Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,321,672	C→T	T425T (ACG→ACA)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,321,672	0	C	T	100.0%	185.5 / NA	62	T425T (ACG→ACA)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/31);  total (31/31)

CAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAACGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCG  >  NC_000913/1321426‑1321915                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCGCCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTcc                                                                                                                                                                                                                                                 <  1:344559/251‑1 (MQ=255)  aGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTccc                                                                                                                                                                                                                                                >  1:495162/1‑251 (MQ=255)           ggggttttGTTACGGGTTTCGTGGGATTCCGATTGCGGAACAGAATCAAGGTCTACACCAGCGCCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGGCGAGATCGCAAGGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGCCCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCAt                                                                                                                                                                                                                                       <  2:6214/246‑1 (MQ=255)               cTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATAc                                                                                                                                                                                                                                     >  1:564894/1‑249 (MQ=255)               cTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATAc                                                                                                                                                                                                                                     >  1:408105/1‑249 (MQ=255)                               gtcgGCTTCCGACTGCGAAACAGAATCAAGGACTACACCAGCACCCGCTTTCACGGTGGCGATAACGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGCAATAACCTAACGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTACCTGCATAGCGCGTCCTTTCGGCGCACAGCTTAATGTCCCCGTATCCCTACAGGcgcgtacagcgcgc                                                                                                                                                                                                                    >  2:165919/1‑240 (MQ=255)                                                      actcaaggacgtccgcAGCTCCCGCTGGCACGGTGGCGATACCGTCGACCACGCGCGCTGACCGGATCACTATGTGGGTGTCGATATCGCATTGCGCGGTGAAATAACCTACCTCGCCGCCGTAGCTGCCTCGGCGTCTACCGTCCGCCTCGGCAATTAACTGCATAGCTCGTACTTTCTGCTCACCGCGTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGGCAAGATCGTGActcggt                                                                                                                                                                                            <  2:84602/236‑6 (MQ=255)                                                         cAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCg                                                                                                                                                                                         >  2:244684/1‑251 (MQ=255)                                                           acggcgacgacatcatccGCGTGCACGCGGGCGATTCCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGGGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGTCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGcc                                                                                                                                                                                       <  1:552940/235‑1 (MQ=255)                                                             gACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCg                                                                                                                                                                                      >  1:325390/1‑250 (MQ=255)                                                               cgacacCAGCACCCGCTTGCACGGGGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACt                                                                                                                                                                                   <  2:441125/249‑1 (MQ=255)                                                                tACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCACCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGAACTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCAAAGCTTAATGTCCCCATAATCATACAGGCGCGAAAGGCGTGCAGGACGTCAAGATCGTGACGCAGTTCGCCGACt                                                                                                                                                                                   >  1:339694/1‑250 (MQ=255)                                                                     cAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTAcgcgg                                                                                                                                                                             >  1:568259/1‑250 (MQ=255)                                                                     cAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGCGAACTAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGCCCTTCCGCCTCGGCACTTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCCTCTTCCTACAGGCGCGGTACGCGTGCACGGCGCCAAGATCGTGACGCCGTTCGCCGCCTAcgcgc                                                                                                                                                                             >  2:221744/1‑250 (MQ=255)                                                                          cccGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGAc                                                                                                                                                                        >  1:35480/1‑251 (MQ=255)                                                                           ccGCTTGCACGGTGGCAGTACCGTTTCCCACCAGCGCCGAGCGGAGCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACg                                                                                                                                                                       <  1:281664/251‑1 (MQ=255)                                                                                 ggaCGGTGGGGATTCCGTTTTCAACAAGCGAGTAGCGGCTCACAATGCAGGGGGCGAGATCGGCATGCGCGGTGAAATAACCTTCCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTGCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCAGACTACGCGAGAGCCGAGGTg                                                                                                                                                                  <  2:489797/248‑1 (MQ=255)                                                                                 gcccGGTGGCGATCCCGTTTTCCACCAGCGCCGAGCGGATCACAATGCCGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGc                                                                                                                                                                 <  2:495162/248‑1 (MQ=255)                                                                                          cGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGAAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACAt                                                                                                                                                          >  1:374896/1‑249 (MQ=255)                                                                                                cGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGCTCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTCATGTCCCCATATTCATACAGGCGCGATAAGCGTGCACGGCGTCAAGATCGTGCCGCAGTTCGCCGACTACGCGCGAGCCGAGGTGCCTCACATAGGacaa                                                                                                                                                   >  2:91595/1‑247 (MQ=255)                                                                                                     tCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGt                                                                                                                                              <  1:244684/250‑1 (MQ=255)                                                                                                          caGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACtt                                                                                                                                        <  1:285258/251‑1 (MQ=255)                                                                                                           aGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATCGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCATGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATATGAATAACGGTCCACttt                                                                                                                                       >  2:506393/1‑251 (MQ=255)                                                                                                            tcgcCGAGCGGAACACAATGCAGGTGTCGAGATCGCCATGCGCGGTGACACAACCTACCCCGCCGCCGTAGCTGCAGCGGCGACGAACCTACGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCCCACCGCTCAATGTCCCCCTATTCATACCGCCGCGATAAGCGTGCAAGGCGTCAAGAGCGGGACGCAGTTCGCCGACTACGCGCGACACGAGGTGCATAACATAGTAAAAACGGTCAACTTTg                                                                                                                                      >  2:51815/2‑251 (MQ=255)                                                                                                                 gAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTg                                                                                                                                   >  1:209578/1‑249 (MQ=255)                                                                                                                             aTGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGt                                                                                                                      >  1:26585/1‑250 (MQ=255)                                                                                                                              ggCAGGTGTCGAGCTCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAg                                                                                                                    <  1:116623/250‑1 (MQ=255)                                                                                                                                  ggcgtCGAGATCGCCATGCGCGGTGAAATAACCTACAGAGCCGCCGTAGCTGACGCGGCGCCGACCTTCCGCCTCGGCAATTAACTGCCTAGCGCGTACTTTAGGCGCACAGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGGGCAGGGCGTCCAGATCGCGACGCAGTTCGCCGAATACGCGAGAGACGGGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATAGGCGTCGTAGCGGc                                                                                                                >  2:211609/4‑251 (MQ=255)                                                                                                                                  ggTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGc                                                                                                                >  1:567422/1‑251 (MQ=255)                                                                                                                                   gggccgagcgcgacATGTGCGGGGAAAAAACCTGCCGCGCCGCCGTAGCTGACGACGCGACGACCTTCCGCCGCGGCAATTAACTGCAAAGCGCGTACTTTCGGCGAACCGCTTAATGTCCCCATATTCATAAAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCTTCACATAGGAATAGCGGTCAATTTTGGTGAGATCGGCGACGTAGCGGcc                                                                                                               <  2:422956/238‑2 (MQ=255)                                                                                                                                    tgtggagaGCGCCATGCGCGGTGAAATAACCTACCGCGCAGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCt                                                                                                               <  2:499584/246‑1 (MQ=255)                                                                                                                                             cGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTg                                                                                                     <  1:8455/251‑1 (MQ=255)                                                                                                                                              gtcATGCGCGGTGAAATATCCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGTCAATTAACTGCATAGCGCGTACTTGCGGCGCACCGCGTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGGCAAGATCGTGACGCAGTTCGCCGACTACGCGAGATACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCTGGGGTGc                                                                                                    <  1:76927/249‑1 (MQ=255)                                                                                                                                                        gTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGGGCAGCACATAGAAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGGGCAAATGCgggc                                                                                          >  2:364626/1‑248 (MQ=255)                                                                                                                                                             aTAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGAt                                                                                     <  1:547598/251‑1 (MQ=255)                                                                                                                                                                         gccgccGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCgg                                                                         >  1:359346/1‑250 (MQ=255)                                                                                                                                                                                  gCTGCCGCGGCGACGAACTTCCGCCGCGGCAATTAACTGCATAGCGCGTACGTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCa                                                                <  2:352860/251‑1 (MQ=255)                                                                                                                                                                                    gtccgccgCGTCGACCTTCCGCCTCTGCTATTAACTGCATAGCGCTTTCTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGTGCTTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTTCCGGGGGTGCAAATGCGTGCCAGTTCATTACGGTCGAGATCAACCatc                                                              <  2:493422/249‑3 (MQ=255)                                                                                                                                                                                        gcggcgACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCa                                                          <  1:232313/251‑1 (MQ=255)                                                                                                                                                                                           gcgACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACtt                                                                                                                                        <  2:130652/170‑1 (MQ=255)                                                                                                                                                                                           gcgACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACtt                                                                                                                                        >  1:130652/1‑170 (MQ=255)                                                                                                                                                                                            ccaccacctcccccccccgCAATTTACTGCATAGCGCGTACTTTCGGCGCACCGCTTCATTACCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCTTCAAGATCGTGACGCAGTTCGCCGACTACGCGTGAGACGTGGTGCATCACATAGGAATAACGGTCATCTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCATCCAGCATCAGATg                                                      <  2:188740/233‑1 (MQ=255)                                                                                                                                                                                               cgcccTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGAGCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTc                                                   <  2:462106/248‑1 (MQ=255)                                                                                                                                                                                                   cTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGAc                                               >  1:270004/1‑251 (MQ=255)                                                                                                                                                                                                     tCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAATATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGTGGGTGCACATGCGTGCCAGCTCATTCCGGGCGAGATCAACCAGCATCAGATGTTccgcc                                               >  2:146289/1‑245 (MQ=255)                                                                                                                                                                                                     tCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGa                                                        <  1:297955/240‑1 (MQ=255)                                                                                                                                                                                                     tCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATAAACCAGCTTCAGaagagcggacg                                              >  2:297955/1‑240 (MQ=255)                                                                                                                                                                                                      cctccTCGGCAATTAACGTCATAGCTCGGACTGTCGGCGCCCCGCTTGATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTGCGGCGGCTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGAGCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATTATGACGGGCGAGATCAACCAGCATCAGATGTTCAGACAg                                             <  2:58563/247‑1 (MQ=255)                                                                                                                                                                                                        gCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGtt                                                    <  2:200154/241‑1 (MQ=255)                                                                                                                                                                                                        gCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGtt                                                    >  1:200154/1‑241 (MQ=255)                                                                                                                                                                                                             ggCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTAt                                     <  1:303441/251‑1 (MQ=255)                                                                                                                                                                                                               cAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATg                                    >  1:444894/1‑250 (MQ=255)                                                                                                                                                                                                               cAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGa                                   <  1:221744/251‑1 (MQ=255)                                                                                                                                                                                                                        gCATAGCGCGTACTGTCGGCGCACCGCTTAATGTCCCCATAGTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTAc                            <  2:261168/249‑1 (MQ=255)                                                                                                                                                                                                                              cgcgTACTTTCGGCGCACCGCTTAATGTCCCCAGATTCATACAGGCGCGATAAGCGTGCAGGGCGGCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCTGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTcc                    <  2:404122/251‑1 (MQ=255)                                                                                                                                                                                                                              cccgTACTTTCGGCGCCACGATTAATGGCCCCAGTTTCAGACAGGCGCGAGTAGCTGGCATGGCGTCAAGATCGTGACGGTGTTCGCCGTCTACGCGGGAGACGAGGTGCATCGCATAGGAATAACGGTCAACGTTGGTGAGGTCGGTGCCGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCAGCAGATGTTCAGACAGCGCTTTAGGATCGGTACGCAttgcc                    <  2:466146/249‑4 (MQ=255)                                                                                                                                                                                                                               gcgTACTTTCGGCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGCCGCAGTTCGCCGACTACGCGCGAGACGAGGTGCATCACATAGGAATAACGGTCACCTTTGGTGAGATc                                                                                                                              >  1:207129/1‑144 (MQ=255)                                                                                                                                                                                                                                 gTACTTTCGGCGCACCGCTTAATGTCCACATATTCATACAGGCGCGATAAGCGCGCAGGGCGACAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGAGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGACAGATCATTACGGGCGAGATCAACCAGCATCAGATGTGACGACAGCTCTTTATGATCGGTAAGCATGTCCAGt                 >  2:318685/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggCGCACCGCTTAATGTCCCCATATTCATACAGGCGCGATAAACGGGCAAGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATAATCGGTACGCATTTCCAGTTCAATACg         >  1:453348/1‑251 (MQ=255)                                                                                                                                                                                                                                              cccGCTTAATGGCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTGCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGt    <  2:563228/250‑1 (MQ=255)                                                                                                                                                                                                                                               ccGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTc   >  1:348055/1‑251 (MQ=255)                                                                                                                                                                                                                                                cGCTTAATGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCg  >  1:180136/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCCATGCGCGGTGAAATAACCTACCGCGCCGCCGTAGCTGCCGCGGCGACGACCTTCCGCCTCGGCAATTAACTGCATAGCGCGTACTTTCGGCGCACCGCTTAACGTCCCCATATTCATACAGGCGCGATAAGCGTGCAGGGCGTCAAGATCGTGACGCAGTTCGCCGACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCG  >  NC_000913/1321426‑1321915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑