Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,441,765	C→T	intergenic (‑22/+89)	hslJ ← / ← ldhA	lipoprotein implicated in Novobiocin resistance/D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,441,765	0	C	T	100.0%	88.8 / NA	30	intergenic (‑22/+89)	hslJ/ldhA	lipoprotein implicated in Novobiocin resistance/D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

CCTTCACCGCTAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAG  >  NC_000913/1441523‑1441990                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  ccTTCACCGCTTAAGCGGTTACACATGCTGCCGGAAAGCATCATTTTTCCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCTCTGGCTTACCGTTTATGCTTTCCAGCAAAAAGCGTTGATGCTGTAGCTGTTCTGGCGTAACATCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAATCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAg                                                                                                                                                                                                                           <  1:36743/251‑1 (MQ=255)           tAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGATGATTTCTGGCGGATTTTTATAGCTGGTCACGGGCTTACCGTTTAAGATTTCCAGCAAAAAGAGTTGATGATGTAGCTGTTCTGGCGTACCAGCAATTTTGTAATTACTTACACATCCCGCCATCGGCCGTATTAGCAAAAAAAACGGGGCTACTTTCTTCCTAGTGGTTCGCAATTACGATTTTTGACTCAGGCCTAttttt                                                                                                                                                                                                                 >  2:334498/1‑250 (MQ=255)                  gTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTCCGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGAGGTTCTCAATTACAGTTTTTGAATCAGGACTATTTTAAGAAttg                                                                                                                                                                                                          >  2:151080/1‑249 (MQ=255)                      cacaTGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGTCTATTTTAAGGATAGAgg                                                                                                                                                                                                       >  1:313827/1‑250 (MQ=255)                      cacaTGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAgg                                                                                                                                                                                                       >  2:187040/1‑250 (MQ=255)                                                   aCCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATc                                                                                                                                                                          >  1:8947/1‑250 (MQ=255)                                                         tcTGATTTCTGGCGGATTTTTATCCCTAGTCACGGGCTTACCGTTTACGCTTGCCCGCAAAAAGCGATAATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGAGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGAATGAAAGATCATTGGGGATTATCTGAATCAGcgcac                                                                                                                                                                   >  2:123437/2‑247 (MQ=255)                                                                   ggCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGc                                                                                                                                                          <  1:17683/250‑1 (MQ=255)                                                                    gCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                                                                        <  1:491644/251‑1 (MQ=255)                                                                     gggATTTTTATCGCGGGTCACGGGCTGACCGTTTACGCGTTCCAGCACAAAGCGATGATGCGGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTTCTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                                                                                       <  2:194296/250‑1 (MQ=255)                                                                                    ggTCACGGGCTTTCCGTTTCCGCATTCCAGCACAAAGCGATGACGTTGTAGCTGTTCTGTCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTTGTTCTTAATTACAGTCTTTGACTCAGGACTAGTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                                                                          <  1:511073/249‑1 (MQ=255)                                                                                                aCCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTACCCTGGAATGCAGGGGAGCGGCAAGATTAAACAAgttcgtt                                                                                                                            >  1:101069/1‑251 (MQ=255)                                                                                                 ccgtgtgctcgttccagtccaAAGTGATGATTCTGTGGCGGTTCTGGCTTAACAGCAATTTTGCCTTTTCTTACCCATCCCGCCATCAGCAGGCTTAGCTCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGTGAGCGGCAAGACTAAACCAgttcgttc                                                                                                                           <  2:379079/232‑1 (MQ=255)                                                                                                        cGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                                                                                    <  1:64455/251‑1 (MQ=255)                                                                                                         gatttacAGCACAAAGCGATGATGCTGTAGCTGTTCTGGAGGAACAGCAATTTTGTCATTACTTACACATCGCGACATCAGCAGGATTAGCGCCACATGCGCGGCTACTTTCTTCCTTATGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATTGAGGATGAAAGGTAATTGGGGATTATCTGAATCAGCTCCCCTGGAATGACGGGGAGCGGCAAGATTAAACCTGATCGTTCGGGCCGGt                                                                                                                   >  2:362006/7‑251 (MQ=255)                                                                                                                   acaaatctgtgatgCTGTAGCGGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACTTCCCGCCATCAGCATGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTGCGGGCAGGTTTCGCCtttt                                                                                                         <  2:8947/242‑1 (MQ=255)                                                                                                                         cGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGa                                                                                                   <  1:201636/251‑1 (MQ=255)                                                                                                                           atgatgCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACCCATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCCTTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCCGAtt                                                                                                 >  1:487395/1‑251 (MQ=255)                                                                                                                                gCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTATCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGTAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGACTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCtt                                                                                             >  2:82355/1‑250 (MQ=255)                                                                                                                                         gTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                                                                                     <  1:512038/249‑1 (MQ=255)                                                                                                                                                         aagtttGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAAGTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt                                                                   <  1:435244/248‑1 (MQ=255)                                                                                                                                                            tttGTCATTACTTACACATCCCGCCATCGGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc                                                                 <  1:334498/250‑1 (MQ=255)                                                                                                                                                                             aTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGCATATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTATCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgctg                                               >  2:225328/1‑251 (MQ=255)                                                                                                                                                                               cccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTc                                             >  1:269347/1‑251 (MQ=255)                                                                                                                                                                                  gCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAgg                                          <  1:187040/251‑1 (MQ=255)                                                                                                                                                                                                 aGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGccc                            <  1:52773/250‑1 (MQ=255)                                                                                                                                                                                                      aacaaACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTaaa                      <  2:313827/251‑1 (MQ=255)                                                                                                                                                                                                                   aCTTTCTTAATTGTGGGTCTCAATCACAGGTTTTGACTCAGGACTATTTTAAGACTAGCGGCTGAAAAGACATTGGGGATTATATGAATCAGCTCCCCTGGAAAGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTGCGCCTTTTACCAGATTGCTTAAGTGTTGCAGCGAAGTCTGAGCAATCCTGGTCAGAGCTTCTGCTGTCAGGAGTGCCTGGAGCACGGTaacacgaacgttgtgg         >  2:253576/1‑237 (MQ=255)                                                                                                                                                                                                                     tttCTTCATTGTGGAGCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCCGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTggc        >  1:530083/1‑250 (MQ=255)                                                                                                                                                                                                                           cATTGTGGTTCTCAATTACAGTTTTTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGgcaggcag  <  1:82355/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  CCTTCACCGCTAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACAGCAATTTTGTCATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAG  >  NC_000913/1441523‑1441990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑