Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,318,016 (C)5→4 intergenic (‑10/+197) rimP ← / ← metY ribosome maturation factor RimP/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,318,0160C.94.4% 69.0 / ‑3.1 18intergenic (‑10/+197)rimP/metYribosome maturation factor RimP/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (11/6);  minor base G (0/1);  total (11/7)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GAGACTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCCCAAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATG  >  NC_000913/3317777‑3318255
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
gagaCTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATaaaa                                                                                                                                                                                                                                      >  1:291054/1‑250 (MQ=255)
                   cAACGGTGCTGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATAATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGACAACCAGTTCAAAAACCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGAGGACAAGCCCACCCA‑AAGACATAAAAAAAGGGCCTAAAGCCAAGtt                                                                                                                                                                                                                  >  2:235168/1‑251 (MQ=255)
                           aTGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGt                                                                                                                                                                                                           >  1:171003/1‑250 (MQ=255)
                                                             ggtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCt                                                                                                                                                                        >  1:98345/1‑251 (MQ=255)
                                                                              caATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACaaaa                                                                                                                                                                                            >  2:6869/1‑214 (MQ=255)
                                                                              caATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACaaaa                                                                                                                                                                                            <  1:6869/214‑1 (MQ=255)
                                                                                     caACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCg                                                                                                                                                <  1:188837/251‑1 (MQ=255)
                                                                                                       cACTATCAATATAGATGCGCAGTGTGGATGTCCCACCCCGAATAAATTCAATCCCAACCAGTTCAAAACCCAGGGCCTCACCTGGCGCCGTAATCATCTCTGTTACTTTTTGCTCTAATGTGGACAACACCACCCC‑AAGACATAAAAAAAGGACCTACAGCCCAGCTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACAGCAACTGCGGTCTGGAGc                                                                                                                               >  1:309805/1‑250 (MQ=255)
                                                                                                                              gtgGATGTGCGACCGCGAATTAATTCGATGCAAACCAGTTCAAAACCCAGGGCCGCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAAGGTGGACAAGCCC‑TCCCGAAGGAATAAAAAAAGGGCCTAAAGCCCAGTTATTCGGTAGTCATATAGCAAAAAACCCCTATAGATCGGGCCTTTATATAACTTAACCCTGTAACGGCAGCTGCGGACTGCAGCGCCTGCCAGAAGGATTTTTTCaa                                                                                                        <  2:144495/250‑1 (MQ=255)
                                                                                                                                            gcgAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTGTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTTCGGTCTGGAGCACTGGCCAGAAGGATTTTTTCAAATCCCACTACGAAgg                                                                                         <  2:132222/251‑1 (MQ=255)
                                                                                                                                             cgAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAAGCATCTCTGTTAATTTTTGCTAGAATGTGGACAAGCCCACCCA‑AAGCCATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATA‑CTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACGACGAAGGcc                                                                                       <  2:148986/251‑1 (MQ=255)
                                                                                                                                                    aTTCGATTCCAACGAGTTCAAAACCCAGGGGCTCAGCTAGCGCGATAATCATCTCTGTTAATTTTTACTCTAATGTGAACAAGCCCACCAC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAATCCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCCACTGCAGTCTGAAGAACTTACAAGAGGGATATTTTAAAATCCCAcgaacaaagccgaagac                                                                                 >  2:72599/1‑235 (MQ=255)
                                                                                                                                                           gCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTcaca                                                                           <  2:291054/250‑1 (MQ=255)
                                                                                                                                                                                       aCTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGaa                                               >  2:119568/1‑250 (MQ=255)
                                                                                                                                                                                               aGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGAAAGTGGTTGc                                      >  1:269875/1‑251 (MQ=255)
                                                                                                                                                                                                        ctctGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGAAAGTGGTTGCGGGGGCCgg                             >  2:99070/1‑251 (MQ=255)
                                                                                                                                                                                                               aaTTTTTGATCTAATGTGGACAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGAAAGGGGTTGCGGGGGCCGGATTTGaa                      <  1:198363/251‑1 (MQ=255)
                                                                                                                                                                                                                                   cAAGCCCACCCC‑AAGACATAAAAAAAGGGCCTAAAGCCCAGATATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATg  >  1:269614/1‑251 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
GAGACTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTGCGACCGCGAATAAATTCGATGCCAACCAGTTCAAAACCCAGGGCCTCAACTGGCGCAGTAATCATCTCTGTTAATTTTTGCTCTAATGTGGACAAGCCCACCCCCAAGACATAAAAAAAGGGCCTAAAGCCCAGTTATTCTGTAGTCAGATAACAAAAAACCCCGATAAATCGGGGCTTTATATAACTGAACCCTATAACCGCAACTGCGGTCTGGAGCACTTTCCAGAAGGATTTTTTCAAATCCCACTACGAAGGCCGAAGTCTTCACAGTATATTTGAAAAAGGACTCTAAGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATG  >  NC_000913/3317777‑3318255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑