Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,569	C→T	R277W (CGG→TGG)	abgR →	putative LysR‑type DNA‑binding transcriptional regulator AbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,569	0	C	T	100.0%	65.5 / NA	23	R277W (CGG→TGG)	abgR	putative LysR‑type DNA‑binding transcriptional regulator AbgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/9);  total (14/9)

GGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACA  >  NC_000913/1405324‑1405802                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ggACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAgtggcg                                                                                                                                                                                                                                       <  1:468247/250‑1 (MQ=255)        gCCGACGCCACACGGCAGCTACTACAATCAGTTGAGTGAATTTCTGGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCGCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTGCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTACTTGATTCAGTGGCGTGATAGta                                                                                                                                                                                                                               <  2:203429/251‑2 (MQ=255)         ccGAAGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCg                                                                                                                                                                                                                              <  1:41772/251‑1 (MQ=255)              gCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGcc                                                                                                                                                                                                                            >  1:262637/1‑248 (MQ=255)                 ccacGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGAGCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGacac                                                                                                                                                                                                                      <  2:106013/250‑1 (MQ=255)                                               aTTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTcc                                                                                                                                                                                        >  1:511053/1‑251 (MQ=255)                                                  gCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCgg                                                                                                                                                                                      <  1:562177/250‑1 (MQ=255)                                                         gATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGGATCAGTCTGGTGGCCAAAAACGACTTTCTCAGCAAACTGCCTGCAGAAATGGGCTGCGATCCCTTGCACGGACAGCGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGGCATTAATCACGCAATTCCGGAGCGAATg                                                                                                                                                                              >  1:538589/1‑251 (MQ=255)                                                                cgcAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTAt                                                                                                                                                                       >  1:265808/1‑251 (MQ=255)                                                                                               gaCGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAAt                                                                                                                                           >  1:411750/1‑248 (MQ=255)                                                                                                    tctcAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCt                                                                                                                                    <  1:134152/250‑1 (MQ=255)                                                                                                        aGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGACGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCATATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCCTTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAAATGCATAAAAAAATAGAGTCTGTCg                                                                                                                                >  1:64784/1‑250 (MQ=255)                                                                                                           ctggaTCAGGCTGGAGGAAAAAAGCGATTTTCTCCGCAAAATGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTTCTGTTGCCGGTTAGCGAAATTTTACCGCAAGCGGCCTATTATTTGATTCAGTGGCGTGATAATCGCCAGCCCCCAATGACAGCGTCATTAATCAACCAATTCCGTCGAGAATGAGGCTATCTGCAAAGTTAAACCTGCATaaaaacaatgagtctataaacat                                                                                                                            >  2:348657/5‑233 (MQ=255)                                                                                                                       ggtggCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGActct                                                                                                                <  2:400875/251‑1 (MQ=255)                                                                                                                         tggCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCACAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGCCATCCGCCAGACTCTAc                                                                                                              >  1:281522/1‑251 (MQ=255)                                                                                                                                   cGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCCGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACCGTacaaaa                                                                                                     >  2:15823/1‑247 (MQ=255)                                                                                                                                            cAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCGTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGGCGACATCCGCCAGACTCTACAGTACACACAGCAGTGc                                                                                             <  2:265808/249‑1 (MQ=255)                                                                                                                                                                  ggCTGCGATCCCTTGCACGGACAGGGGCTGGTTATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCCTTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAAGGTATGTCGACATCCGCCAGACTCTACCGTACACCCAGCAGGGCCTCCTCGTCTTAAATCCGGCAttt                                                                     >  2:310913/1‑250 (MQ=255)                                                                                                                                                                        gATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGATTGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACAAACAACAGTGCATTCACTTCTTAAATCCTGCCTTGTcacct                                                               >  2:194505/1‑251 (MQ=255)                                                                                                                                                                              ttGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGAACGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTgcgc                                                         >  1:225270/1‑251 (MQ=255)                                                                                                                                                                                                                  gAAATTTTACCGCAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCACTTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTACATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGAGTCTTAAATCCGGCATTGTCTCCTATGCGCCGGTGACTGGGTTATAACGGTTTTAACCTTTAGTTg                     >  2:487872/1‑251 (MQ=255)                                                                                                                                                                                                                       tttACCGAAAGCTGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACTGTTTTAACCTTTAGTTGCCaa                 <  2:262725/250‑1 (MQ=255)                                                                                                                                                                                                                            cGAAAGCGGCCTATTATTTGCTTCAGTGGAGTGATAGTCGCCTGAAACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACCTCCGCCAGACTCTACAGTACACACAGCCGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCACGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTttct           >  2:264903/1‑251 (MQ=255)                                                                                                                                                                                                                                     ccTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACa  <  1:537667/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          GGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACA  >  NC_000913/1405324‑1405802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑