Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,499	C→T	intergenic (‑93/‑124)	idnD ← / → idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,499	0	C	T	100.0%	112.4 / NA	36	intergenic (‑93/‑124)	idnD/idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

TTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCC  >  NC_000913/4494255‑4494742                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    tttCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTa                                                                                                                                                                                                                                                 <  1:290841/249‑1 (MQ=255)               ataatGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAAc                                                                                                                                                                                                                                 >  1:464997/1‑251 (MQ=255)               ataatGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAAc                                                                                                                                                                                                                                 >  1:311892/1‑251 (MQ=255)                       aaTCGGAACCGCAAAGTCCACCTCGGGTTATGTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCTGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGAtgtg                                                                                                                                                                                                                         <  1:219311/251‑1 (MQ=255)                                    aaTTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCtt                                                                                                                                                                                                            >  2:238929/1‑251 (MQ=255)                                    aaTTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTAGTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCtt                                                                                                                                                                                                            <  2:174602/251‑1 (MQ=255)                                     aTTCCACCGCTGGGTATTTGTACTGATGTCCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGGGTTTTCACTTGCATAAATTCCTCACTTTTCTTGTAGACATCTCTGAAGCATTTAATCGGGTTTAGCGGTCATTATTTGTTATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTa                                                                                                                                                                                                           <  1:534314/251‑1 (MQ=255)                                           ccTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGt                                                                                                                                                                                                      >  1:58134/1‑250 (MQ=255)                                              cGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGTTTTTAACTGTCATTATTTGTGATGAAGATCACGGCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGa                                                                                                                                                                                                   <  2:147850/250‑1 (MQ=255)                                                     gttctacgtatttaccATTTTTACTCCAATCTACCCTCTGCTCGGTTACGGCAACATGTTTCTTTTCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCGCTCTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTATCTGTCAATATTTTTTATGAAGATCACGTCAGTAAAGTGTTATATTATTATGTTACGCATAACGTGATTTGCCGTGTTATTCTTATCAGTAGAAATaaaa                                                                                                                                                                                            <  2:311700/236‑1 (MQ=255)                                                        gTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAACGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc                                                                                                                                                                                        <  1:69940/251‑1 (MQ=255)                                                                gtgccATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                                                                                                                                                                <  1:190501/248‑1 (MQ=255)                                                                 ttCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                                                                                                                                                                >  1:371596/1‑250 (MQ=255)                                                                  tCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatta                                                                                                                                                                               <  1:374370/250‑1 (MQ=255)                                                                             cccAATCTATCGGCTGCTCTGGAACGGCAAAAGTTGTCTTGCCCTCAACAACGCAGGACTTTGTGTTCAATTGCATAAATTCCTCTCTGGTCAGTTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCTCGTCAGAAAATTGTTATATTACTAAGTTACGCATAACGTGATGTGCCTTGTAAATCTTATCAGGAGAAATAAAAAAACGTGAAATtattatttcgccaa                                                                                                                                                                   <  2:71436/250‑9 (MQ=255)                                                                              ccAACCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGAGCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAg                                                                                                                                                                   <  2:235901/250‑1 (MQ=255)                                                                               cAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTAGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGACATTATTATGCCGCCAGGc                                                                                                                                                                 >  2:116673/1‑251 (MQ=255)                                                                                                        cAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAgatgct                                                                                                                                        >  1:228212/1‑249 (MQ=255)                                                                                                                    tGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATGCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGAtt                                                                                                                              <  2:464997/249‑1 (MQ=255)                                                                                                                                    ggACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAg                                                                                                            <  1:559255/251‑1 (MQ=255)                                                                                                                                           gtgttCACTTTCATAAATTCCTCACTGTTCAGGTTGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTGGTTATGAAGAGTACGTCAGAAAATTGTTATATTAAAATGTTACGCATAACGTGATGTGCATTGTAATTCTTATCAGTAGAAAGAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGTAGGTAAGAGGATTCAGTAGATTTTAAATGGCGGGTTAAAGCTTTAtt                                                                                                     <  2:376424/248‑1 (MQ=255)                                                                                                                                                           aTTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAgg                                                                                                                                                 <  2:539997/191‑1 (MQ=255)                                                                                                                                                           aTTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAgg                                                                                                                                                 >  1:539997/1‑191 (MQ=255)                                                                                                                                                                      gTCAGGTAGACACCTCGAAAGCATTAAAGAGGTTTTACCTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATACCGTGCCGTGCATTGTAATTCTTATCAGTAGAAATAAAAACACGGGAAATTATTATGCCACAAGACGTAGTATCGAAACAGGAAAAATGATGCAGGAGCTTGTTAATGGCGGGGGAAAGCATTATTTTGCTGGGCGtgtgcggggagggaaa                                                                           >  2:504532/1‑235 (MQ=255)                                                                                                                                                                             aGACACCTCGGAAGCATTTAAGCGGTTTTAAATGTCATTATTTGTGATGAAGATCACGTCAGAACATTGTTATATTACTATGTTAAGCATAACGAGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCAGCCAGGCGTAGTATCGCAGCAGGAAAGATGATTAAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATTGGCGTTTCGGGGGGTGGGaaaccaata                                                                    >  2:507612/1‑244 (MQ=255)                                                                                                                                                                                cacCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTg                                                                <  1:110859/251‑1 (MQ=255)                                                                                                                                                                                     cGGAAGCATTTAAGCGGTTTGAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTGATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGc                                                           <  1:430545/251‑1 (MQ=255)                                                                                                                                                                                       gAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCa                                                          >  1:87936/1‑250 (MQ=255)                                                                                                                                                                                             tttAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTAATATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAACTAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGGAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGAAGGAAGGTTGc                                                   >  1:285142/1‑251 (MQ=255)                                                                                                                                                                                                              gTCATGATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATTTTACGCATAACGTTATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGTTGAAATCTTTATTTTGATGTGCTTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGc                                    <  2:62940/249‑1 (MQ=255)                                                                                                                                                                                                                 attattTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAAt                               <  1:238929/251‑1 (MQ=255)                                                                                                                                                                                                                       tgtgATGAAGATCACGTCAGAAAATTATTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATGATTATGCCTCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTAtt                          <  2:96384/250‑1 (MQ=255)                                                                                                                                                                                                                             gaAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCg                                                                                           >  1:120299/1‑179 (MQ=255)                                                                                                                                                                                                                             gaAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCg                                                                                           <  2:120299/179‑1 (MQ=255)                                                                                                                                                                                                                                         aGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATc       >  1:68246/1‑251 (MQ=255)                                                                                                                                                                                                                                               ttGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCTGcc  >  1:312967/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCC  >  NC_000913/4494255‑4494742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑