Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,207,5490G.97.0% 137.8 / ‑3.9 33coding (179/606 nt)ttdBL(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (17/15);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAG  >  NC_000913/3207309‑3207788
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               
ggCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgc                                                                                                                                                                                                                                      >  1:139909/1‑251 (MQ=255)
                                tGAACATCTGTGTCACACCCGGAGCGCGTTAGTAAAAAGATCCTG‑CAACCCCCGATCAAA‑CTGGAAGATGTCAAAGATATTCGCGGCGGGTGTGTGATCTCCTTGACCGGTAAGC‑GGTGGACCTTCCGCGTCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGAGCTCAACGGCAAAGCGATTGTCCACGCTG‑CCCCATCGTGCGCAAAAACGGTGACAAATGGGAGATGGtctc                                                                                                                                                                                                       <  2:73208/250‑1 (MQ=255)
                                    aaTCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCg                                                                                                                                                                                                  >  1:293241/1‑251 (MQ=255)
                                         gTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCg                                                                                                                                                                                             >  1:76625/1‑251 (MQ=255)
                                           cgcacaCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCC‑GAGCAAAGCTG‑AAGATCGGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAc                                                                                                                                                                                           <  1:41313/249‑1 (MQ=255)
                                                   cGGAGCGTGTGAGGAAAAAGATCCTGACATCCCC‑GATCAAAGCTG‑AAGATCGGCAAGATATTCGCGCCGCCGATTTGATCTCCCTGACCGGTACGCTGGGG‑ACCTGCCGCGACGTTTGTCACCGCCGGTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATGTTCCACGC‑GGCCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGAGGTTCTCCGTCGGCCTGACAACCAGTa                                                                                                                                                                                   <  2:35171/251‑1 (MQ=255)
                                                              aTGAAAAAGATCCTGACAACCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCATATGGa                                                                                                                                                                         <  1:87752/250‑1 (MQ=255)
                                                                 aaaaaGATCCTGACACCCCC‑GAACAAAGCTG‑AAGTGCTGCAAGATATCCGGGCCGGCGATGTGATCTCCCTGACCGGTACGCTGGTG‑ACCGCCCGCGACGTTTGTCACCGCCGTTTGACCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGt                                                                                                                                                                     <  2:19951/251‑1 (MQ=255)
                                                                     aGATCCTAACACCCCC‑GATCAAAGCTG‑AGGATCGGTAAGATTTTCGCGGCGGCTAGGTGTTCTCCCTGACCGGGACGCGGGTG‑ACCGGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTTAAACGTCCGAGCCCGTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTg                                                                                                                                                                 <  2:293241/251‑1 (MQ=255)
                                                                            gACAGCCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCTGCGGCGTTGTGATCTACCGGACCGGTACTCTGGTG‑ACCTGCCGCGACTTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTTCGAGCTCAACGGCGAGGCGATGTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAGTGTCGGCGTCGGTCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTg                                                                                                                                                           <  1:146908/250‑1 (MQ=255)
                                                                              cAACCCC‑GATCAAAGCTG‑ACGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGACACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGCATGCGCATGGACAGTTTTGAACGTGAAt                                                                                                                                                        >  2:974/1‑251 (MQ=255)
                                                                               aacacc‑GATCAACGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTCCGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAACCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGCGATGGTCTCCGTCGGCCCGACAACCAGTCTGCGTATGGCAAGTTTTGAACGTGAAtt                                                                                                                                                       >  2:250194/5‑251 (MQ=255)
                                                                                  ccc‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAACAGTATGCGTCTGGAAAGTTTTGTACGTGAATTTAt                                                                                                                                                    >  2:11236/1‑251 (MQ=255)
                                                                                              cTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCg                                                                                                                                                                                                                          <  1:127130/170‑1 (MQ=255)
                                                                                              cTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCg                                                                                                                                                                                                                          >  2:127130/1‑170 (MQ=255)
                                                                                                                      gTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTATCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGACTTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa                                                                                                                   >  2:128989/1‑250 (MQ=255)
                                                                                                                         ggCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCCAAggg                                                                                                               >  2:234349/1‑250 (MQ=255)
                                                                                                                                      aCCTGACCGGTACGCTGGTG‑ACCTGCCTCTACGTTTGTCACCGCCGTTTGAGCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACTGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGGTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATgggg                                                                                                      <  1:292346/247‑1 (MQ=255)
                                                                                                                                                           aCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                >  1:8861/1‑249 (MQ=255)
                                                                                                                                                                      cGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGa                                                                                                                                                 <  1:253552/173‑1 (MQ=255)
                                                                                                                                                                      cGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGa                                                                                                                                                 >  2:253552/1‑173 (MQ=255)
                                                                                                                                                                             cACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATATCAACGGCAACGCGATTTTCCACGCTG‑CCCCATCGCGCGCAACAAAGGCGACAAATGGGAGATGGTCTCCGTCAGCCCGCCAACCAGTATGCGTATGGAAAGTTTTGAACGTGACTTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAt                                                            >  1:298636/1‑251 (MQ=255)
                                                                                                                                                                                 gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAGGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGa                                                        <  1:10727/251‑1 (MQ=255)
                                                                                                                                                                                    gTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAttt                                                     >  1:132996/1‑251 (MQ=255)
                                                                                                                                                                                         aTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCg                                                >  1:62448/1‑251 (MQ=255)
                                                                                                                                                                                                 gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCt                                         <  1:128989/250‑1 (MQ=255)
                                                                                                                                                                                                     cGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGcgcg                                    <  1:215109/251‑1 (MQ=255)
                                                                                                                                                                                                           aTCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTg                              >  1:53006/1‑251 (MQ=255)
                                                                                                                                                                                                           aTCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCACGGCAGGCTGCGCGGTGCTg                              >  1:146437/1‑251 (MQ=255)
                                                                                                                                                                                                                            cGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAACAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGCACGTGAATTTATTGa                                                                                                                                                 >  2:122327/1‑119 (MQ=255)
                                                                                                                                                                                                                            cGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGa                                                                                                                                                 <  1:122327/119‑1 (MQ=255)
                                                                                                                                                                                                                               cAAAGCGATTTTCCACGCTG‑CCACATCGTGCGCAAAAACGGCGACAAATGGGAGACGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAGTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAaga          <  1:260780/251‑1 (MQ=255)
                                                                                                                                                                                                                                        tttCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTgaag  <  2:62448/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               
GGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCC‑GATCAAAGCTG‑AAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAG  >  NC_000913/3207309‑3207788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑