Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,487,022 C→T R269C (CGT→TGT)  lptF → lipopolysaccharide transport system protein LptF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,487,0220CT95.5% 315.8 / NA 110R269C (CGT→TGT) lptFlipopolysaccharide transport system protein LptF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/55);  minor base G (3/2);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACA  >  NC_000913/4486776‑4487265
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     
gtggtggtgGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGGTGCGCTCGACCCGAACGATACCAACCAGATGGACATGCGCACATTGTGGAAACCTGACACCGATCGTGCGCGCGCAGAACTGAACTGGGGTAt                                                                                                                                                                                                                                                   >  2:473505/1‑251 (MQ=255)
    gggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCGCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑g                                                                                                                                                                                                                                             <  1:503277/250‑1 (MQ=255)
      gtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑Gtt                                                                                                                                                                                                                                           >  1:470739/1‑251 (MQ=255)
        ggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTgg                                                                                                                                                                                                                                         >  1:514279/1‑251 (MQ=255)
            gATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTAtt                                                                                                                                                                                                                                     >  1:304273/1‑251 (MQ=255)
            gATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGAAACCGACCAGATGGACATGCGAACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAAATGAACTGGTGTATC‑AC‑TTTGGTAtt                                                                                                                                                                                                                                     >  2:565228/1‑251 (MQ=255)
            gATTCCGGACATGTAACCCAGCTGCGCGACGGCGCCCATGGCGTCACTCTCAACCAGGGAACGCGCGTCGAAGGCTCTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGAGTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACGGACACCGATCGTTCTCGCGCAGAACTTAACTGGTGTATC‑AC‑GTTGGTa                                                                                                                                                                                                                                       <  2:291710/249‑1 (MQ=255)
            gATTCCGGACATCTAACAACGCTGCGCGACTGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTAGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGAATTCCAGGATTATCAGGCGATCAT‑GTTGCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACTAGATGGAAATGCGCACATTGTGTAACACTGACTCCGGTCGTGCTCGCGCAGAACTTAACTGGTGTATC‑AC‑GTTGGTAtt                                                                                                                                                                                                                                     <  2:388783/251‑1 (MQ=255)
             aTTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GATGGTATTc                                                                                                                                                                                                                                    >  1:300066/1‑251 (MQ=255)
               tCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGGCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCAGTACGGACGTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCa                                                                                                                                                                                                                                   <  2:211855/250‑1 (MQ=255)
                 cGGACCTTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGCCCCGAACGATACCGACCAGATGGACATGCGCACATTGGGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAAATGGTGTATC‑AC‑GTTGGTATTCACCg                                                                                                                                                                                                                                >  1:363091/1‑251 (MQ=255)
                   gACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCTCCgtg                                                                                                                                                                                                                              <  2:520764/251‑1 (MQ=255)
                    aCATTTAACCCAGCTGCGAGCCGGCGCCCAGGTCGTCCCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCgtgt                                                                                                                                                                                                                             <  2:347348/251‑1 (MQ=255)
                      aTTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGCTCATTGGT‑CACCAGGCGGTGGCGCTCGCCCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGCAACCGCTCGTGCTCGCGCAGAACTGAACTGGTGTATC‑CC‑GTTGGTATTCACCGTGttt                                                                                                                                                                                                                           >  2:520893/1‑251 (MQ=255)
                      aTTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTAGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGttt                                                                                                                                                                                                                           <  1:571804/251‑1 (MQ=255)
                         tAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTat                                                                                                                                                                                                                         <  2:214145/250‑1 (MQ=255)
                                  tGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGACCTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACtt                                                                                                                                                                                                               >  2:169482/1‑251 (MQ=255)
                                   gcgcGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTGCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTGTATGATGGCACTTa                                                                                                                                                                                                              <  2:104223/251‑1 (MQ=255)
                                   gcgcGACGGCTCCCAGGTCGGCACTCTCAACCAGGGAACGCGCGTCGAAGGCACTTCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTa                                                                                                                                                                                                              <  2:14570/251‑1 (MQ=255)
                                       gacggtccccAGGGCGTCACTCTCAACCAGGGAGCCCGGTTCGTAGGTACTGCAATGTTACGTGATTTACGCATTACGGACTGCCAGGATTTGCAGGCGATCATTGGT‑CACCAGGCGGGGGCGCTCGACCCGAACGATACCGTGCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGGGTATC‑AC‑GTTGGTATTCTCCGTGTTTATGATGGCACTTATGGt                                                                                                                                                                                                          <  2:306478/244‑1 (MQ=255)
                                              cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTAc                                                                                                                                                                                                     <  1:565228/249‑1 (MQ=255)
                                                   gtcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTg                                                                                                                                                                                                >  2:545258/1‑249 (MQ=255)
                                                   gtcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAg                                                                                                                                                                                              <  1:263898/251‑1 (MQ=255)
                                                      gtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGt                                                                                                                                                                                           >  1:337076/1‑251 (MQ=255)
                                                          cgctcatccagggacgtcgcTTGGAAGGCCCTGCAATGTTATGTGAGTGCCGCAGTACGGACTTCCAGGACTAGCAGGGGATCAAGGGT‑CACCAGGCGGGGGCGCTCGACCCGAACGATACCGGCCAGATGGACATGCGCACATTGTGGAACACTGTGACCGATCTTGCTAGGGCAGAGCAGAACTGGTGGAGC‑GC‑GTTGGTATGGTCCGGTTTTATGATGGCACTTATGGTCGTATCGCTGTGGGTGGtt                                                                                                                                                                                       <  2:258993/234‑1 (MQ=255)
                                                                   aGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCGCTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGt                                                                                                                                                                              >  1:333372/1‑251 (MQ=255)
                                                                    gggAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTc                                                                                                                                                                             >  1:14780/1‑251 (MQ=255)
                                                                      gcaCGCGCTTCGAAGGCACTGCAATGATACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGACCGATACCGACCAGCTGGACATGCGCACATTGTGGAACACTTACCCCGCTCGGGCTCGCGAAGAACTGAAATGGTGTATC‑AC‑GTTGGTCTTCACCGTGTTTATGATGGCACTTATGGGCGTACCGCTTAGCGTGGTTAACCCACGTCa                                                                                                                                                                            >  2:466229/3‑250 (MQ=255)
                                                                      gAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATGACGGACTTCCAGGATTATCAGGCGAGCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAg                                                                                                                                                                           <  2:118784/251‑1 (MQ=255)
                                                                       aaCGCGCTTCGAAGGCACTGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAAAGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTAGGAATTCCCCGTGTTTATGATGGCCCTTATGGTCGTCCCGCTGAGCGTGGTTAACCCACGTCAgg                                                                                                                                                                          >  2:120171/1‑251 (MQ=255)
                                                                         cgcgCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAggg                                                                                                                                                                         >  1:535071/1‑250 (MQ=255)
                                                                          gtgctgcgaaggcactgaaagtttACGGGAGTTCCGCATTACGGACTGCCAGGATTATCAGGGGATCATTGGT‑CAACAGGCGGTGGCGCTCGACCCGAACGACACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCTCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCTTGTTGATGATGGCTCTGATGGTCGTACCGCTGAGCGTGGTTAACCCACGTAAGGGAc                                                                                                                                                                       <  2:21476/229‑1 (MQ=255)
                                                                           cgcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGtt                                                                                                                                                                                       >  2:284270/1‑234 (MQ=255)
                                                                           cgcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGtt                                                                                                                                                                                       <  1:284270/234‑1 (MQ=255)
                                                                            gcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGCTTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGAATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGaagc                                                                                                                                                                     >  2:561688/1‑248 (MQ=255)
                                                                            gcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGAcgc                                                                                                                                                                     >  1:278218/1‑251 (MQ=255)
                                                                            gcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGGGTTTATGATGGCACTTATGGTAGTACCGCTGAGCGTGGTTAAACCACGTCAGGGCcgc                                                                                                                                                                     >  2:306351/1‑251 (MQ=255)
                                                                               tCGAAGGCACTGCAATGTTTCGTGATTTCCGCATTACGGACTGCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACAAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGAcgcg                                                                                                                                                                    <  2:23975/249‑1 (MQ=255)
                                                                               tCGAAGGAACTGCAATGTTACGTGATTTCCGCATTACGGCCTTCCAGGATTATCAGGCGATCATTGGT‑CCCCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACGTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGAcgcg                                                                                                                                                                    <  2:476880/249‑1 (MQ=255)
                                                                                cGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATACGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTAc                                                                                                                                                                 >  1:375709/1‑251 (MQ=255)
                                                                                  aaGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTg                                                                                                                                                               >  1:14429/1‑251 (MQ=255)
                                                                                   aGGCACTGCACTGTTACGAGATTTCCGCATTACGGACTTCCAGGATTATCCGGCGCTCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGCTCGTGCACGCGCAGAACTGAACTGGGGTATC‑AC‑GTTGGAATTCACCGTGTTTGTGATGGCACTTCTGGTCGTACCGCTGAGCGAGGTTAACCCACGTCAGGGCCGCGTACTGt                                                                                                                                                              >  2:173540/1‑251 (MQ=255)
                                                                                    ggCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTc                                                                                                                                                             >  1:66787/1‑251 (MQ=255)
                                                                                          gcaatGTTACGTGTGTGCCGAATTACGGACTTCTAGGATTATCAGGCGATCATTTGT‑CACCCGGCTGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGTAACACTGACACCGATCTTGACCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTTGTACCGCTTAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAtgct                                                                                                                                                       <  2:130211/247‑1 (MQ=255)
                                                                                            aatgctACGTGATTTCCGCATTACGGCCTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGTGTACTGTCGAtgctgc                                                                                                                                                     <  1:468402/246‑1 (MQ=255)
                                                                                               gTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACCTTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTgcc                                                                                                                                                    >  1:261436/1‑249 (MQ=255)
                                                                                                ttACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGAGGCACACTGACACCGCTCGTGCTCGCGCCGAACTGAACTGGTGTATC‑AC‑GTTGGAATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCAACGTCAGGGGCGCGTACTGTCAATGCTgccagc                                                                                                                                                 >  1:291053/1‑251 (MQ=255)
                                                                                                  aCGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTgccagcca                                                                                                                                               <  1:116251/251‑1 (MQ=255)
                                                                                                     ggcttttcGCATTACGGACTTCCAGTATTCGCAGTCGATCATGGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACTCCGATCGGGCTCGCGCAGAACTGAACTGGTGTATA‑AC‑GTTGGTATTCCCCTTGTTTATGATGGCACTTATGGTCGTACCGCTGATCGTGGTTAACCCACGTCATGGACGCGTACTGTCGATGCTGCTAGCCAt                                                                                                                                              <  2:390719/242‑1 (MQ=255)
                                                                                                      gatttccgactgtcgggcTTCCAGGATTATCAGGCGAACATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCAtgct                                                                                                                                           <  2:502832/234‑1 (MQ=255)
                                                                                                      gATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCAGTGGT‑CACCAGGCGGTGGCGCGCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCAtgct                                                                                                                                           <  2:304273/251‑1 (MQ=255)
                                                                                                       aTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCAtgctg                                                                                                                                          <  1:284068/251‑1 (MQ=255)
                                                                                                            cGCATTAAGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCAACCCGCACGCTACCGACCAGCTGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGACCTGGTGTATC‑AC‑GTTGGTATTAACCGTGTTTCTGATGGCACTTATGGTCGTACCGATGCGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATTCTGCTGtt                                                                                                                                     >  2:239586/1‑250 (MQ=255)
                                                                                                             gCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTAt                                                                                                                                    <  1:519920/251‑1 (MQ=255)
                                                                                                              cATTACGGACTTCAAGAATTATCAGGCGATCATTGCT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAGCACTGACACCGATCGTGCTCGCGCATAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGTACTTATGGTCGTACCGCTGGTCGTGGTTAACACACGTCAGGGACGCGTACTGTCACTGCTTCCAGCCATGCTGCTGtctc                                                                                                                                   >  2:139782/1‑248 (MQ=255)
                                                                                                                  aCGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTAc                                                                                                                                <  1:560582/250‑1 (MQ=255)
                                                                                                                  aCGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTAc                                                                                                                                >  2:560582/1‑250 (MQ=255)
                                                                                                                    ggACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACttt                                                                                                                             >  1:410444/1‑251 (MQ=255)
                                                                                                                         tCCAGGATTATCAGGCGATCATTGGT‑CACCAGGAGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTCTTCACCGTGTTTATGATGGGACTTATGGTCATACCGCTGAGCGTGGTTAACCCACGGCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCGGTATCTAATTttcttc                                                                                                                        >  2:486302/1‑251 (MQ=255)
                                                                                                                         tCCAGGATTATAAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTttcttc                                                                                                                        >  2:279624/1‑251 (MQ=255)
                                                                                                                          ccAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTcc                                                                                                                       <  1:148787/251‑1 (MQ=255)
                                                                                                                             ggATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTg                                                                                                                     >  1:339325/1‑250 (MQ=255)
                                                                                                                                 tATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATcc                                                                                                                 >  1:41/1‑250 (MQ=255)
                                                                                                                                  aTCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATcc                                                                                                                 >  1:239189/1‑249 (MQ=255)
                                                                                                                                  aTCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCa                                                                                                                <  1:242239/250‑1 (MQ=255)
                                                                                                                                  aTCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAg                                                                                                               <  1:367420/251‑1 (MQ=255)
                                                                                                                                  aTCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTT‑CTTCCTGATcccg                                                                                                               >  1:556739/1‑248 (MQ=255)
                                                                                                                                            cATTGGT‑CCCCATGCGGGGGCGCTCGACTCGAACGATATCGCCCATAGGGACATGCGCACATTGGGGAACGCGGACACCTATCGTGCTTGCGCAGAACTGATCATGTTTAGC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCCCTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGTACGCGTACTGTCGATGCGGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTTCCTGa                                                                                                     <  2:529536/251‑1 (MQ=255)
                                                                                                                                                agggctccAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGAGGGACATGCGCACATTGTGGAACACTTATACCTATCGTGCTTGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCTCTTAGCGTGGTTAACCCACGTCAGGGATGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAAt                                                                                                  <  2:300066/245‑1 (MQ=255)
                                                                                                                                                      ccAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGaccg                                                                                            <  2:484013/251‑4 (MQ=255)
                                                                                                                                                       cAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAAcgg                                                                                           <  1:209843/251‑1 (MQ=255)
                                                                                                                                                            ggTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGt                                                                                       >  1:535500/1‑250 (MQ=255)
                                                                                                                                                                      gACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCt                                                                            <  1:342592/251‑1 (MQ=255)
                                                                                                                                                                           gAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGcagggcc                                                                       >  1:551586/1‑245 (MQ=255)
                                                                                                                                                                           gAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGAcc                                                                       <  1:188412/251‑1 (MQ=255)
                                                                                                                                                                              cGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGCTCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAgtgaacgtgggcccac                                                                    >  2:552663/1‑236 (MQ=255)
                                                                                                                                                                                    cGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTACCCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAAGGAAGCTGGACCCGCCGCtgg                                                              >  1:357044/1‑250 (MQ=255)
                                                                                                                                                                                     gACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTaaaaggtaggtagacccgccgccggg                                                             >  2:330136/1‑228 (MQ=255)
                                                                                                                                                                                     gACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCtgtg                                                             <  1:406112/251‑1 (MQ=255)
                                                                                                                                                                                      aCCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGAAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCACTGAAATCGAACGGCGGGAAAGGAAAGGTgggaccggcactttgg                                                            >  2:460641/1‑237 (MQ=255)
                                                                                                                                                                                       ccAGATGGACATGCGCACAGTGTTGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCTTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCtgtgga                                                           <  1:366643/251‑1 (MQ=255)
                                                                                                                                                                                        cAGATGGACAGGCGCAAAGTGTGGAACACTGAAAACGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTTAGGGACGCGTACTGTGGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGAGCTAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCGGGACCCGACGCtgtggat                                                          <  1:239586/251‑1 (MQ=255)
                                                                                                                                                                                         aGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCtgtggatg                                                         <  1:545258/251‑1 (MQ=255)
                                                                                                                                                                                             ggACATTCGCACATTGTGGAACTCTGACCCCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCCCTTTTGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCGGCTGTATCTACTTTTCTGCCTGATCCAGACCTCCCTGAAATCGAACGTCGGTAAAGGTAAGCTGGACCCGACGCtgtggatgtgg                                                      <  2:246955/250‑1 (MQ=255)
                                                                                                                                                                                              gccaggcggcactggtgGAACACTGACTCCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTAATCACCGTGTTTATGATGGCACTTATGGTCGTCCCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGAc                                                    <  2:134262/237‑1 (MQ=255)
                                                                                                                                                                                               aCATGCGCACATTGTGGAACACTGACACCGATCGTGTTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGAcc                                                   <  1:488771/251‑1 (MQ=255)
                                                                                                                                                                                                   gcgcACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTa                                               <  1:286392/251‑1 (MQ=255)
                                                                                                                                                                                                     gaacaTTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAAc                                             <  1:295675/249‑1 (MQ=255)
                                                                                                                                                                                                       acaTTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCt                                           <  1:242232/251‑1 (MQ=255)
                                                                                                                                                                                                              ggAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTa                                     <  1:454676/250‑1 (MQ=255)
                                                                                                                                                                                                                      gcaccctaccgtgcctcggacgaactgaactGGTGTGCT‑AC‑GTTGGTATTCACCTGGTTTATGATGGCATTTATGGGGGTTCCGCTGAGCGTTGTTAACCCACGTCAGGGACGCGTACTGTTGATGCTGCCAGCCATGCTGCTGTACCTACTTTTCGTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGTTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCt                              <  2:268732/228‑1 (MQ=255)
                                                                                                                                                                                                                      gACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCtt                             <  1:165939/250‑1 (MQ=255)
                                                                                                                                                                                                                      gACACCGAGCGTGCTCGCGCATAACTGAACTGGTGTAGC‑GC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCttt                            <  1:521875/251‑1 (MQ=255)
                                                                                                                                                                                                                      gACACCGACCGTGCCTGCGCAGAACTTAACGGGTGGA‑C‑ACCGTTGGTATTCACCGCGTTTATGAGGGCTCTTATGGTCGTACCGCTGAGCGGGGTTAACCCACGTCAGGGACGCGTACTGGCGATGCTGCCAGCCATGCTGCTGTATCTACTTTCCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCGGGACCCGACGCTGTGTATGTGGACCGTTAACCTGATTTATCTGGCtt                             <  2:41/250‑1 (MQ=255)
                                                                                                                                                                                                                        ccccGATCGTGCTCGCGCAGACCTGAAC‑GGGGTTTCCAC‑GTTGTTATTCACCGTGTTTAGGAGGGCACTGATGGTCGTACCGCTGGGCGTGGTTACCCCCCGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCT‑CTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGc                         <  2:556739/249‑1 (MQ=255)
                                                                                                                                                                                                                          ccGATCGTGCTCGCGCAGAACTTAAATGGTGTTAC‑AC‑GTTGGTATTAACCGTGTTTATGATGGCACTTATGGTCGTACCGTCGAGCGTGGGTAACCCACGTCAGGGACGCCTCCTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTACCTGCTCCAGACCGCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGAGTTATCTGGCTTTAGCg                        <  2:279907/251‑1 (MQ=255)
                                                                                                                                                                                                                            gATCGTGCTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGGAAGATGGACCCCACGCTGTGGATGTGGGCCGTTAACCTTATTTATCTGGCTTTAGCGAt                      >  1:478233/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cTCGCGCAGAACTGAACTGGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGGAAGCTGGACCCGACGCTGTGGGTGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGttttc               >  1:161365/1‑248 (MQ=255)
                                                                                                                                                                                                                                          ataactgaactgGTGTATC‑TC‑GTTGGTATTCCCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGCGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCttt        <  2:565723/249‑1 (MQ=255)
                                                                                                                                                                                                                                                gaactgGTGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTCCCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGGGGATGTGGACCGGTAACCTGATTTATCTGGCTTTAGCGATTGTTATCAACCTTTGGGacc  >  1:62737/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     
GTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATC‑AC‑GTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACA  >  NC_000913/4486776‑4487265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑