Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,384,652	A→G	D179G (GAC→GGC)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,384,652	0	A	G	100.0%	72.0 / NA	25	D179G (GAC→GGC)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/14);  total (11/14)

CCAACGCGCGGCGTCAGTGAAATCCGCCTGGCGCTACGTTTTAAATCGAATGATTCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATA  >  NC_000913/1384408‑1384867                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                        ccaacgcGCGGCGTCAGTGAAATCCGCCTGGCGCTGCTTTTTAAATCGAATGATTCGCTGTTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGTTTGCTCGCCCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATTACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCt                                                                                                                                                                                                                   <  1:252674/251‑1 (MQ=255)   aagccgcgGCGGCAGTGAAAACCGCCTGGCGCTGCGTTTGAAATCGAATGATGCGCTGCTACGCCACTTTAAGGATACCTCCGCGCTGTATCGGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAg                                                                                                                                                                                                                 <  2:209686/247‑1 (MQ=255)                                      gTTTTAAATCGAATGATTCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCACGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTCAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGACGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTgcc                                                                                                                                                                              >  1:373960/1‑251 (MQ=255)                                                  aTGATTCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGAcc                                                                                                                                                                  <  1:236573/251‑1 (MQ=255)                                                    gATTCGCTGCTACGCCACTTTAAGGATACCTCCCCGCTGTATCTGGAAATTGTGGATTATCCCGGCGATTGGTTGCTCTACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCg                                                                                                                                                                 <  1:529389/250‑1 (MQ=255)                                                         gctgctACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTAtc                                                                                                                                                           >  1:534938/1‑251 (MQ=255)                                                          ctgctACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGCGAATGGTCGGCGAAATGGCGACTGATGAGAGAAGGCCGGGGCCCGCCAGCGCCTGCCGACGAAAACCGGCGGGCGGAAATTGCCGCCGCGGGGCCCAATTAtcc                                                                                                                                                          >  2:433754/1‑250 (MQ=255)                                                                cGCCTCTTTAAGGATCCCTCCCCGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTccacca                                                                                                                                                    <  1:303414/251‑1 (MQ=255)                                                                  ccACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACt                                                                                                                                                  <  1:135555/251‑1 (MQ=255)                                                                   cgttttAAGGATACCCCCACGCTTTGTCTGGAAATTGTGGATTACCCTGACGATTGGTGGCTCGCCCTGACGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGGCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCGCCACTg                                                                                                                                                 <  1:100750/248‑1 (MQ=255)                                                                           ggttACCTCCCCGCTGTATCTGGAAATGGTGGTTTATCCCGGCGAATGGTGGCTCGCCCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGc                                                                                                                                         <  1:9631/248‑1 (MQ=255)                                                                               aCCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAggg                                                                                                                                     >  1:401513/1‑251 (MQ=255)                                                                                         tGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGGGCAGGGGCTGCACttt                                                                                                                           >  1:188110/1‑251 (MQ=255)                                                                                                  aaatgggggggtATCCCGGCGAGTGGGTGCCGGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCt                                                                                                                  <  2:401513/240‑1 (MQ=255)                                                                                                      tgtgGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGCTGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGCAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGCTTCTCTTCACCACGGTAAAGCGGAGGGGGTGccatttcttccaccagggc                                                                                                              >  2:47729/1‑233 (MQ=255)                                                                                                           aTTATCCCGGCGAATGTTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGTGGCTGCACTTTATTCAGCCTGGGCGCttt                                                                                                         <  1:436149/251‑1 (MQ=255)                                                                                                            ttATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTg                                                                                                        >  1:421031/1‑251 (MQ=255)                                                                                                                 ccGGCGAATGGTTGCGCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACAGGCTTGCGGGCATTGCCGCCGAGTGGGCCGAGTATCTCCACCACTGAGAAGAGCAGGGGCTGGACCTTTTTCAGCCTGGGCGCttgtgtttt                                                                                                   >  2:351037/1‑244 (MQ=255)                                                                                                                                         cGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGccc                                                                           <  1:256004/251‑1 (MQ=255)                                                                                                                                         cGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAACGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGccc                                                                           >  1:305286/1‑251 (MQ=255)                                                                                                                                                                                 gACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAAGTGCGAATGAGGAGCTAAGGGCCGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGTGTGTACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGTTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTggg                                   <  2:352260/251‑1 (MQ=255)                                                                                                                                                                                                    cAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGCGATATGGCAGGGGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGGGAGTCCAAACTGgcgc                 >  1:484434/1‑250 (MQ=255)                                                                                                                                                                                                                  gTCGGCGAAATGGCGAATGCTGAGCGAAGGGCTGGGTCCGCTAGCGCCGGCTGACGAAAACCGGCTGGCGGACATGGCCGGCGCGTGGACCGATTATCTCCACCACAGTAAAGAGCAGGGGCTGCACTTTATTCAGACTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTGCCCGTGGCCGGATGTCGATACCTGGTGCGAGGCCAAACTGGCGCAGGCCGATAAGCATa  <  2:248785/251‑1 (MQ=255)                                                                                                                                                                                                                       cGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCCCTGTAAAGAGCAGGGGCTg                                                                                                                                 >  2:331860/1‑119 (MQ=255)                                                                                                                                                                                                                       cGAAATGGCGAATGATGAGCGAAGGGCTGGGCCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTg                                                                                                                                 <  1:331860/119‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                        CCAACGCGCGGCGTCAGTGAAATCCGCCTGGCGCTACGTTTTAAATCGAATGATTCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATA  >  NC_000913/1384408‑1384867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑