Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,759	G→A	A63V (GCT→GTT)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,759	0	G	A	100.0%	109.6 / NA	35	A63V (GCT→GTT)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

AACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAA‑GCCCGCTCATTAGGCGGGCTGGGTATCTG  >  NC_000913/1322513‑1323004                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        aaCATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGt                                                                                                                                                                                                                                                     <  1:511676/251‑1 (MQ=255) aaCATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGt                                                                                                                                                                                                                                                     >  1:168868/1‑251 (MQ=255)    aTGGCTTCTCGTTCTTCCTTCGGTACATGCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACTTAAAACTGTAAt                                                                                                                                                                                                                                                  <  1:410933/251‑1 (MQ=255)      ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATg                                                                                                                                                                                                                                                 <  1:238250/250‑1 (MQ=255)                            aatttcgacagcatCTGCAAACAACGAAAGGCGGCAAAAACCGAAAGGGGGCATAAGCGGTCGTCTTCATCCAGCATTGGGCTGACAGGTGGGACGCGCAGCACACTGCTGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGTCGTTATCCAGTAGTGCCAGGAGGGTTTCGCCGGTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACGTAAAACTGTAATGCGCAGCGCACTGTCTACcagca                                                                                                                                                                                                                           <  2:292781/237‑1 (MQ=255)                             cATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACcagcagc                                                                                                                                                                                                                         >  1:520249/1‑251 (MQ=255)                                     aGATTCTGCAATAAACGGAAAGCGTCAAAAACCTAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTTCCAGCAGCAGGC‑tttt                                                                                                                                                                                                                <  1:124958/251‑1 (MQ=255)                                                   aCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGc                                                                                                                                                                                                  <  1:306911/251‑1 (MQ=255)                                                    cggcaaGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCTTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCAATTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGTCAGTGCCTGGAATGTGCCAGTGCCACCTAAAACTGTAATGCGCGGCACACTGGCTACCAGCAGCAGGC‑TTTTTAAATCATCATTGCt                                                                                                                                                                                                 >  2:162416/5‑251 (MQ=255)                                                     ggAAAGCGTCAAAAACCGAACGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACAGCCAGGGGGGAAGCGCAGCACAAGGCAGATTGGTGAGTGTTCACTTTCCACACCCGCAGGCCGGGCGTTATCCAGTAGTGCACGGAGGGCGTCGCCGGTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCGACAACTGTCATGCTCAGCGCACTGTCTAACAACAGCAGGCATTTTTaatactggttgctg                                                                                                                                                                                                 >  2:187499/1‑239 (MQ=255)                                                                  aaCCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCgg                                                                                                                                                                                   <  2:198567/251‑1 (MQ=255)                                                                        aaGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTcc                                                                                                                                                                              >  1:330053/1‑250 (MQ=255)                                                                              gCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCcagcagc                                                                                                                                                                        >  1:339716/1‑250 (MQ=255)                                                                                       ggCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCg                                                                                                                                                              >  1:155431/1‑251 (MQ=255)                                                                                       ggCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑CTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCg                                                                                                                                                              <  1:162416/251‑1 (MQ=255)                                                                                             ttCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGAt                                                                                                                                                        >  1:168096/1‑251 (MQ=255)                                                                                             ttCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGGTTCACCTAAAACTGAAATGCGCAACGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGATTCCGGACGAt                                                                                                                                                        >  2:484002/1‑251 (MQ=255)                                                                                                  ccAGCAGTGGATTGACAGGTGGGAAGAGCAGCACCCTGCAGTTTGGTGAAGGTTCACTGTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCTGGAGGGCTTCGCCGTGGCCGGATAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCca                                                                                                                                                   <  2:128013/251‑1 (MQ=255)                                                                                                       agTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAAACATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCcacaca                                                                                                                                               >  2:117932/1‑250 (MQ=255)                                                                                                         tGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACt                                                                                                                                            >  1:558951/1‑251 (MQ=255)                                                                                                           gACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTgg                                                                                                                                          >  1:11145/1‑251 (MQ=255)                                                                                                                 cAGGGGGGAAGAACAGCACACGGCAGTTTGGTGATTGTTCACTTTCCCCACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCAATGTCTACCAGCAGCCGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCAACACAACTGGTGaaa                                                                                                                                     >  2:557194/1‑250 (MQ=255)                                                                                                                                                            ttCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTTAAAAAGCGCGGTTGTATTGTCGCGATAAGCGCCTTCGCAGTTTAGc                                                                                          <  1:151098/250‑1 (MQ=255)                                                                                                                                                              ccACACCCGCAGGCAGGGCGTTATCCAGTAGGGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAg                                                                                        <  2:11145/250‑1 (MQ=255)                                                                                                                                                                aaacCCGCTGGCAAGTCGTTATCCTGTAGTGCCAGGAGGGCTTCGCCGTTGTCGGAAAGTGTCTGTATTGTGACAGTTTTACTTATAACTTTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGAGATCTGCGGATTCCAGCAGCAGCTTTGCCGGACGATCCCCACACAACTTGGGAAAAAGCGCGGTGGGATTGTCGCGATATGCGCCTTCGCAGGTTAGCAGTTc                                                                                     <  2:336267/249‑1 (MQ=255)                                                                                                                                                                        gggcaggGGTTTATCCAATTGTGCCAGGAGGGCTTCGCCGGTGCCGGTAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCTCAGCGCACTATCTACCAGCAGCAGGC‑GTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCTTTGCCGGTCGATCCCCCCACAACTGGTGAAAAAGCGCGGTGGGATTGGCGCGATAAGCGCCTTCGCAGGTTATCAGTTCGTGAGTca                                                                             <  2:89159/250‑2 (MQ=255)                                                                                                                                                                                cGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAgcg                                                                                                         <  1:259903/215‑1 (MQ=255)                                                                                                                                                                                cGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTtgt                                                                     <  1:117932/251‑1 (MQ=255)                                                                                                                                                                                cGTTATCCAGTAGTGCCAGGAAGGCTTCGCCGTTGCCGGAAAGTGCCTGGCTTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAgcg                                                                                                         >  2:259903/1‑215 (MQ=255)                                                                                                                                                                                               ccagtaggttgtCGCCGTGGCCTGAAAGTGCCTGGATTGGGTCAGGGTCACCTAAAACTGTAATGCGCAGCGCACTGCCTACCAGCAGCAGGC‑TTTTTAAATCATCGTTGCGGTCGATATCGGCGGATTCCAGGAGCAGCGTTGCCGGACGATCCCCACACATCTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCGTCGTAGGTTAGCAGGGCGACAGTCGGGGTTTGTGTGTGCGGTGTTAtt                                                      <  2:291554/240‑1 (MQ=255)                                                                                                                                                                                                     gggCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGGTTTTGTGTTTGCATTGTTTTTCTCTa                                                 >  2:134270/1‑250 (MQ=255)                                                                                                                                                                                                               gTTGCCGGAAAGTGCCTGGATTGTGACAGTGCCACCTAAAAATGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCCGCAGCGTTGCCGGACGATCCCCACACACCTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGCGTCGGTTTTTTGGTTTGCCTTGTTATTCTCTAATTTTGTTCa                                       >  2:241043/1‑250 (MQ=255)                                                                                                                                                                                                                                ggATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAAGCCCGCTCa                      <  1:108768/250‑1 (MQ=255)                                                                                                                                                                                                                                            tgtCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAA‑GCCCGCTCATTAGGCGGGCTggg        >  1:495914/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAA‑GCCCGCTCATTAGGCGGGCTGGGTATCTg  >  1:150937/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        AACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGC‑TTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAA‑GCCCGCTCATTAGGCGGGCTGGGTATCTG  >  NC_000913/1322513‑1323004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑