Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,5430CT95.6% 135.8 / ‑1.4 45P79L (CCC→CTT) ppnNnucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (30/13);  total (31/14)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01
*NC_0009132,926,5440CT95.6% 131.8 / ‑1.4 45P79L (CCC→CTT) ppnNnucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (30/13);  total (31/14)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

TTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTA  >  NC_000913/2926308‑2926785
                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                 
ttGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCCGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCAGTACTGAACTACGGAAGTTTGACAGAAAGCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTACCGTCTTGCGCCGTGAACGCGGAGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGAttt                                                                                                                                                                                                                                     >  1:43966/1‑251 (MQ=255)
               aGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCGGGAAGGGGAAATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTGGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAat                                                                                                                                                                                                                      <  1:199291/251‑1 (MQ=255)
                 cccGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAatt                                                                                                                                                                                                                     >  1:167488/1‑250 (MQ=255)
                   cGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCACAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTACAGCTGGAACTGATTAATCTTCCGGAAGAGGCCTTTGCGGATGGGCGAattatt                                                                                                                                                                                                                  >  2:209319/1‑251 (MQ=255)
                     cTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                                                                                                                                                                                >  1:407066/1‑251 (MQ=255)
                     cTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCGTAAACGCACCGCCAGCAGCGCCCTCTATCAACTGTTTCGCAACTGCTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAACAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAATCTGGAACTGATTAATCTTCCGGATGTGGCTTTTGTCGATGGGCGAATTATTcg                                                                                                                                                                                                                >  2:122049/1‑251 (MQ=255)
                                ggatttttgggcgcgtcTGGAAGGGGATATGGTTAACCCCTCCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAgg                                                                                                                                                                                                     <  2:2710/235‑1 (MQ=255)
                                              ggCTGGAAGGGGATATGCTTAACCGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgcg                                                                                                                                                                                       <  1:5080/250‑1 (MQ=255)
                                                       tgtatttgcttaacccaatcGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCTGTACTGAGCTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATGTCGATATTACCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAATTGATTTATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCg                                                                                                                                                                               <  1:397054/231‑1 (MQ=255)
                                                       tGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGt                                                                                                                                                                              >  1:60681/1‑251 (MQ=255)
                                                        ggATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTg                                                                                                                                                                             >  1:396324/1‑251 (MQ=255)
                                                        ccgctcttcctatccccacGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCTAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGTTCCTGCGTg                                                                                                                                                                             <  2:291345/233‑1 (MQ=255)
                                                                       cACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTg                                                                                                                                                                             >  1:291345/1‑236 (MQ=255)
                                                                                              aaCTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACcgtt                                                                                                                                       >  1:459316/1‑251 (MQ=255)
                                                                                                     tCGCAACGGTTCACTGGCCGTACTGATCTCCGGGAGTTTGAACGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCTATAGTAACGTCTTGCTCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGTCTTGTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGGTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGtttt                                                                                                                                 <  2:60681/250‑1 (MQ=255)
                                                                                                       gCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTccc                                                                                                                              >  1:170162/1‑251 (MQ=255)
                                                                                                         aaCTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCa                                                                                                                             >  1:34423/1‑250 (MQ=255)
                                                                                                         aaCTGATCACTTAACGTCCTGCACTCCGGAAGTTTGACCGCTAACAGACAAGAATTGCTGTCTCGTTTTGCAAAGTCCGATATTAACGTATTGCGCCGTGCACACGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTATCGCTTTCCAGGCCAACTTGTTCGCGGTCCTCCCTGTAATTCTCTTCGTTTACGGGCACATCCATacacacatccgttttcccc                                                                                                                             >  2:555122/1‑232 (MQ=255)
                                                                                                                 aCTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGACTTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTATTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAAtc                                                                                                                    >  2:387185/1‑251 (MQ=255)
                                                                                                                         tACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAAc                                                                                                            >  1:158020/1‑251 (MQ=255)
                                                                                                                          aCTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCTTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACt                                                                                                           >  2:376045/1‑251 (MQ=255)
                                                                                                                                  cctgTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCcac                                                                                                   >  1:121383/4‑251 (MQ=255)
                                                                                                                                    ggTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCg                                                                                                                                                                                                                         >  1:351364/1‑131 (MQ=255)
                                                                                                                                    ggTAGTTTGACCGATAACAGCAAAGAATTGCTGTCGCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGTAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCg                                                                                                                                                                                                                         <  2:351364/131‑1 (MQ=255)
                                                                                                                                               cGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACAGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGACAAATCCCTAACACCGTTCGTTATCCCAACCTGAATCTCGACAACTCCGTCCACATCACAAACATgg                                                                                      >  2:501002/1‑251 (MQ=255)
                                                                                                                                                      aGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTc                                                                               >  1:204170/1‑251 (MQ=255)
                                                                                                                                                      aGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGGTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTc                                                                               >  1:395910/1‑251 (MQ=255)
                                                                                                                                                         aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAAtctc                                                                                                                  >  1:95488/1‑213 (MQ=255)
                                                                                                                                                         aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCGTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAAtctc                                                                                                                  <  2:95488/213‑1 (MQ=255)
                                                                                                                                                                   tgtatcGTTTTGACAAGTTCGATATTAACGTCTGGCGCCGTGAACGCGGCGTAAAGCTGTAACTGATTAATCTTCCGGAAGAGGCTTTTGGCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTGTTGCGTGACATGCTCTTCGTTTACGGGCAACTCCATAACACCGTTCGTTTTGCCACCCTGAATCTCGACAACGCCGTCCACATCATTAACCTGGTCTTTTCCATGTTGCGTaa                                                                   <  2:291014/246‑1 (MQ=255)
                                                                                                                                                                       tcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTTACATTCTCTTCGTTTACGGGCAAATCCATAACACcgtt                                                                                                                                       <  1:502223/178‑1 (MQ=255)
                                                                                                                                                                       tcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAATTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAAAACcgtt                                                                                                                                       >  2:502223/1‑178 (MQ=255)
                                                                                                                                                                         gTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCAGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgc                                                            >  1:247891/1‑251 (MQ=255)
                                                                                                                                                                            ttGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgcgcg                                                         >  1:502192/1‑251 (MQ=255)
                                                                                                                                                                                     tCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAtgt                                                 <  1:227972/250‑1 (MQ=255)
                                                                                                                                                                                      cGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATGCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTgg                                               <  1:501002/251‑1 (MQ=255)
                                                                                                                                                                                       gATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTggg                                              <  1:386194/251‑1 (MQ=255)
                                                                                                                                                                                          atTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGCTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCCCGCGCTGCCTGTGGGTg                                            >  2:478603/1‑250 (MQ=255)
                                                                                                                                                                                                             aaCGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTc                        >  1:437734/1‑251 (MQ=255)
                                                                                                                                                                                                                    cgTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGTTGAAGCGCCAAATATAGTGGTCTGCTggg                 >  2:244/1‑251 (MQ=255)
                                                                                                                                                                                                                      tAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTggggg               >  1:287753/1‑250 (MQ=255)
                                                                                                                                                                                                                        aaGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGc                                                                >  2:2484/1‑200 (MQ=255)
                                                                                                                                                                                                                        aaGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGc                                                                <  1:2484/200‑1 (MQ=255)
                                                                                                                                                                                                                              gAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTc       >  1:181146/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aTTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATATCGACAACTCCGTCCCCATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCACATATGGTGGTCTGCTGGGGCGGTCACTCAATTa  >  2:199846/1‑250 (MQ=255)
                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                 
TTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTA  >  NC_000913/2926308‑2926785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑