Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,069	C→T	G153D (GGC→GAC)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,069	0	C	T	97.6%	124.4 / NA	42	G153D (GGC→GAC)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/18);  minor base G (0/1);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGC  >  NC_000913/1398839‑1399312                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aTTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGAAGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCCCGGCATTTTTCTTCGCCAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCtcatc                                                                                                                                                                                                                                  >  2:34244/1‑251 (MQ=255)                  aGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGACCGTCTTTGATTTCACCGCTCATCAGACGCATCATctgctg                                                                                                                                                                                                                 >  2:475913/1‑251 (MQ=255)                     aTGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACg                                                                                                                                                                                                              >  1:155326/1‑251 (MQ=255)                       gCCGCTTTTCTGGAAGCGACACAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCa                                                                                                                                                                                                            <  1:539115/251‑1 (MQ=255)                                                agACGGCTGATGGTTTCTACCGTCAGGCCCAGCTAGTTACCGGTATCGACCCGAGTCATCGTCAGGCGGAATTCACGAGTGGAGAAGCCGCGTTGGGCCAAACGACGGGACAGGTTGTCGATGAATGAAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCCGGATCCTGGCCTTATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGAAGCAAATTCGGCATTTTGCCGGACAAATc                                                                                                                                                                                   >  2:43957/1‑251 (MQ=255)                                                 gACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCg                                                                                                                                                                                  <  1:34244/251‑1 (MQ=255)                                                          tGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCTTTTTACCGGACAAATCGTCCAGCGtt                                                                                                                                                                         >  1:43697/1‑251 (MQ=255)                                                                 tcccGTCCGGCCCAGATAGGTACCGATATCGCCACGAGGCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACATGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCAGCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGttt                                                                                                                                                                        <  2:371430/243‑1 (MQ=255)                                                                 tACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGttt                                                                                                                                                                        >  1:371430/1‑245 (MQ=255)                                                                    cGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGa                                                                                                                                                               <  1:298718/251‑1 (MQ=255)                                                                        aGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTc                                                                                                                                                           <  1:420026/251‑1 (MQ=255)                                                                              aaatatttACCCATATCGCCACCACTCATCGTCATGCGGAATTCACGAGGGGATAAGCCGCCTTGGGCAAAACGACTGTACATGTTGTAGATAAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAACAACATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTTCTGACTCATATTCGTCATTTTACCGGACAAATCCTCCATCGTTTCGAACGTGATTTCACA‑TAc                                                                                                                                                    <  2:252516/245‑1 (MQ=255)                                                                                 tAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCg                                                                                                                                                                           >  1:52934/1‑226 (MQ=255)                                                                                       ccGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGt                                                                                                                                           >  1:567195/1‑251 (MQ=255)                                                                                          atatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTc                                                                                                                                        >  1:98730/1‑251 (MQ=255)                                                                                            atCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTcc                                                                                                                                       <  1:351636/250‑1 (MQ=255)                                                                                            atCGCCACGAGTCAGCGTCAGGCGGAATGCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACTGGACAGGTTGTAGATGAATGCAGCCATACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGTCGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCa                                                                                                                                      <  2:387578/251‑1 (MQ=255)                                                                                                  aCGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCt                                                                                                                                >  1:430701/1‑251 (MQ=255)                                                                                                  aCGAGTCAACGTCCGGCGGAATTTTCGAGGGGAGAAACCGCGTTTGGCAAAACGCTGGGACAGTTGGTAGTGGATTGCATCCAGACGTTCCTCGTTATTTGTCGTCGACAACAGCAGTATCATGTCCTGAGCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCATCGTGTCGAACGGGGTTTCACA‑GACCATCGAGGTTTCCAGCGCCt                                                                                                                                <  2:501726/251‑1 (MQ=255)                                                                                                       tCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGCTTTCACA‑TACCATCGAGGTTTCCAGCGCCTGcgcg                                                                                                                           >  1:58662/1‑251 (MQ=255)                                                                                                           cGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTGCCAGCGCCTGCGCGAAg                                                                                                                        <  1:159644/250‑1 (MQ=255)                                                                                                               aGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCCGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCgg                                                                                                                   >  2:262933/1‑251 (MQ=255)                                                                                                                     aaTTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATg                                                                                                             >  2:223010/1‑251 (MQ=255)                                                                                                                                   gaAGCCGCGTTGGTCAAAACGCCGGGACAGGCTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGACCAGCGTTTCGAACGGGCTTTCACA‑TACCATCGAGGTTTCCAGCGCATGCGCGAAGATCGGGTGATAGCCGTTACCGATgg                                                                                               >  2:116344/1‑251 (MQ=255)                                                                                                                                       ccGCGTTGGGAAAAACGACGGGACAGTTTGTAGATGAATGCAGCCAGACGTTCCGCGGCATTTTTCTTCGACAACAGCAGGATCATGGCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGACTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                           <  1:403799/251‑1 (MQ=255)                                                                                                                                             tGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATcc                                                                                     >  1:115983/1‑251 (MQ=255)                                                                                                                                              gggCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATccc                                                                                    >  1:414340/1‑251 (MQ=255)                                                                                                                                                  aaaaCGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCTGCATTTTTCTTCGACAACAGCAGTATCATGTCCTGATCGGCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCCGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGACCGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGTGTCAAATCccacca                                                                                <  2:361031/251‑1 (MQ=255)                                                                                                                                                                    gTAGATGAAGGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATgg                                                               <  2:173776/250‑1 (MQ=255)                                                                                                                                                                     tAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGCTCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGCCGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGa                                                              >  1:74307/1‑250 (MQ=255)                                                                                                                                                                            aTGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTg                                                      <  1:223010/251‑1 (MQ=255)                                                                                                                                                                                aGCCAGTCGTACCTCGGCATTTTTCTACTACAACAGCAGGATCATGTCCTGAGCGTCTGTGATTTCACCGCTCAACAGACGCATCAACTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTATCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGTTTATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGAttt                                                  <  2:212797/251‑1 (MQ=255)                                                                                                                                                                                     gACGTTCCTCGGACTTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGAGATTTGAtcg                                             >  1:110801/1‑251 (MQ=255)                                                                                                                                                                                        gTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACAGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATTGTCCCGCGTTTCGAACGGGCTTTCAAA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCCCCAGGACGCCTGCTAAATGGAAAACAGGGATTAGCtcgtcc                                          >  2:38318/1‑250 (MQ=255)                                                                                                                                                                                         ttCCTCGGCATTTTTCTTCGACAACCGCAGGATCTTGTCCTGCTCGTCTTTGATTTCACCGCTCATCAGACGCATCCTCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCATCCAGCGTTACGGACGGGCTTT‑ACAATACCATCGAGGATTCCAGCGACTGCGCGCAGCTCGGGTGATGTCCCATGCACCTGCCGTCAATTCCAACCAGGTCGCCTGCTAAATAGACACCAgataatcactcgtccc                                         >  2:535592/1‑236 (MQ=255)                                                                                                                                                                                           ccTCGGCAATTGTCTCCGACAACAGCAGGATTATGGCCTGAACGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGTCGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCGCTTCGCCt                                       <  2:43697/251‑1 (MQ=255)                                                                                                                                                                                            cccGGCATTTTCCTTCGACAACAGCTGGATCATTTCCTGAGCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TTCCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATTGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCtt                                      <  2:102750/249‑1 (MQ=255)                                                                                                                                                                                            cTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCtt                                      >  1:490028/1‑251 (MQ=255)                                                                                                                                                                                               ggcattttgtgtcGACAACAGCAGGATCATGTCCTGATCGTCTTTGATGTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTGCCGGACAAATCGTCCAGCGTTTAGAACGGGATTTCTCA‑GACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAGATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCTTCGCCTTGCt                                   <  2:263976/240‑1 (MQ=255)                                                                                                                                                                                                            gACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGtat                       >  1:242493/1‑250 (MQ=255)                                                                                                                                                                                                                               ccTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAg   <  1:43957/251‑1 (MQ=255)                                                                                                                                                                                                                                cTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGc  >  1:168871/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACA‑TACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGC  >  NC_000913/1398839‑1399312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑