Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,517	C→T	L120L (CTG→TTG)	bglJ →	DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,517	0	C	T	100.0%	125.6 / NA	39	L120L (CTG→TTG)	bglJ	DNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/23);  total (16/23)

CCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCT  >  NC_000913/4604273‑4604756                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGgag                                                                                                                                                                                                                                            <  1:70182/250‑1 (MQ=255)     accgttaCCAGTCAAGAGTCATTGCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGTAGAtttt                                                                                                                                                                                                                                       <  2:496399/246‑1 (MQ=255)            ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAg                                                                                                                                                                                                                                <  1:474981/251‑1 (MQ=255)              aGTCAGGAGTCATTTCAGTATGCCATGTCGCGGATCGCGTTTGGGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACCGAACTGGCGATTAAGGTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGa                                                                                                                                                                                                                              <  1:58293/251‑1 (MQ=255)                                          cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACg                                                                                                                                                                                                   <  2:25793/250‑1 (MQ=255)                                            cgggtctcGTTTGCGGTGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTCGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCa                                                                                                                                                                                                <  2:91764/247‑1 (MQ=255)                                                        gcgtcggTTATTTTTTCTTTTTCAGCTATGAGAAGTGAGGTCAGAGAGGGATTATCTGGTCTGCCTTAACTGGCGATTATGTCTTCGTGTACCCTGTGTTTAGTGATTGCGTATGATTATATTGAATCTTGGCTGATTGGGTCATTGTCGACATCACCGCTGGACGGTGTTTTAAGTAAATCGTCTACGTTGGAGATTTTTCATCATGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgacc                                                                                                                                                                                     <  2:432111/246‑1 (MQ=255)                                                           gcggTCATTTTTTATTTTTCTGCCATGAGAATTGAGCGCATAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAATTGTCCGCGTACCCGGCGTGTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTTTCGCTATCACCGCTGGACGGTGGATTAAGTAAAGCGGCAAAGTTGGAGAGTTTTCATCAGGAACTCTTTTTGTCATGAAATGGTGTACGTCATGCGACCGACCGACt                                                                                                                                                                                 <  2:496051/251‑1 (MQ=255)                                                                           tgccctacactacgctctccgatctagagGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTGTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTTAACAATCAGTGGTa                                                                                                                                                                  <  2:239796/225‑1 (MQ=255)                                                                                    tGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCa                                                                                                                                                         >  1:90921/1‑250 (MQ=255)                                                                                       gaAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGGCGCCATCACCGATGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGCCCGCCTGAACAATCAGTGGTACATTAACAAAAGc                                                                                                                                                     >  2:337075/1‑251 (MQ=255)                                                                                                    agagGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTa                                                                                                                                                                  >  1:239796/1‑225 (MQ=255)                                                                                                        ggATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGAc                                                                                                                                    >  1:160091/1‑251 (MQ=255)                                                                                                                  gCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGaa                                                                                                                          <  1:343943/251‑1 (MQ=255)                                                                                                                            aCTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCt                                                                                                                <  1:203604/251‑1 (MQ=255)                                                                                                                               ggCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCtt                                                                                                               <  1:248623/249‑1 (MQ=255)                                                                                                                                 cGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATg                                                                                                           >  1:338102/1‑251 (MQ=255)                                                                                                                                 cGATTAACTTTCCGAGTACACGGCGATTAGATATTGCGGATGCTGATATTGAAGCTCGGCTGCTTGGATCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAACGCGTCACCGTTGGAGATTTTTCACCAGGCACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGTCGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTAtt                                                                                                           >  2:70716/1‑250 (MQ=255)                                                                                                                                        gTTTCCGCGTTCCCGGCTTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCGCCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTg                                                                                                    <  1:318486/251‑1 (MQ=255)                                                                                                                                          ttCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAATCTCGGCTGATTGTTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTgg                                                                                                   <  1:305225/250‑1 (MQ=255)                                                                                                                                                gTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTc                                                                                             >  1:238643/1‑250 (MQ=255)                                                                                                                                                 tACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCCTTGTCGCCATCACCGCTGGACGGTGTATTACGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCACGTGGCTACTCa                                                                                            >  1:344201/1‑250 (MQ=255)                                                                                                                                                  aCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAt                                                                                          >  1:407763/1‑251 (MQ=255)                                                                                                                                                             aGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGCCTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTg                                                                               <  1:251777/251‑1 (MQ=255)                                                                                                                                                              gTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGc                                                                              >  1:43366/1‑251 (MQ=255)                                                                                                                                                                   tGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGag                                                                          <  1:283859/250‑1 (MQ=255)                                                                                                                                                                          gatgatATTGAATCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCATTGGTACATTAACCAAAGCCGGACGTTAATCCCGACGGAGAGAGAAATAGTGCTCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTa                                                                   <  2:445548/250‑1 (MQ=255)                                                                                                                                                                           atgatATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTaa                                                                  >  1:568935/1‑250 (MQ=255)                                                                                                                                                                                             tGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGAGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTACGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATc                                               >  1:231391/1‑251 (MQ=255)                                                                                                                                                                                                 tGGTTCATTTTCGCCCTCACCTCTGGAAGGTGTATTAAGTAATGCGGCAACTTTGGAGACTTTTCATCAGGACCTCTTTTTTTCATTAAATGGTGTCCGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCCTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAACCGaccctt                                            <  2:402243/250‑6 (MQ=255)                                                                                                                                                                                                     tCATTGTCGCCATGTCCGCTGGACGGTGTATTAAGGTAAGCGTTACTGTTGTCGCTTTGTTTTCATGAACTCTTTTGGACATTATATGTTGTACGGCAGTCGACCTTCTGACTGAACAATCAGTGTTACTTTAACCAAAGCCTGACGTTAAGCCCGACGTAGAGTGAAATCTTGCGCTTTAGGTCGCGGGGCTACTTAATTACACCCATTGCCGATCCGCTTAAACGCAATATCAAAACGATCCGTGcac                                        <  2:313023/250‑1 (MQ=255)                                                                                                                                                                                                              ccATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATCTTGCGCTTTATGTCGAGTGGCTACTCAATGACACAAATTGCCTAGCAGCTTAAACGCAATATAAAAACGATACGTGCACATAAATTTa                               >  2:533088/1‑250 (MQ=255)                                                                                                                                                                                                                     cGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGAGAtt                       >  2:343546/1‑250 (MQ=255)                                                                                                                                                                                                                        tGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCGTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTAATGTCg                    >  2:507963/1‑251 (MQ=255)                                                                                                                                                                                                                         ggACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTc                     <  1:472528/249‑1 (MQ=255)                                                                                                                                                                                                                                 tATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTggac           >  2:131491/1‑249 (MQ=255)                                                                                                                                                                                                                                  aTTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCGTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCg          <  1:507963/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaaGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCAGTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGta    <  2:157750/250‑2 (MQ=255)                                                                                                                                                                                                                                          aaGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt  <  1:337075/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCT  >  NC_000913/4604273‑4604756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑