Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,387,908 C→T P397S (CCG→TCG)  tyrR → DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,387,9080CT95.8% 157.0 / NA 48P397S (CCG→TCG) tyrRDNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/26);  minor base G (0/2);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCG  >  NC_000913/1387663‑1388152
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
ttAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGaa                                                                                                                                                                                                                                                  >  2:275466/1‑250 (MQ=255)
   atgatgGCACTTTCAGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTg                                                                                                                                                                                                                                              >  1:439559/1‑251 (MQ=255)
         gctcTTTCCGTAGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGACCTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCTAAACTGGCCGCt                                                                                                                                                                                                                                        <  1:143075/248‑1 (MQ=255)
                    ggggTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGGGCCGCGTTCGAAACTGGCCGCTGACCTGAATAc                                                                                                                                                                                                                             <  1:226649/250‑1 (MQ=255)
                          ggCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACt                                                                                                                                                                                                                       >  1:511995/1‑251 (MQ=255)
                              aggcccATGAGGTGCATGTCGAGGTTCGGGTGATTTGCGCTACGCAGAAGAATCTGGCCGAACTGGTGCAAAAAGGCATGTCCCGTAAAGATCTCTATTACCGCCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTAcg                                                                                                                                                                                                                   <  2:111501/247‑1 (MQ=255)
                                gACCATGAGGTGCATGTCGATGTGCGGGTGATTTTCGCTACGCAGCAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGGGAAGATCTCTATTATCGTCTGACCGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGAAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAGACTGGCCGCTGACCTGAATAcgttaattacacg                                                                                                                                                                                                                 >  2:13134/1‑239 (MQ=255)
                                    aTGAGGTGCATGTCGAGGTGCGGGGGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTAGCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTAt                                                                                                                                                                                                             <  1:229288/251‑1 (MQ=255)
                                          tGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTgg                                                                                                                                                                                                       >  2:167011/1‑251 (MQ=255)
                                            cATGTCGATGTTCGGGTGATTGGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCGGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGcc                                                                                                                                                                                                     <  1:26027/251‑1 (MQ=255)
                                                       gCGGGTGATTCGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTTTCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGc                                                                                                                                                                                          <  1:390488/251‑1 (MQ=255)
                                                         gggTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATAATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGCCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTCCTTACGCGTTATGCGTGGCCGGGAAATGTGCgg                                                                                                                                                                                        >  2:103707/1‑251 (MQ=255)
                                                          ggTGATTTGCGCTACGCAGAAGAATCTGGTCGCACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTAATTACGCGTTATGCGTGGCCGGGAAATGTGCGGc                                                                                                                                                                                       >  2:197912/1‑251 (MQ=255)
                                                                     cTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAAc                                                                                                                                                                            >  1:281412/1‑251 (MQ=255)
                                                                        cGCAGAAGAATCTGGTCGAACTGATGCAAAAAGGCATGTTCCGTGAAGAACTCTATTATCGACTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACGGTCCGCAGGACATCATGCCATTAACTGAGCTGTTCATCGCCCGCTTTGACGACGAGCAGGGCGTGCCACGTTCGAAACTGGCCGCTGTCCTGAATACTGGACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGcc                                                                                                                                                                         >  2:512216/1‑250 (MQ=255)
                                                                          cAGAATAATCTGGTCGAACTGGTGCAAAAAGGCCTGTTCCGTGAAGATCTTTTTTATCGTCTGAACGTGTTGACGCTCATCCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGctat                                                                                                                                                                       <  1:166081/251‑1 (MQ=255)
                                                                                    tGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCgcgc                                                                                                                                                              >  1:322988/1‑250 (MQ=255)
                                                                                     ggTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATcgc                                                                                                                                                                <  1:167011/247‑1 (MQ=255)
                                                                                     ggTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGGGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCACGCAc                                                                                                                                                            >  2:431403/1‑251 (MQ=255)
                                                                                                 gtaaacaGGCATGGTCCGTGATGATCTGTATTGTCGTCTGCCGGCGTTGACGCTAAATCTGCCGCCGCTACGTGACTGTCCGCAGATCATCATGCCGTTAACTGATCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTGCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGATCGCTATCTATCGCGCATTGACACAACTgg                                                                                                                                                <  2:266928/245‑1 (MQ=255)
                                                                                                    aaaaGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCCATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACg                                                                                                                                             >  1:349098/1‑251 (MQ=255)
                                                                                                       aGGCATGTTCCGTGAAGATCTCTATTACCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTCCGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGtt                                                                                                                                          <  1:169414/251‑1 (MQ=255)
                                                                                                        ggCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTa                                                                                                                                         <  1:262923/251‑1 (MQ=255)
                                                                                                          cATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATg                                                                                                                                       <  1:181952/251‑1 (MQ=255)
                                                                                                              ccgagctaaGATCTCTATGATCGCCTGAGCGTTTTGCCGCCCAGTCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCt                                                                                                                                   <  2:112052/244‑1 (MQ=255)
                                                                                                                  gTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGt                                                                                                                               >  1:130688/1‑251 (MQ=255)
                                                                                                                   ggAAGATCTCTATTTGCGTCTGAACGTGTTGACGCTCAATCGGCCGCCGCTACGGGACGGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTc                                                                                                                              <  1:471810/250‑1 (MQ=255)
                                                                                                                     aaGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCt                                                                                                                                   >  1:112052/1‑244 (MQ=255)
                                                                                                                        aTCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCcac                                                                                                                           >  1:74641/1‑249 (MQ=255)
                                                                                                                         ccgctattctctgctgtagtgtttGACGATCAATTTGCCGCTGCTACGTGACTGTCCGCAGGACACTATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTGCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGGGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAgg                                                                                                                        <  2:46737/229‑1 (MQ=255)
                                                                                                                                 atcttCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATAtttt                                                                                                                 <  1:59667/246‑1 (MQ=255)
                                                                                                                                        gAACGTGTTGACGCTCAATCAGCCGCCGCTACGTGACTGTTCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAATTAAAGAACGCTATCTAGCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGc                                                                                                            <  1:325497/248‑1 (MQ=255)
                                                                                                                                           cGTGTTGACGCCCAATCGGCCGCCGCTCCGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGAtt                                                                                                      <  1:275466/251‑1 (MQ=255)
                                                                                                                                            gtgtTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGAtt                                                                                                      >  1:474908/1‑250 (MQ=255)
                                                                                                                                                            cgccctgccTACGTGACTTTCCGCAGGACACCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGTCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGGGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGTACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTa                                                                                     <  2:169582/243‑1 (MQ=255)
                                                                                                                                                               cgccgcTACGTGACTGTCCGCGGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGcc                                                                                  <  2:520044/251‑1 (MQ=255)
                                                                                                                                                                        gTGACTGACCGCAGGACATCCTGCCGATAACTGAACTGTTCGACGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTTTGAGCTGCGTCCACAGGATATTTTGTTGCCGGTTTAAGACGCCGCAACGGTAGCCGTGGGCgt                                                                          >  2:384560/1‑249 (MQ=255)
                                                                                                                                                                                                        gAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCTCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATctc                                         <  2:373340/251‑3 (MQ=255)
                                                                                                                                                                                                          gCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCa                                       <  1:244832/251‑1 (MQ=255)
                                                                                                                                                                                                            tGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGGATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTCTCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGAAGCCGCGACGGGAGCCGTGGGCGAAGATGGGATGGAAGGTTCGCTGGACGAAATCACCAGc                                     >  1:163770/1‑251 (MQ=255)
                                                                                                                                                                                                                                  cgaGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGAGGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTaa               <  1:471071/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCTCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATAGTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAAcc             <  1:42588/251‑1 (MQ=255)
                                                                                                                                                                                                                                       aGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTTTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGTTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAg          <  1:194483/251‑1 (MQ=255)
                                                                                                                                                                                                                                          gCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGCTGGAAGGTTCGCTg                                                    >  2:465512/1‑206 (MQ=255)
                                                                                                                                                                                                                                          gCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTg                                                    <  1:465512/206‑1 (MQ=255)
                                                                                                                                                                                                                                            gTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGAtt                                                                                                      >  1:402664/1‑154 (MQ=255)
                                                                                                                                                                                                                                            gTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGAtt                                                                                                      <  2:402664/154‑1 (MQ=255)
                                                                                                                                                                                                                                               ccGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTAGCGCGCACTGACCCAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCCTTTTGAACGCTCGGTATTAACCCAGCTGTATct  <  1:392527/251‑2 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
TTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCG  >  NC_000913/1387663‑1388152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑