Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,191,644	C→T	E115K (GAA→AAA)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,191,644	0	C	T	97.6%	124.7 / NA	41	E115K (GAA→AAA)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/19);  minor base A (1/0);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTCAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGG  >  NC_000913/1191400‑1191882                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               gggTACTGGCGTTCCATACGGTAGGCGACGTTTGACATCTCTTTACCGATGGTTCACGGCGTGGCTGGCTGACCGTGTGTACGAGACAGCAGCGTGATATCGCGAAACTGAACGGCGAGATCTTTAATGCCATCAATCATTTGACGTCAGTATGGCAGGATCACTTCATCACGCGCGGTATTCAGCATTAATGCGTGGGAGAGGTTATTGATTTCTTCCGAATTACATGCAAAGTGGATGAATTTAGAAAc                                                                                                                                                                                                                                          <  2:501950/250‑1 (MQ=255)           gcTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTACTGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCCGCATTAATGCGGGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGt                                                                                                                                                                                                                                >  1:533259/1‑251 (MQ=255)           gcTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGt                                                                                                                                                                                                                                <  1:516895/251‑1 (MQ=255)                     gTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGAATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGGGGGGGAGGTTATTGATATCCTCCGAAGTAAAGGCCAAGTGGGTGAATTTAGAAACCGCGGGCAGTTCAGGGGtct                                                                                                                                                                                                                      >  2:423821/1‑251 (MQ=255)                        ggCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCg                                                                                                                                                                                                                   <  1:423821/251‑1 (MQ=255)                          cgccGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGAGTACGAGACAGCAGCGGGATATCGCGATACTGAAAAGCGAGATCTTTAATGCCCTAAATCAGTTGACGCCAGTCTGGCAGGATCAATTCAACAAGCGAGGTTTTCAGCCTTAATGCGTGGGTGCGGGTATTAATATCTTACGACGAACAGGCAAAGAGGGTGAATTTAGACACCACGGGCAGTTCCGGGGTCTCCGcc                                                                                                                                                                                                                 >  1:55948/4‑251 (MQ=255)                          cGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGGTATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGcc                                                                                                                                                                                                                 >  1:55949/1‑251 (MQ=255)                                 tGCCATCTCGTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACtttt                                                                                                                                                                                                           <  1:159022/250‑1 (MQ=255)                                    cATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAACAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGAGGGAACGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGCATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTtttctt                                                                                                                                                                                                       >  1:529761/1‑251 (MQ=255)                                          tttACCGATGGTTGACGGCGTGGCTGGCTGACCGTTGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCAGTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCa                                                                                                                                                                                                    <  1:572216/248‑1 (MQ=255)                                               cGATTGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGTTCTCCGCCACTTTTTCTTTCAGGAAATAc                                                                                                                                                                                            <  1:553423/251‑1 (MQ=255)                                                                       ggtcgggggTACGAGAAAGTAGCGGGATATCGCGATACGGAACGGCGAGAGCTTTAAAGCCATCAAGCAGGTGACGCCAGTATGGCAGGATCGTTTCATCACTCGCGGTTTTCACCATTAATGCGTGGGGGAGGTTATTGATATCTCCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTTCAGTACCGGGATCACCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGtt                                                                                                                                                                     <  2:439087/244‑1 (MQ=255)                                                                           gTGGGTACGAGACAGCCGCGGGCGAGCGCGATACTGAACGGCGAGATCTTTAATGCCCTCAATCAGTTGTAGCCAGTATGACAGGATCAATTCATCACGCGCGTGTTTCAGAATACATGCGTGGGAGTGGTTATTTATATCTTCCGAGGTACGGGCAAAGTGAGTGAATTTAGAAACCGAGTATAGTTCCGAGATCTCTGCCACATTTGTTTTCAGGACATACTCAACCTCTTTAAAGTCGTGGTCAGt                                                                                                                                                                  >  2:413598/1‑249 (MQ=255)                                                                           gTGGGAACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATAACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGGTATTGATTTCTTCCGAAGTACAGGCAAAGTGGATGCATTTAGAAACAGAGCGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCTGGAAATACTCAACCGCTTTAACGTCGTGGttttggg                                                                                                                                                                >  2:308944/1‑246 (MQ=255)                                                                             gggaaCGAGACAGCAGCGGGATATCGCGCTACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCATTTCATCACGCGCGGTTTTCAGCATTAATGTGTGGGAGAGGATATTGATATCTTCCGTAGTACAGGCAAAGTGGATGCATTTAGAAACCGCGTGCAGTTCCGGGATCTCGGCCACTTtttct                                                                                                                                                                                                        >  1:414640/5‑209 (MQ=255)                                                                               gTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGGGGGAGAGGTTATTGATATCTTCCGAAGGACAGGCAAAGTGGATGAATTTAGAAAACGCGTGCAGTTCCGGGATCTCCGCCAATTTTTCTTTCAGGAAATAATCAATCGCTTTAAcggtctgggtaaggggatg                                                                                                                                                            >  2:388511/1‑234 (MQ=255)                                                                                 aCGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGc                                                                                                                                                           <  1:308944/250‑1 (MQ=255)                                                                                   gagaCAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTCATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCg                                                                                                                                                        >  1:421519/1‑251 (MQ=255)                                                                                   gagaCAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCg                                                                                                                                                        >  1:137631/1‑251 (MQ=255)                                                                                                      gctagaCTGACCGGCGAGAGTTTTAATGCCATCAATCATTTGACGCTAGTATGGCAGGATCTCTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGTAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTGCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCGCGATAGTTTTGATGCGagccg                                                                                                                                     <  2:289888/246‑1 (MQ=255)                                                                                                                              aaTGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTCCAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGcg                                                                                                              >  2:171669/1‑250 (MQ=255)                                                                                                                                               gACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTa                                                                                             >  1:410849/1‑250 (MQ=255)                                                                                                                                                aCGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAAc                                                                                           >  1:482250/1‑251 (MQ=255)                                                                                                                                                  gCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTAAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCg                                                                                         >  1:221663/1‑251 (MQ=255)                                                                                                                                                        aTGGCAGGATCAGTTCATCACGCGCGGTTTTCATCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGGGGATGAATTTAGAAACCGCGGGCAGTTCGGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGc                                                                                    <  1:556162/250‑1 (MQ=255)                                                                                                                                                                 tCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGgcagc                                                                           >  1:187108/1‑250 (MQ=255)                                                                                                                                                                   aCTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCaaa                                                                        <  1:115830/251‑1 (MQ=255)                                                                                                                                                                                 ggTTTTCAGCATAAATGCGTGTGAGAGGTTTTTTATATCTTCCGAAGTACAGTCAAAGTGGAGGAATTTAGAAACCGCGTGCAGTTCCGGGAACGCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTGCTTCGCTGAAACGGGCGACGATTGCATCAAGGTAACCGAGTGCGGCGGCAGCAAAAGCAGGAACTTCCt                                                          <  2:533259/251‑1 (MQ=255)                                                                                                                                                                                       cAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTTGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCg                                                    <  1:74957/251‑1 (MQ=255)                                                                                                                                                                                           aTTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCtgc                                                <  1:169665/251‑1 (MQ=255)                                                                                                                                                                                                         gagGTTATTGATATCTTCCGAAGAACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGGGCGCGGCCAGtt                                  >  2:264334/1‑251 (MQ=255)                                                                                                                                                                                                          agGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCAGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTATTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGACGACGAATGCATCAAGGTAACCGCTTGCGTCGGCAGCAAAAGCAGGAACTTCCTTTATCGCTGCGTGCGCGGCCAGttt                                 >  2:335251/1‑251 (MQ=255)                                                                                                                                                                                                                         tCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGCAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTAc                  >  1:230322/1‑251 (MQ=255)                                                                                                                                                                                                                         cccgagatcatggCAAAGTGGATGAATTTAGAAACCGCCGGCAGTTCCGGGAACTCTGCCACTTTTTCGTTCAGGAAATACTCAACCGCTTTAACGTCGTTGTTAGTGGTACGCTCGACAGTTTTGATGCGCGCCGCAGCTTCTTCGCTTAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGAGCGCTGCGTGCGCGGCCAGTTTTTGCAGTCAACGTAc                  <  2:474598/240‑1 (MQ=255)                                                                                                                                                                                                                           cGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACtt                <  1:77273/251‑1 (MQ=255)                                                                                                                                                                                                                                  taGGCAAAGTGGATGAATTTAGAAACCGCGAGCAGTTCCGGGATATCCGCCACTTTTTCTTTAAGGAAATACTCAACCGCTTTCACGTCGTGGTTAGTGGTACGATCGATAGTTTTGATGCGAGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCACGGTAACCGATTGCGTCGGCAGCAACAGACGGAACTTCCTTGATAGCTGAGTGCGCGGCCCGTTTTTGCAGCCAACGGACTTCAACt           >  2:356076/2‑249 (MQ=255)                                                                                                                                                                                                                                  cAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTg         <  1:172805/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGTCGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTac      <  1:12560/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAAAGTGGATGAATTTAGAAGCCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTaca     <  1:505536/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCCGCCAACGAACTTCAACTTGTACACg   >  2:425424/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACgg  >  1:340505/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               CGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTCAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGG  >  NC_000913/1191400‑1191882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑