Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,637	C→T	G201S (GGT→AGT)	ymfE ←	uncharacterized protein YmfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,637	0	C	T	100.0%	40.4 / NA	14	G201S (GGT→AGT)	ymfE	uncharacterized protein YmfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

CCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTG  >  NC_000913/1197411‑1197854                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          ccATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTAc                                                                                                                                                                                                   >  2:9559/1‑251 (MQ=255)        tctcAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACaaaaa                                                                                                                                                                                              >  1:305004/1‑249 (MQ=255)                     aaTATGCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCAGTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCa                                                                                                                                                                               <  2:73036/251‑1 (MQ=255)                                         tAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCACAGAACCTTCAACATGaaa                                                                                                                                                            >  1:518933/1‑250 (MQ=255)                                                                    cATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGatt                                                                                                                                <  1:367977/251‑1 (MQ=255)                                                                                       aaCTCCGTTCATATTATAGTTCAAATCAAGTGACATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATATTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTACAACAGTAAAAATATCCATTTGTTTTCAAAAAAAGATTATTAGGAAGGAAATTAATg                                                                                                             <  2:261615/251‑1 (MQ=255)                                                                                                cATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTGTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCg                                                                                                    <  2:133913/251‑1 (MQ=255)                                                                                                                            cAATTAATGTTCTTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGAATTTCAGTTCTGGGATTAACTGTCAAAGCATAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATAAATGCAATTATCGAAAATTAAAAAAATATCAAAAAATAGt                                                                         >  2:471952/1‑250 (MQ=255)                                                                                                                                       aTTTTTAGTGAAAAATTCTTTTATTTTAACGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                                             <  2:305004/251‑1 (MQ=255)                                                                                                                                         ttttAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                                             >  1:447293/1‑249 (MQ=255)                                                                                                                                             aGTGAAAAATTCTTTTATTTTCTCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTAGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                                          >  2:266110/1‑248 (MQ=255)                                                                                                                                                gAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGtt                                                    >  1:368228/1‑251 (MQ=255)                                                                                                                                                                  atCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCAAGAAGAGTTCAATATAATGTTTGTCtt                                  >  1:567573/1‑251 (MQ=255)                                                                                                                                                                                                  ttCTGGGCTTAACTGTCAAAGAAAAGATAATACTACTAACTGCGAATGATAAAATTACAAAAAAGAATAAAGCAAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTAGCACAAAAAGATTATTAGGAAGGTAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTAAATATAATGTTTGTATTCAATTTTTCTTACTTAAGGGTAATATAGCTTg  >  2:326278/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          CCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTG  >  NC_000913/1197411‑1197854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑