Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	86.6 / NA	30	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/16);  total (14/16)

AGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCT  >  NC_000913/1306495‑1306978                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCtt                                                                                                                                                                                                                                           >  1:8345/1‑251 (MQ=255)                   tgaGGTCTCCCACAATCCACTACATCCTTACCCCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCg                                                                                                                                                                                                                          <  2:8345/250‑1 (MQ=255)                          tACCACAATCAACTACATCCTTACACCAGGGCATTGCTGTCGGCAGTCCCCATAACTGCTCCCGACCTGGAGAAGACCAAAACCATCCAGTTACTGCAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGATATGTTTTCCGTACCCGTTGCCCGATTGCAGGTCCAGACTGCGCCAAAACACGTCCTGTTGTGGTGGGGGGTTTCTGCCACGCCGTTTCTTACCTGCAAGTCGGTCCGCCTTaat                                                                                                                                                                                                                   >  2:90230/1‑249 (MQ=255)                                    cactaccaccTTACACAAGGGCATTGATGTCGTCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACCAAACCATCCAGATACTGGAAGGGGCATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGCTGCCCGATTGCCGGTCCCGCGTGCGCCAAAACACGTCCTGTTCTGGGGGGGGGTTTCAGACCCGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATaatgg                                                                                                                                                                                                        >  2:352901/9‑248 (MQ=255)                                     aCTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGGTTCTTGCCTGAAAGGCGATCCGCTTTAAAAACAATAAGGGc                                                                                                                                                                                                       <  1:149537/251‑1 (MQ=255)                                      atACAACCTTAAACCAGTGTATTGAAGTCGGCAATCCCCATACCGTAGCCGGAGTGGGAAAAGAACAAAACCAACCAGTGACTGGAAGGGGAAGTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGATTTCCGTACCCTTTGCCCGATTGCCTGACCAGAGTGCGCCAAAACACGTCTTGTTCTTGAGGGAAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCt                                                                                                                                                                                                      <  2:82349/250‑1 (MQ=255)                                        aCATCCTTACTCCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTGGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTTTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCt                                                                                                                                                                                                      <  1:352901/249‑1 (MQ=255)                                        aCATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTg                                                                                                                                                                                                     >  1:64895/1‑250 (MQ=255)                                          aTCCTTACACCAGGTCATTGATTGCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACa                                                                                                                                                                                                  <  1:336790/251‑1 (MQ=255)                                            ccTTACCCCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAACCCATCCAGTTCCTGGAAGGGGAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGt                                                                                                                                                                                                <  1:414477/251‑1 (MQ=255)                                               tcccccAGGGTATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCACCCAGTTACGGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGtt                                                                                                                                                                                               <  1:80933/245‑1 (MQ=255)                                                        gCATGGATGTCGGCAGGCCCCAGACCTGTTCCGGATTGGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATGCCCGTCGCCGATCAACCCGCCTGCCGGTTGTGTGTTCCGTACCCGTTGCCCGATTGCTGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCATACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAGAAACAATAATGGCTGACAGTTGTCAGCCCttt                                                                                                                                                                                    <  2:209808/251‑1 (MQ=255)                                                         cATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                                                                   <  1:133320/251‑1 (MQ=255)                                                         cATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCAATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                                                                   >  1:510116/1‑251 (MQ=255)                                                          aTTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTGACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAATGGCTGACAGTTGTCAGCCCttttt                                                                                                                                                                                  <  1:214828/251‑1 (MQ=255)                                                                                     tacggctcttgaacagaataacaCCATACAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCAGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCATGTTCTGGAGGGGAGTTTAAGACACGCCGTTTCTTGCCTGAAATTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTGCACGCAAAAAGCGATTCTTTATTcca                                                                                                                                                        >  2:378309/23‑249 (MQ=255)                                                                                                                         gtaaGGGGAATTAACGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGAACGACTGCCGGTCCAGAGTGCGCAAAAACACGTCCTGTTCTGGCGGGGAGTTTCAGACACGCAGTTTATTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTATCCGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCAGACAGATGCTATGGCAAAGTTTTTGATCTTTTTcgcg                                                                                                                   >  2:263217/3‑251 (MQ=255)                                                                                                                            aGGGTAATTCCCGTCCCCGATCAACCCGCCGTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGATTGCGGGTCCAGATTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTAGTCCCGCCAGATGATATGGCAAAGTATGTGATCTTTTTCGCGGCa                                                                                                                <  2:14062/251‑1 (MQ=255)                                                                                                                                gAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                                                            >  1:349065/1‑251 (MQ=255)                                                                                                                                     cccggcGCCGACCAACCCGCCTTCCGGTTGTGGTTTCCGTTCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTGTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa                                                                                                        <  1:378309/245‑1 (MQ=255)                                                                                                                                         tCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTACAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCgg                                                                                                   >  1:242145/1‑251 (MQ=255)                                                                                                                                                 cAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATATCAGAATGCGGGCaaaaa                                                                                            >  2:189877/1‑250 (MQ=255)                                                                                                                                                              ggTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGCGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAACGTTTGTGATCTTTTTCGCGTCATAACAGAATGCGGGCAAAAACATAGTTGATTTcc                                                                              >  2:253747/1‑250 (MQ=255)                                                                                                                                                                   tgtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATc                                                                         >  1:303321/1‑251 (MQ=255)                                                                                                                                                                              cccGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACAATCTTCACTGTCCg                                                              >  1:48230/1‑251 (MQ=255)                                                                                                                                                                                                    acatggctccAAAACACGTCCTGTTCTGGAGGGTAGTTTCAGACACGCCGTTTCTTGCCTTAAAGCCGATCCGCTTTAAAAACAATATGGGCTGCCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCTGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGc                                        <  2:316524/243‑1 (MQ=255)                                                                                                                                                                                                         gcgcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCaaaaa                                   <  1:498387/251‑1 (MQ=255)                                                                                                                                                                                                                                   gtgaattccAGACACGCCGGTTCTTGCCGGAAAATACATCCGCGTTAAAAACAATAAGTGCTTACAGTCGTCCGCCCTTTTTCACGCTAAAAGCGATTATGTATTCCCGCCAGATGATAGGGCACAGTTTGTGATCTTTTGCGCGGCATAACAGAATTCGAGCAAAAACATCGTTGCTTTCACCTTCTTCACTGGCCGCCAGACCAATCACCTCTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACa         <  2:115691/243‑1 (MQ=255)                                                                                                                                                                                                                                        tttCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAACAACACTAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAACAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGCATGCGGGCAAAAACATCGTTGTTTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGACAGGGGTAAAATCGAACTCAACATGTTc    >  2:40608/1‑251 (MQ=255)                                                                                                                                                                                                                                           caaacacGCCGTTTCTTGCCTGAAAGTCTATCCGCTTTAAAAACAATAAGGTCTGACCGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTGCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCt  <  2:349065/247‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   AGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCT  >  NC_000913/1306495‑1306978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑