Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,280	C→T	E99K (GAG→AAG)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,280	0	C	T	100.0%	100.9 / NA	34	E99K (GAG→AAG)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/23);  total (11/23)

GTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAA  >  NC_000913/4571065‑4571499                                                                                                                                                                                                                         |                                                                                                                                                                                                                             gTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTTCTCTCGCCATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGGTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTTAGGCTGGGGGCTTCGACATCCGGTGTGCTTTTGCTTAACGGGACAATGGAACTGTCGCTATTCGCCTTCAGAATATCCAGCACCTGATCCCACTGGGTAAttt                                                                                                                                                                                           >  1:15488/1‑251 (MQ=255)      cggctggacccaccGGGCCGCTGGCAAGCAACAGGCGGGCAATCTGCCCTCGCAATTCCTGATGACCTGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCAGGCTTAGCGCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCAcc                                                                                                                                                                                      <  2:570516/242‑1 (MQ=255)      cggcggTGACCCCCGCGCCGCTTGCGAGCAGCAGGGGGGAAACCGGCTCCCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTGGAAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCAc                                                                                                                                                                                       <  1:209645/250‑1 (MQ=255)         gggggcccaccGAGCGGCTGTAGAGCATCAGGCGGGAAATCTGCTCTCGCAATACCCGCTGACCGGTTAACATG‑TCATAACCGAGAATGTGTTGCAGATTATGCTGCACACCCCGGCTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCggg                                                                                                                                                                                   <  1:125158/245‑1 (MQ=255)           gTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGgcg                                                                                                                                                                                 >  1:182387/1‑251 (MQ=255)             gACCACCGAGCCTCTGTCGAGCATCAGGCGGGCAATCTGCTCACGCAATACTCGCTGACCGGCTAACAAT‑GCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCt                                                                                                                                                                               <  1:530908/251‑1 (MQ=255)                ccccagcgcgcgggcgggCATCAGGCGGGAAAGCTGTTCCCCAAATACTCTCTGACCGGCTAACAAG‑TCATACCCGAGAACGGTTTGACGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCa                                                                                                                                                                            <  2:540262/234‑1 (MQ=255)                   cGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCCGCACCTGATCCCACTGGGTAATTTCCACCGGACGCAGCACCg                                                                                                                                                                         >  2:96004/1‑251 (MQ=255)                       ccGCTGTCGAGCATCCGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATACAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGAcg                                                                                                                                                                     >  1:431209/1‑251 (MQ=255)                           tGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCCTAACCGAGAACGGTTTGCAGATTATGCTGCACCACCAGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAAAAATGGAACTGTAGCTATGCGCCTTCAGCATATCCAGCAAATGATCCCACTGGGTAATTTACACCGGGCGCTGCACCGGACGCGTc                                                                                                                                                                 >  1:365125/1‑251 (MQ=255)                             tCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCAt                                                                                                                                                               >  1:61389/1‑251 (MQ=255)                                  ccgcgggtgggCAATCTGTCCGCGCAATCCCCGCTCTCCGGCTAAAAAG‑CCATTCCCGAAACCGGTTTGCTGATGATGCTGCCCCCCCCGGCTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATcggcg                                                                                                                                                          <  2:307729/243‑1 (MQ=255)                                    tCAGGCGGGCAATCTGCTCTCGCAATCCTAGATGACCGGCTAACAAG‑TCATAACCGCGAACGGTTTGCAGATTAAGCTGCACCACCCGGCTTAACTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCATTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCAACGGCCACGTCATcagcggg                                                                                                                                                        >  2:551486/1‑250 (MQ=255)                                      aGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTAc                                                                                                                                                      <  1:57452/251‑1 (MQ=255)                                                   tctctCGCAATCCTCGCTGACCGGCTAACAAG‑TCATAACCGAGACCGGTTTGCAGATTATGCTGCACCCCCCGCCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑Tggg                                                                                                                                          <  1:187268/248‑1 (MQ=255)                                                     tctcGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACCGGCGGG‑Tgggggtt                                                                                                                                      >  1:429054/1‑247 (MQ=255)                                                      ctcGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTtgcgggggggggttt                                                                                                                                      >  1:116538/1‑241 (MQ=255)                                                      cgcgaaagcacggCTGACCGGATAA‑AAGATCATAACCGAAAAGGTTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGGTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCtt                                                                                                                                       <  2:518016/237‑1 (MQ=255)                                                           aaTACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGCACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATcggcggaaccgtgcgcagggggttgcgcgt                                                                                                                                 >  2:423200/1‑227 (MQ=255)                                                           aaTACCCGCTGCCCGGCTAACAAG‑TCATAACCGGGAAGGGTTGGCAGATTATGCTGCACCCCCCGGTTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTtg                                                                                                                                <  1:122425/251‑1 (MQ=255)                                                                               cAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTTGCGGCCTGGG‑TTTATGTTGTGGGGCAAAAAAAACCGAAc                                                                                                            >  1:434109/1‑251 (MQ=255)                                                                                          ccGAGAACGGTTTGCAGTTTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTGCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCgg                                                                                                  <  1:356829/251‑1 (MQ=255)                                                                                              gaACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑Tg                                                                                                                                            <  1:347779/205‑1 (MQ=255)                                                                                                 cGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAAccc                                                                                                                <  1:203341/230‑1 (MQ=255)                                                                                                            ttaagcggcACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACTCTGCTGCGGAGTGATGAGCTTCATCg                                                                                 <  1:493067/243‑1 (MQ=255)                                                                                                                     cccaccaGGCCTAGCTGCCGCCACAGCGGTGTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGt                                                                       <  1:544931/250‑1 (MQ=255)                                                                                                                                 accccccGCCACAGCGGTTTCAGGCAGGGCGCTTCGACACCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCct                                                            <  1:204789/246‑1 (MQ=255)                                                                                                                                    ccactccccaGCGTTTTCAGTCTGGGCGCGCCGCCATCCGGTTTGTTTTCGCTTTACGCAACAATGGAACTGTTCCGATGCTCCATCAGCATTCCCAGCACCTGATCCCACTGTGTAATTTCCATCGGGCGCGTCATCGGTCGCGGCACCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGCGAGTCAAAATAACCCGTACTAGGCTGTGGCGTGAGGCGCTGCATCTTCTCCAGCGTCTGACACgatggctg                                                        <  2:514039/243‑8 (MQ=255)                                                                                                                                     cccGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCtgctgc                                                       <  1:569856/251‑1 (MQ=255)                                                                                                                                      ccGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCGTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCa                                                      <  1:228619/251‑1 (MQ=255)                                                                                                                                                       ggCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACg                                     <  1:423200/251‑1 (MQ=255)                                                                                                                                                            ggCGCTTCGGCATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTTGCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTGCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTg                                <  2:194626/251‑1 (MQ=255)                                                                                                                                                                                gCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACGGGGTAAGTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACGGGCGGC‑TGGGCTTGACGTTGTGCGACAAAATAACCCGAACGCGGCTGGGGAGTGATGGGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc             <  1:364163/250‑1 (MQ=255)                                                                                                                                                                                          aaCGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa  <  1:111235/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                             GTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAG‑TCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGC‑TGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAA  >  NC_000913/4571065‑4571499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑