Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,015,891	C→T	intergenic (+9/+61)	rmf → / ← fabA	ribosome modulation factor/beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,015,891	0	C	T	100.0%	88.6 / NA	27	intergenic (+9/+61)	rmf/fabA	ribosome modulation factor/beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/16);  total (11/16)

AACTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACC  >  NC_000913/1015646‑1016125                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           aaCTGTGATTGCACATTTAGGAATCACTGTTTTATTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGAGCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATAGCCTGATTCTGTCTTTTTa                                                                                                                                                                                                                                        <  2:349924/250‑1 (MQ=255)            cacaTTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTc                                                                                                                                                                                                                            <  2:426072/251‑1 (MQ=255)                 ttAGTAATCACGGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCGATCAGACGCGGAATCAAAGGTCACAATGGCTGGTAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCa                                                                                                                                                                                                                        <  1:532794/250‑1 (MQ=255)                    gTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCTCATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGc                                                                                                                                                                                                                    <  1:411228/251‑1 (MQ=255)                                       ccaccaGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCGCATCAACGTGGTTATCAGGCCGTCATCGCCGGACGCTCAAAAGAAATGTGGCCCTATCAGACGCTGAATCAAAGGTCACAATGGCGGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGTCCTGATTCTGTCGTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTGTTGAt                                                                                                                                                                                                 <  2:543293/251‑1 (MQ=255)                                           caGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCGCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCGCCGCATTGCGGAGGTTTCGCCTTTTGATActc                                                                                                                                                                                             <  2:281295/251‑1 (MQ=255)                                                                            agaCAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCt                                                                                                                                                            <  1:266025/251‑1 (MQ=255)                                                                               cAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTAACAATGGCTGGGAGGCTGGCGAAAAACCATGGTGGACCGGGTAGTAATGGCATGATTCAGTCGTTTTAAAAAGCAACCTCCGCATTGACGCGGTTTCGCCTTTTGCTTCTCTGACTGCTCATAATCCGAAGGAAGaaggatacggg                                                                                                                                                          >  2:392531/1‑240 (MQ=255)                                                                                  aaaCGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCAAGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACa                                                                                                                                                      <  1:527025/251‑1 (MQ=255)                                                                                            gCCTGGAACGGGCACCTCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGATAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCCGAAGGCAGACGTACCCTAGAACAGACCGCCttt                                                                                                                                            >  2:167571/1‑251 (MQ=255)                                                                                                   aCGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCg                                                                                                                                     >  1:477464/1‑251 (MQ=255)                                                                                                      ggCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTg                                                                                                                                  >  1:214134/1‑251 (MQ=255)                                                                                                                                  aTCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCa                                                                                                      <  1:474788/251‑1 (MQ=255)                                                                                                                                       cGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACt                                                                                                 <  2:323673/251‑1 (MQ=255)                                                                                                                                         gACGCTCAAAAGCAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGCAGCCATGGCGGACAGGGCCGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATCATCAGTAGGCAACCGTATCCTGGCACAGACCGTCTTTCAGGTCGCTGGCGGTATAGCTCAGTCGGCCATCaaacaccccttc                                                                                               >  2:24123/1‑241 (MQ=255)                                                                                                                                           cGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCa                                                                                                                                                                                                                        <  2:10584/128‑1 (MQ=255)                                                                                                                                           cGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCa                                                                                                                                                                                                                        >  1:10584/1‑128 (MQ=255)                                                                                                                                                   aGAAATGTGGCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGcc                                                                                     <  1:167571/251‑1 (MQ=255)                                                                                                                                                    gAAATGTGTCCCTATCAGACGCTTAATCAAAGGGCACAATGGCTGGGAGGATGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTACCCTGGAACAGACCGACTGTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGcta                                                                                    <  2:90884/251‑3 (MQ=255)                                                                                                                                                      aaTGTGTACCTATCAGACGCTGAATCAAAGGTCACAATGACTGGGAGGCTGGCGAGAAGCCCTGGCGGACAGGGTAGTACTAGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGAGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTCTAGATCATACACCCAACAACCAGCAcatacccctacgcccg                                                                                   >  2:376347/1‑235 (MQ=255)                                                                                                                                                       aTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGc                                                                                 >  1:472848/1‑251 (MQ=255)                                                                                                                                                                   cAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTAGAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac                                                                     <  1:68403/251‑1 (MQ=255)                                                                                                                                                                            aaTCAAAGGTCACAATGGCTGGGAGTCTGGCTAGAAGCCATGGCGGTCAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTTATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTGCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTaa                                                            <  2:561379/251‑1 (MQ=255)                                                                                                                                                                                             gCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGa                                           <  1:181008/251‑1 (MQ=255)                                                                                                                                                                                                                             gggTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTCTAGATCAGACGAACATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGGGAAGACGATAGGCCACTTTTTTCGCTGTCggaa            >  2:136879/1‑248 (MQ=255)                                                                                                                                                                                                                                  gtaATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTATGTATGATTATAATCAGCAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCCTCAACCAGCACTTCGCCCTCCGCAAGGCACATAATCAGACGCCGGTTAAACATGCGTTTAAAGCGAATCAGGTAGGTCACTTTTTTctctctgcgacgttcct      >  2:248551/1‑235 (MQ=255)                                                                                                                                                                                                                                        gCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAcc  >  1:371952/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           AACTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACC  >  NC_000913/1015646‑1016125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑