCTCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGACCGTTTCGGTTGCGACCGGAATTTGCTTATTTGCAGCGGTGCGCCAGCGCAGCTAATTTCTCAGACTTATGAAAAGCCATCCAGCTTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATa                                                                                                                                                                                                >  1:295299/1‑250 (MQ=255)              gACCGGCCGCCTGGCGCTGGTTATGGGTGCGGAAGGTGAAGGTATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGTACGGAATTTGCTTATGTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCa                                                                                                                                                                                   <  2:229118/250‑1 (MQ=255)                          ggCGCTGGTGATGGGGGCGGAAGGTGAAGGTATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGAACAGGAGCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                                                                                                                                                        <  1:133296/249‑1 (MQ=255)                                                         aTGCGTCGCCTGACTCGTGAACAGTGCGATGAGTTGATCAGCCTCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGTTTGCGAACGGAATTTGCTTATTTGAAGCGGTGCGCCTGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTTTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTGTATTAATATTTAAACTTAATCTATATGGaata                                                                                                                                        <  2:122817/250‑1 (MQ=255)                                                           gCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata                                                                                                                                     <  1:105765/251‑1 (MQ=255)                                                                gCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAATATAATTACGTATTGCAAATATTCATGGTATTAATATTTAAACTTAATCTATATGGAATAATATATGa                                                                                                                                <  1:105635/251‑1 (MQ=255)                                                                                                 gCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAACAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGc                                                                                               >  2:229920/1‑251 (MQ=255)                                                                                                   ataccGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGAAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTgaa                                                                                              >  2:83258/4‑248 (MQ=255)                                                                                                                           tcCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTg                                                                       <  1:44428/249‑1 (MQ=255)                                                                                                                                gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCAATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTGATGTAAc                                                                >  2:196492/1‑251 (MQ=255)                                                                                                                                    gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                                                                                                                                                  >  1:162962/1‑149 (MQ=255)                                                                                                                                    gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                                                                                                                                                  <  2:162962/149‑1 (MQ=255)                                                                                                                                      ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTGATGTAACAAATa                                                           <  1:275300/250‑1 (MQ=255)                                                                                                                                                                               aGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGAGTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATAGTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTGATGTAACAAATAGCGAGAACGCATTAAGTATTAAAATGAATGATTATGGCGAtt                 <  2:77467/251‑1 (MQ=255)                                                                                                                                                                                               cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTT‑GTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTGATGTAACAAATAGCGAGAACGCATTAATTATTAAAATGAATGATTATGGCGATTTGCAAATCAATATTc  <  2:65184/250‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                  ATCAGAGCAAAATGACCGGACGCCTGGCGCTGGTGATGGGTGCGGAAGGTGAAGGTATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATATTGATGTAACAAATAGCGAGAACGCATTAATTATTAAAATGAATGATTATGGCGATTTGCAAATCAATATTC  >  NC_000913/4409822‑4410262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑