Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	97.2 / NA	30	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/18);  total (12/18)

GGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696232‑696705                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              ggCGTGTTGAAACTTCAGGCGCTGAAATACGCGTTAGGGTTGTAGCCTTTCAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                                                                                                                     <  2:311170/249‑1 (MQ=255)  gCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGcc                                                                                                                                                                                                                                  <  1:120983/251‑1 (MQ=255)         gAAACGTCAGGGGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGAGGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                                                                                                                            <  2:10147/250‑1 (MQ=255)              gTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                                                                                                                      >  1:27646/1‑251 (MQ=255)                               gCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGGCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                                                                                                                      <  2:128666/250‑1 (MQ=255)                                   tAGGGTTGTAGCCTTACAACAGTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGGGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCGTGGCGATACCCCAACTGGGTGCAc                                                                                                                                                                                                  <  2:35775/250‑1 (MQ=255)                                                         ggCCGGAGGCGTGCCAACCGTAGGTCGGATAAGCCGCGGCTGCGTCGCATCCGACATTGAAGGATAAGACGGGTCACCATCGCATTCGTCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAAt                                                                                                                                                                          <  2:166381/249‑1 (MQ=38)                                                             gtaTGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCACATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTTGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAAtt                                                                                                                                                                     <  2:467490/249‑1 (MQ=255)                                                              gATGCGTGCCACCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGCCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCTC‑TTACAAGGTAA‑GCGTCTTGAATAAATTg                                                                                                                                                                    <  1:169803/251‑1 (MQ=255)                                                                   gTGCCAACCGGAGGTCAGGTAAGACGCGTCAGCGTCGCATCCGACATTGTAGGATAAGACGTGTCAACATCGCATGCGACATTTAATGAACGCAGAAAAGCCATAAGCTCGCCGCAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGTACCTCGGAATGCCGGAATTATAATCCGGTGCCTTACCGCTTGGCGATACCCAAACTTGGTGCAC‑TTACAATGTAA‑GCGTCTTGAATAAATTGGCTgt                                                                                                                                                               <  2:316626/251‑2 (MQ=255)                                                                    tGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGGGGTAACCCAACTGGGTGCAC‑TTA‑AAGGTAACGCGTATTAAATCAATTGTCTggg                                                                                                                                                              >  2:304158/1‑251 (MQ=38)                                                                      ccatcagTAGGGCGGAGAAGACGCGGCCGCCTTGCATCAGATAGTGAAGGATAAGACGTGATAACATCGTATGCGACATTGAATGAACGCAGAAAAGCAAAAAGCGCGCCGAATCGAGATTTTTTATTGTGGCTGGGGCACGAGGATTCGACCCTCGGAATGCCGGAATTAGAATCCGGTGTCTTACCGCTTGGCGATACCCCAAATGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGt                                                                                                                                                            <  1:24784/245‑1 (MQ=255)                                                                         aCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACg                                                                                                                                                         <  1:469129/251‑1 (MQ=255)                                                                                             cgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCC‑TTAAAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTTCGCGGATTCGAACCTCGGAATg                                                                                                                                     >  1:572008/1‑251 (MQ=255)                                                                                                  gCGTCGCATCCGACAGTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                                                                                                                                <  1:173296/251‑1 (MQ=255)                                                                                                    gTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑ACGTCTTGAATAAATTGGCTGGGGTACGAGGCATCGCACCTCGGAATGCCGGaa                                                                                                                               >  1:205954/1‑250 (MQ=255)                                                                                                                                 gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                 <  1:221959/251‑1 (MQ=255)                                                                                                                                                     tgaatgtattcAGAAAAGCAACAAGCTCGCCGAATCGAGCTTTGTTAATGAGGCTGGGGTACGAGGATTCGATCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGACTGCCGGAATCAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACAAATTGGtttt                                                                              <  1:451024/240‑1 (MQ=38)                                                                                                                                                                 gAAAAGCAAAAAGCTCGCCTAAGCGAGCTTATTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc                                                                 <  1:341011/251‑1 (MQ=255)                                                                                                                                                                         aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGTCTGGGGTACGAGGATTCGAACCTCGGATTGCCGGAATCAGAATCCGGTGCCTTACCGGTTGGCGATACCCCAACCGATTGGGTTTGAGTTGGCCgtccatcttcg                                                          >  1:197275/1‑240 (MQ=37)                                                                                                                                                                            aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGAG‑ACGTTACAAGGTAA‑GCGACTTGAATAAATTGTCTGGGGTACGAGGATTCGAACCTAGGGATGCCGGAATCAGAATCCGGTGCCTTACAGAGTGCTGATACACCCACAAATTGGTTTTGAAtgtaactaacaaattcgaagc                                                      >  1:566030/1‑231 (MQ=21)                                                                                                                                                                                cGCCGAGGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAC‑GCGTCTTGACTAAATTGGCTGGGGCACGCGGAGTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGCATATTCCGCACatatgaggaacattc                                                  >  1:281621/1‑240 (MQ=37)                                                                                                                                                                                   cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                                                                                                                               >  1:468153/1‑155 (MQ=255)                                                                                                                                                                                   cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                                                                                                                               <  2:468153/155‑1 (MQ=255)                                                                                                                                                                                             ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGATTTtggtggg                                     >  1:72422/1‑250 (MQ=255)                                                                                                                                                                                              tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGATTTTGGTGGCt                                    >  1:109262/1‑251 (MQ=255)                                                                                                                                                                                                  tAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGGCA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGGATGCCGGCATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTAGATACATTCAGAATTtggtggacacg                                 >  1:239870/1‑245 (MQ=38)                                                                                                                                                                                                        ggCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCTAACATATGCGATACATTCAGAATTTGGTGGCTACGACGGGAt                          <  1:443289/251‑1 (MQ=255)                                                                                                                                                                                                                   gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGAAGGGATTAGGACCtgtg               >  1:435130/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAt  <  1:303519/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAC‑TTACAAGGTAA‑GCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696232‑696705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑