Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,683	C→T	N289N (AAC→AAT)	carB →	carbamoyl‑phosphate synthetase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,683	0	C	T	100.0%	164.2 / NA	57	N289N (AAC→AAT)	carB	carbamoyl‑phosphate synthetase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/34);  total (23/34)

GTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGAC‑TATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCG  >  NC_000913/31440‑31918                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                gTCGCTGATCGGCTGGAAAGAGTACTAGATGGAAGTGGTGCGTTATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACCCCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGaaaa                                                                                                                                                                                                                                          <  1:3091/251‑1 (MQ=255)       gATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGgt                                                                                                                                                                                                                                     <  1:129703/250‑1 (MQ=255)       gATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAAGCATGCGTAACGTCTCGATGGC‑GGTGCTGCGTGAAAGCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACgg                                                                                                                                                                                                                                      <  1:545717/249‑1 (MQ=255)       gATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCGCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AATCCGGTGGTTCCATCGTTCAGTTTGCGGTGAATCCGAAAAACGgtc                                                                                                                                                                                                                                    <  1:323663/251‑1 (MQ=255)           ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCt                                                                                                                                                                                                                                >  1:110316/1‑251 (MQ=255)           ggCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTAGCGCCAGCCCAAACGCTGCCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCATGAAATCGGCGTTG‑CAACCGGTGGGTCCAACGTTCAGTTTGGGGGGAATCCGGAAAAAGGTCGTCt                                                                                                                                                                                                                                >  2:30851/1‑251 (MQ=255)              tgaaaaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAATTCGGCGTTG‑AAACCGGGGGTTCCAACGTTAAGTTTGCGGTGAATCCGAAAAACGGGCGTCTggg                                                                                                                                                                                                                             >  2:506307/4‑249 (MQ=255)               ggAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAACCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGCTGCGATGAGCAACCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAATCATATCCAATCATGCGTAACGACTCGATGGC‑GGTGCTGCGTGAAATCGGCGTAC‑ACACCGATGGTTCACACGTTCAGTTTGCGGGGAATCCGACAAACGGCCGTCTgctt                                                                                                                                                                                                                            >  2:428197/1‑248 (MQ=255)                      gTACGAGAGGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCTATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCg                                                                                                                                                                                                                     <  2:287973/251‑1 (MQ=255)                          gagaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAAt                                                                                                                                                                                                                 <  1:338651/251‑1 (MQ=255)                                   gtggtgCTGGATAAAAACGACAACTGCACCATCGTCTGCTCTATCGAAACCTACGATGCGATGGGCATCCACACCGGTGACTCCATCTCTGACGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACCCCTCGATGGC‑GGGGCTGTGTGAAATCCGCGTTG‑AAACCGGTGGTTCCAACGTCTAGTTTGCGGTGAATCCGAAAAACGATCGTCTGATTGTTATCGAAATGAACCCAcg                                                                                                                                                                                                        <  2:207805/251‑1 (MQ=255)                                   gtggtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAACCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGAGTGAAATCGGCGTTG‑AAACCGGTGGTTCCCCCGTTAAGTTTTCGGGGACTCAGACAAACGGTCGTCTGTGTGTTATCGCAATGCACCCAcc                                                                                                                                                                                                        >  2:227529/1‑250 (MQ=255)                                      gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACTCCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCgt                                                                                                                                                                                                     <  2:477611/251‑1 (MQ=255)                                      gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGGTTGTTATCGAAATGAACCCACGCgt                                                                                                                                                                                                     >  2:96359/1‑251 (MQ=255)                                            gATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGATCCCACGCGTGTccc                                                                                                                                                                                                <  1:428197/250‑1 (MQ=255)                                            gATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCg                                                                                                                                                                                               <  1:510242/251‑1 (MQ=255)                                             aTAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCAATGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGAGGTG‑AAACAGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACACACGCGTGTCCCGt                                                                                                                                                                                              >  2:431472/1‑251 (MQ=255)                                                      aCAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTcc                                                                                                                                                                                                 >  2:175293/1‑239 (MQ=255)                                                      aCAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTcc                                                                                                                                                                                                 <  1:175293/239‑1 (MQ=255)                                                        aaCTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAACTGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                                                                                   >  1:456106/1‑251 (MQ=255)                                                           ttcatcaaattcatctctATCAAAAACTTCGATGCGATGGGCATCCACACCGGTAACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGATCCAACGGTCAGTTTGCGGTGAATCCGCAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGACGCTG‑Gc                                                                                                                                                                               >  1:308025/15‑251 (MQ=255)                                                             catcatCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGt                                                                                                                                                                             <  1:431472/251‑1 (MQ=255)                                                             catcatCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCGCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGGCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGt                                                                                                                                                                             <  2:193677/251‑1 (MQ=255)                                                                   cGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAg                                                                                                                                                                       <  2:48975/251‑1 (MQ=255)                                                                         ctctATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACAGGAGGCTCCAACGTTCAGTTTGCGGGGAATCCGCAAAACGGTCGTCTGATTGTTATCGAAATGACCCCACGAGCGTCCCGTTCTTCGGGACTGTGC‑TCGAAAGCGAc                                                                                                                                                                   >  2:228209/1‑249 (MQ=255)                                                                            tATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGtt                                                                                                                                                              >  1:309216/1‑251 (MQ=255)                                                                                 aaaaCTTCGATGCGATGGGCATCCCCACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAGCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCg                                                                                                                                                         <  2:476971/251‑1 (MQ=255)                                                                                     cTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGCATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGATTCCCGATTg                                                                                                                                                     >  1:344628/1‑251 (MQ=255)                                                                                       tCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTg                                                                                                                                                     <  1:245897/249‑1 (MQ=255)                                                                                        cgatgcgatgGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATAATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAAAATTCAGTTTGCGGAGAATCCGAAAAAAGGTCGTCTGATTGTTGTCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTT‑GGTTCGAACGCGACCGGTTTccacatttct                                                                                                                                                   >  2:465324/1‑242 (MQ=255)                                                                                        cgatgcgaggGGCATCCACACCGGTGACGTCATCACTGTCGCGCAAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGAGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGGTATCGAAATGAACCCACGCGTGTCCCGTTCTACGGCGCTG‑GCGTCGAAAGCGACCGGTGTCCCGATTGCTa                                                                                                                                                  <  2:277786/251‑1 (MQ=255)                                                                                             cgatgGGCATCCACACCTGGGTCTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                                                                                             <  2:9873/251‑1 (MQ=255)                                                                                                ggggCATCCACACCGGTGACTCCAGCACTGTCGCGCCAGCCCAAACGCTGACCGACAAATAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGTCGTTG‑AAACCGGTTGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCCGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGGCCAAAGCGACCGGGTTCCCGATTGCGAAAGTggcg                                                                                                                                          <  2:366524/250‑1 (MQ=255)                                                                                                       ccACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                                                                                    <  1:96359/250‑1 (MQ=255)                                                                                                           cccGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGc                                                                                                                               <  2:237338/250‑1 (MQ=255)                                                                                                                      cATCACTGGCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑aca                                                                                                                   <  1:550684/251‑1 (MQ=255)                                                                                                                      cATCACGGTCGCGTCAGCCCAAACGCTGACCGAGCAAGAATAGCAAATCAGGCGTAATGGCTGGAGGGC‑GGTGCGGAGTGAAATCGGCGTTG‑TATCCGGGGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGGCGGCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGGTCTTCGGCGCGG‑GCGTCGAAAGCGACCGGCTTCTCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGTT‑aca                                                                                                                   <  2:227113/251‑1 (MQ=255)                                                                                                                             gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGT‑ACAcccccgg                                                                                                            >  1:235727/1‑247 (MQ=255)                                                                                                                               cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑TCAccccccacc                                                                                                          >  1:117195/1‑245 (MQ=255)                                                                                                                                        ccAAAGGCTGACAGACAAAGACTCTCAAAGTATGCGTGCCGGCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCTAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGGCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGTCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACtgatga                                                                                                 <  2:100533/251‑1 (MQ=255)                                                                                                                                           aaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGT‑ACAccccccacgcaacgttgaa                                                                                                >  1:259267/1‑233 (MQ=255)                                                                                                                                           aaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAAAGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGGGGCGGCGAAACTGGGGGGGGGGT‑TCACCCCcgacaaaacaatagac                                                                                               >  2:440091/1‑238 (MQ=255)                                                                                                                                               cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTcgac                                                                                                           <  1:248217/234‑1 (MQ=255)                                                                                                                                                         aaGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGCACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTTAC‑CCCCCGACGAACTGATGAACGACaatcctagcggg                                                                                >  1:87242/1‑240 (MQ=255)                                                                                                                                                          aGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGT‑TCACCCCCGACGGAATGATGAAAGACATCCCTGGCgg                                                                                 >  1:102011/1‑249 (MQ=255)                                                                                                                                                                  aaattagtcGTACCGCCTCTATGGCTGGT‑CTGCGTTAAATCGGC‑TGGTAAACCGGTGGTTCCCACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTTATTTTTATCGAAATGACCCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCTACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑CCACCCTCGACGAACTGATGAACGTCATCACTGGCGGACTTTCT‑CCg                                                                      <  2:76113/243‑1 (MQ=255)                                                                                                                                                                                gCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGcc                                                        <  1:144743/251‑1 (MQ=255)                                                                                                                                                                                     gATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACt                                                                                      <  1:227582/217‑1 (MQ=255)                                                                                                                                                                                        ggC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATc                                                 <  1:563264/250‑1 (MQ=255)                                                                                                                                                                                               gctgcGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGAC‑TAtg                                         <  1:150770/251‑1 (MQ=255)                                                                                                                                                                                                ctgcGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                                                                         <  1:212291/110‑1 (MQ=255)                                                                                                                                                                                                ctgcGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                                                                         >  2:212291/1‑110 (MQ=255)                                                                                                                                                                                                      gAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGAC‑TATGTGGTTa                                   <  1:35605/250‑1 (MQ=255)                                                                                                                                                                                                           cGGCGTTG‑AAACCGGTGGTGCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTGCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGAC‑TATGTGGTTACTaaa                              <  1:206039/250‑1 (MQ=255)                                                                                                                                                                                                                        cGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGT‑ACACCCCCGACGGACCGATGGACGAAAACACTTGCGGGCGGAAT‑CCCGCCCCCTTCCAGCCCTCCCTCGACCTATGGGGTAATTAAAATTcccccctt                   >  1:496313/1‑244 (MQ=255)                                                                                                                                                                                                                                    cGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGT‑ACACCCCCGACGAACAGATGGACGACATCACTGGGGGGGGTACTACCGCCCCCCTT‑GAGGCGGCCATCCAC‑ACTGTGGGTACTAAAAATTCTCGCGTCAACTTTGaaaaa      >  1:442072/1‑250 (MQ=255)                                                                                                                                                                                                                                        cAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCGG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGAC‑TATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCg  <  1:386745/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                GTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGC‑GGTGCTGCGTGAAATCGGCGTTG‑AAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTG‑GCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTT‑ACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGAC‑TATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCG  >  NC_000913/31440‑31918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑