Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,290,669 G→A W143* (TGG→TAG)  rssB → regulator of RpoS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,290,6690GA95.0% 53.3 / ‑3.5 20W143* (TGG→TAG) rssBregulator of RpoS
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (15/4);  total (16/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

ACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAC  >  NC_000913/1290427‑1290899
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      
cctcaTGAAAGGGCTTAAATGGCTTGATCATATACTGATCGGAGGCGACCGGACTCCAGTTCTGGTGATAGCTGACGCGGAAAATATGGCAGATATTGCCAAAGCGTGTCGTCTGGGCGTTGAATATGTGTTGCTGAATCCAGTTTAAGATATGAATCGCTTGCGCGAGATGGTTTTTTCCGGTCTCTATCCCAGCATGTTTAATGCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCaa                                                                                                                                                                                                                                <  2:189665/247‑1 (MQ=255)
           ggCTTAAACTGCTGGAGCATATACGTATCAGAGGCGACCAGACCCCAGTTCTGGTGATATCGGCCACGGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGGTTTTGCCTGCCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAAc                                                                                                                                                                                                                     <  2:381434/251‑1 (MQ=255)
                                       cAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGCAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAgg                                                                                                                                                                                         >  2:483584/1‑251 (MQ=255)
                                         gagGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGGAATTATTACAGGa                                                                                                                                                                                        >  2:67863/1‑250 (MQ=255)
                                         gagGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGa                                                                                                                                                                                        <  1:67863/250‑1 (MQ=255)
                                                ccAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAAc                                                                                                                                                                                >  1:123919/1‑251 (MQ=255)
                                                  aGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAAccg                                                                                                                                                                              >  1:302798/1‑251 (MQ=255)
                                                                                     atGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACAGCCGGTTCAGCAGGGGATTTCCCATTGCCGGGTTa                                                                                                                                            >  2:529947/1‑250 (MQ=255)
                                                                                                      aGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTg                                                                                                                          >  1:343106/1‑251 (MQ=255)
                                                                                                                cTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTAc                                                                                                                                                                                   <  1:193871/184‑1 (MQ=255)
                                                                                                                cTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTAc                                                                                                                                                                                   >  2:193871/1‑184 (MQ=255)
                                                                                                                      gTTGAAAATGATTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGTTGGTTTTTGCCTGTCTCTATCCAAGCATGTTTAATTCGCGCGTTGTGGAAGAGGACCGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAATTTATTACAGGAACTACAACCGCCGGTTCAGCAGGCGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCAGCGGACCAACCCgg                                                                                                          >  2:99872/1‑251 (MQ=255)
                                                                                                                                ttttGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCtt                                                                                                >  1:214715/1‑251 (MQ=255)
                                                                                                                                                     ttCTGAATCGCTTGCGCGTGATGGATTTTGCTTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGATGAGGAAAGGCTTTTTAGCGACTAGGATGCAAAGATTGATAACCATGCCACAGCGGCTAAATTATTACAGGAACAACAACCGCCGGTTCAGCAGGTAATTTCCCATTACCAGGTTAATTATCGTCACTTGTTTACCGAGGACAATCCTGGCCTGGGGATTGTTATTTCTGAACTTTCggt                                                                            >  2:97582/2‑249 (MQ=255)
                                                                                                                                                     aTCTGAATAGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCCTGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGTACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCATTAGGTTGCCGCGGCCAAACCCGACCTGGTGCTTGATATTGCAGCACTTTCggt                                                                            >  2:201460/1‑249 (MQ=255)
                                                                                                                                                                                        ctctATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCg                                        >  1:178717/1‑251 (MQ=255)
                                                                                                                                                                                                              cgcgcgTTGAGGAAGAGGAACGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCCCTTTCGGAAAACGATCTGGCCTTTTATTGCCTTGATGTCACCCGAGCTGGAAATAATGGc                        >  2:7780/1‑245 (MQ=255)
                                                                                                                                                                                                              cgcgcgTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACtt                  >  1:135618/1‑251 (MQ=255)
                                                                                                                                                                                                                 gcgTTGAGGAAGAGGAAAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgct               >  1:505415/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggaaAGGCTTTTTCGCGACTAGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAc  >  1:262310/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      
ACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAC  >  NC_000913/1290427‑1290899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: