Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,108	G→A	intergenic (‑177/‑295)	caiT ← / → fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,108	0	G	A	100.0%	90.5 / NA	29	intergenic (‑177/‑295)	caiT/fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

ATGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGGCGTGATA  >  NC_000913/41861‑42345                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aTGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGGTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTTTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGc                                                                                                                                                                                                                                            <  2:471237/251‑1 (MQ=255)          attattAACGGAGGAAAAAAAACCTTTGGTTCTATTCCCGTTTTTCTATGTTCATTCTTCATGAGGTAATTCCTCTGTTAAAACGAATATTTATTTTGCGTTCCCGTATGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGAAGCGTTAATGGCTAACTCATAATGGGTATACAATAATCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGtttttttg                                                                                                                                                                                                                                   <  2:424127/251‑3 (MQ=255)             attaACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTGTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGtt                                                                                                                                                                                                                                 <  1:529507/250‑1 (MQ=255)                        aaagaaaCCCTTCGGTTCTATTCACGTTTTTCTCTTTTCCATCTTCATGCGATAATTCCACTGTGAAACCGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTAGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATACGCTGTATTCTTTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATta                                                                                                                                                                                                                      >  2:326222/1‑250 (MQ=255)                        aaagaaaACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATtaa                                                                                                                                                                                                                     <  1:48236/251‑1 (MQ=255)                             aaaCCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGTTTAATAATc                                                                                                                                                                                                                >  1:36032/1‑251 (MQ=255)                                              cccGTTTTTCTCTTTTCCTTCTTCATGAGTTAATTCCACTGTGCAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGTTTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGTTTAATAATCAGTTTGTTCTGCTCtgt                                                                                                                                                                                               >  2:210187/1‑251 (MQ=255)                                                              cATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATa                                                                                                                                                                                >  1:426497/1‑250 (MQ=255)                                                                                      gtgAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTg                                                                                                                                                         <  1:566690/249‑1 (MQ=255)                                                                                                    ttatttTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAata                                                                                                                                          <  1:489825/250‑1 (MQ=255)                                                                                                                 ccGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCttt                                                                                                                            >  1:159647/1‑251 (MQ=255)                                                                                                                  cGTTTTTTTTATTTTTGTTAACTTTTAATATAAGTATTATTAAACTAGTGTAAGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTTGTATCACATTTTTGTTTCGGGTTAATAGAAGTCGTTTTTTCGTTAATTTTGATTAATAATCATTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGTCTTTCAATATTTGGGATCCATTAAACAATATTGAAAATTTCtttt                                                                                                                           <  2:334334/251‑1 (MQ=255)                                                                                                                        ttttAGTTTTGTTAACAATTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAAATCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTTAATATAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGCTCCATAAAGCAATATTGAAAAGTTCttggtgcta                                                                                                                      <  2:39909/250‑8 (MQ=255)                                                                                                                              ttttGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCgtg                                                                                                               >  1:462084/1‑251 (MQ=255)                                                                                                                                gtgtgatcaattAATATAATTAATATTAACCTCGTGTACGTGGTAATTGCTAGCGCATAATGGTTACTCAATAAGCATTATTATGTGATTTGTATCACTGATTAGTTTCTGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGTTGAATAATCAGTGTATTATGCTCTGTTGTGAGTAAAAAATAACATCTGCCTTTCAATATTGGTGATCCTTAAAACAATAGTGAGAATTTATTATTGCTACGCCgtgg                                                                                                              <  2:143096/240‑2 (MQ=255)                                                                                                                                                      ttattaACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATtaa                                                                                                                                                                                                                     <  1:254711/125‑1 (MQ=255)                                                                                                                                                         ttaACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAAc                                                                                     >  1:245353/1‑250 (MQ=255)                                                                                                                                                             ccTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGCCTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAatat                                                                                >  1:528738/1‑251 (MQ=255)                                                                                                                                                                 gttgACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTTGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATAGTGaa                                                                            <  2:148475/248‑1 (MQ=255)                                                                                                                                                                                           aTGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACAtt                                                  >  1:266391/1‑251 (MQ=255)                                                                                                                                                                                             gggTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAATGTTGGATTTATCTGCGTGTGACAtttt                                                >  2:407402/1‑251 (MQ=255)                                                                                                                                                                                                 aTTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACCTTTTCAat                                            >  2:111531/1‑251 (MQ=255)                                                                                                                                                                                                      aTAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTgg                                       >  1:107244/1‑251 (MQ=255)                                                                                                                                                                                                      aTAAGCTGTAGCCTGCGATAGGTATCACATTTTTGCTCCGGGTGAATAGAAAGCGTTTTTTTGTTCATTTTGATTAATAATCAGTTTGTTCTGCTCTGTTGTGAGTCAAAAATAACATCTGCCTTTCAATATTGGTGATCCATAATACACTATTGCAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGCGGAGCTTAACAATATTGAAAGTTGGGTTTATCTGCGTGTCACATTTTCATTATTgt                                       >  2:235105/1‑250 (MQ=255)                                                                                                                                                                                                        aaGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTg                                     <  1:278876/251‑1 (MQ=255)                                                                                                                                                                                                           cTGTATTCTGTACTTTGTATCACATTTTTGTTTCGGTTGAATCGAAGGCGTTTTTTCGGTAATTTTGTTTAATAATCAGTTTGTTATGTTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCCATATTGGTGAGGAACTTAACAATATTGATAGCTGGATTTATCTGGTTGTGACTTTTTCACTATTGGTGAtt                                  >  2:196529/1‑251 (MQ=255)                                                                                                                                                                                                             ttATTCTTTTATTGATATCACATTTGTGTTTCGTGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGTCTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTGCAATATTGGTGAGGAACTTAACAATATTGAAAGGTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGa                                    <  2:29569/246‑1 (MQ=255)                                                                                                                                                                                                                            tATCACATTTTTGTTTCGGGTTAATAGAAGGAGTTTTTTCGTTAATTTTGTTTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTTAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGATGAACTTAACTATATTGAAAGTTGGATTTTTCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCaaa                  >  2:240152/1‑250 (MQ=255)                                                                                                                                                                                                                                            cGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGGCGTGata  <  1:285688/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              ATGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGGCGTGATA  >  NC_000913/41861‑42345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑