Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,486,869 T→A L218M (TTG→ATG)  lptF → lipopolysaccharide transport system protein LptF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,486,8690TA97.6% 405.3 / NA 129L218M (TTG→ATG) lptFlipopolysaccharide transport system protein LptF
Reads supporting (aligned to +/- strand):  ref base T (1/1);  major base A (62/62);  minor base C (3/0);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

GAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTC  >  NC_000913/4486622‑4487110
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  
 aaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGtt                                                                                                                                                                                                                                                >  1:484398/1‑251 (MQ=255)
   agcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGGGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCACCCAGGGAACGCGCTTCGAAGGCCCTGCAATGTTAc                                                                                                                                                                                                                                              >  2:263898/1‑251 (MQ=255)
       aaCCCTGGCATGGCGGCGCTGGCGATATGGCAATTCCAGCAAGCGACTAATGGCAGCTTGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGAGGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCTAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGTCACTGCTATGTTAc                                                                                                                                                                                                                                              <  2:306957/247‑1 (MQ=255)
           cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCCGCCAGCGACTAACAGCAGCTCGGTGCTGTGCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGAGCAAAATCGACCAAAAGGGAA‑TGCAAGTCCTTCTGTGGGGGTGGCCGATTCCGGACATTTAACCCAGCTGCGTGGCGGATCCCAGGCTGTCCCTCTCAACCAGGGTACGCGCTGCGAAGGAACTGCAATGTTTCGTGATTTc                                                                                                                                                                                                                                      >  2:58368/1‑251 (MQ=255)
             tgCATGGCTGTGCTGGCACAAGGGCAATTCCAGCATGCGACTAATGGCAGCTCGGTGCTGTTCCTCGAAAGC‑GTTGACTGCATCGATTTCAAAGATTT‑TTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCCTCTGTGGTGGTGGCCGATTCCTGACATTTAACCCAGCTGCGCGACGGCTCCCATGTCGTCACTCTCAACCAGGGAACGCTCTTCGAAGGCACTGCAATGTTACGTGATTTc                                                                                                                                                                                                                                      <  2:157730/248‑1 (MQ=255)
               cATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAAAGGCAGCTCGGTGCTGTTCATCGAAAGC‑GGTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCCAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTTACCCAGCTGCGCGGCGGCCCCCCGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTCCGTGTTTTCCgat                                                                                                                                                                                                                                  >  2:242239/1‑249 (MQ=255)
               atggcggggcagggCGCA‑GGGCAATTCCAGCAAGCCGCTTATGGCAACTCGGTGCCTTTCAACGAAAGC‑GTTTAAGGGCGCGATTTTCAAGATGT‑GTTCCTTGCGCAAATTAGGACAAAAGGTAA‑TGCCCCTCCTTCTTTGGTGGTGGCCGATTCCGGACATTTAACCCAGGTGCGCGACGGCTTCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCAt                                                                                                                                                                                                                                 <  2:406390/239‑1 (MQ=255)
                      gcgttggagcAAGGGTAATTCCATCTAGCGACTAATGGCAGCTTGTTGCGGTTCAGCGAAAGC‑GTTGTCGGCAGCGATTTCAAAGA‑GTAGTCCCTCGCGCAAATTCTACTCAAAGGTTA‑TGCACGTCCGTCTGTGGTGGTGGCCGATGCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCTGTCTCATCCAGGGAACGCGCTTCGAAGGAACTGCAATGTTACGTGATTTCCGCATTACGGa                                                                                                                                                                                                                           <  2:465398/243‑1 (MQ=255)
                      gcgcTGGCGCAAGGGCAATTCCAGCAAGCGGCTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATGTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGa                                                                                                                                                                                                                           <  2:3881/251‑1 (MQ=255)
                       cgcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGAc                                                                                                                                                                                                                          >  1:211855/1‑251 (MQ=255)
                       cgcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATAGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGTCACTGCAATGTTACGTGATTTCCGCATTACGGAc                                                                                                                                                                                                                          >  1:130211/1‑251 (MQ=255)
                        gcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACt                                                                                                                                                                                                                         >  1:388783/1‑251 (MQ=255)
                          tGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAACGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCAGAATTACGGACTTc                                                                                                                                                                                                                       >  1:540207/1‑251 (MQ=255)
                             cgcAAGGGCAATTCCATCAAGCGACTAAAGGCAGCTCTGTGCTGTTCATCGAAAGCTGTT‑ACGGCAGCGATTTCCAAGATGT‑GTTCCGCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCTCTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAg                                                                                                                                                                                                                    <  2:486149/251‑1 (MQ=255)
                             cgcAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGAGTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAg                                                                                                                                                                                                                    <  1:282570/251‑1 (MQ=255)
                              gcAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCACAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTTCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGAGCTTCGAAGGCACTGCAATGGTCCGTGCTTTCCGCATTACGGACTTcccgg                                                                                                                                                                                                                   >  1:282866/1‑248 (MQ=255)
                              gcAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAgg                                                                                                                                                                                                                   <  1:85452/251‑1 (MQ=255)
                               cAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACTTGATTTCCGCATTACGGACTTCCAGGa                                                                                                                                                                                                                  <  1:194294/251‑1 (MQ=255)
                                  gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTa                                                                                                                                                                                                               <  1:339824/251‑1 (MQ=255)
                                      aaTTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATc                                                                                                                                                                                                             >  1:268732/1‑249 (MQ=255)
                                       aTTCCAGCAAGCGACTAATGGCAGCTCGTTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATATCAACGATGC‑GTTCCTCGCGCAACTTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGCCATTTAAACCCGCAGCGCGACGGATCACAGGTCGTCACTATCAACCAGGGAAAGCGCTTCGCAGGCGCTGCCATGTTACGAGATTTCCGCATTACGGACTTCCATGATTATc                                                                                                                                                                                                             >  2:521875/1‑248 (MQ=255)
                                         gccatcAAGCGACTAATGGCAGCTCGGTGCTGTTCAGAGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTGCCTCGCGCACATTCGACCAAAAGGTAA‑TGCGCGTCCGTCTGTGGTGGTGTCGGATTCCGTACATTTAACCCAGCTGCGCGACGGCTCCCAGGTGGGCACGCTCAACCAGGGAACGCGCTGCGAAGGCACTGTAATGTTGCGTGATTTCCGCATTTCGTACTTCCAGGAtgttcagtc                                                                                                                                                                                                         <  2:241025/245‑9 (MQ=255)
                                           cAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGTTTTCCGCATTACGGACTTCCAGGATTATCAGGCGac                                                                                                                                                                                                      >  2:116251/1‑250 (MQ=255)
                                               aaGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACCGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCAtt                                                                                                                                                                                                  >  1:484013/1‑251 (MQ=255)
                                                 gCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTgg                                                                                                                                                                                                <  1:113067/251‑1 (MQ=255)
                                                  cGACCAATGGCAGCTCTGTGCTGTTCAACGAAAGC‑GTTGACGGCAGCTAGTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGCACTTCTGTGGTGGTGGCCGATTCCGGACATTTACCCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCTACCAGGGAACGCGCTTCGAAGGCACGGCAATGTTAATTGATGTCCGCATGACGGACTTCTAGGATTATCAGGCTATCACTGGt                                                                                                                                                                                               <  2:364402/251‑1 (MQ=255)
                                                   gtcTAAGGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑GGCACGTCCTTCTGTGGTGGTGGCCGATGCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑c                                                                                                                                                                                             <  2:32233/249‑1 (MQ=255)
                                                    aCTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTGCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTGCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑Ca                                                                                                                                                                                            <  1:244295/251‑1 (MQ=255)
                                                       aaTGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATGTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGAAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCa                                                                                                                                                                                         <  2:477288/251‑1 (MQ=255)
                                                          ggCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGc                                                                                                                                                                                      <  1:102021/251‑1 (MQ=255)
                                                           gCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCg                                                                                                                                                                                     >  1:476880/1‑251 (MQ=255)
                                                                      cTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACAGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCg                                                                                                                                                                           >  1:445570/1‑250 (MQ=255)
                                                                        gTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTgagatcgga                                                                                                                                                                          <  1:556760/249‑9 (MQ=255)
                                                                                aaaGC‑GGTGACGGCAGCGTTTTCAAAGATGT‑GTTCCCCGCGCAAAGTCGACCAAAAGGTAA‑TGCCCGTCCTTCGGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCGGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGAt                                                                                                                                                                <  2:471360/251‑1 (MQ=255)
                                                                                 aaGC‑GGTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATa                                                                                                                                                               <  2:269040/251‑1 (MQ=255)
                                                                                         gACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGAcc                                                                                                                                                         <  1:186361/250‑1 (MQ=255)
                                                                                            ggCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGAt                                                                                                                                                     <  1:509431/251‑1 (MQ=255)
                                                                                               aGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTCTCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGCTACCGCCCAGacggg                                                                                                                                                  >  2:286392/1‑247 (MQ=255)
                                                                                                 cgaattccaaGATGG‑GTTCCTCGCGCAAATTCGCCCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGAAATTTAACCCAGCTGCGCGACGGCTACCAGGTCGTCACTCTCAACCAGGGAACGCGCTTGGAAGGCACTGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGTTTGTCAGGCGATCATTGGA‑CACCAGCCTGATGCGCCCGACCCGACCGCAACCGGCCAGATGGAcc                                                                                                                                                >  2:213928/9‑250 (MQ=255)
                                                                                                 cGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGTTTATCAGGCGGTCATTGGT‑CACCAGTCAGTGGAGCTTGACCCGaaagagcccgtacagatggacc                                                                                                                                                >  2:148787/1‑231 (MQ=255)
                                                                                                     ttCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCCGGGAACGCGCTTCGCAGGCACTGCAATGTTACGTGATTTCCGCATTACGGCCTTCCAGGATTATCAGGCGTTCATTGGT‑CACCAGGCGGGGGCGCTCGACCCGAACGATACCGACCAGATGGACATgcg                                                                                                                                            >  1:306478/1‑251 (MQ=255)
                                                                                                     ttCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCGATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATgc                                                                                                                                             <  1:268079/250‑1 (MQ=255)
                                                                                                     ttCAAAGATGT‑GGTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCAGTGGTGGTGGCCGAGTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCTCATTACGGACTTCCAGGATTATCAGGCGATCAGTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATgc                                                                                                                                             <  1:534710/250‑1 (MQ=255)
                                                                                                      tCAAAGATGT‑GATCCTCGAGCAAATTCGAACAAACGGTAA‑TGCACGTGCTTCTGTGGTGGTGGCCGATTCAGGACATTTAACCCAGCTGCGCGCCGGCTCCCAGGTCGTCACTCTCAACCAGGGAAAGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTACAGGATTATCAGGCGTGCATTGGT‑CACCAGGCGGTGGCGCACGACCCGAACGCTACCGACCAGATGGACATgcac                                                                                                                                           >  2:69358/1‑249 (MQ=255)
                                                                                                           taTGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGAGCTCGACCCGAACGATACCGACCAGATGGACCTGCGCACAtt                                                                                                                                      >  2:148891/2‑251 (MQ=255)
                                                                                                           gATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACAtt                                                                                                                                      <  2:502852/251‑1 (MQ=255)
                                                                                                             tgt‑gTTACTCGCGCAAATTCGACAAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGAAATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATAAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACAAGCGCACATtgt                                                                                                                                    >  2:242232/1‑251 (MQ=255)
                                                                                                               t‑gtTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCTAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑Ca                                                                                                                                                                                            >  1:157561/1‑193 (MQ=255)
                                                                                                                    ccccgcgCAAATTCGACCAAAAGGTAA‑TGCACGTCCGTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCTCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAaca                                                                                                                              <  2:521658/248‑1 (MQ=255)
                                                                                                                      tCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGAGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGTTCATTGGA‑CACCAGGCGGAGGCGCTCGACCCGAACGATACCGACCAAATGGACATGCGcaacttggtgaacac                                                                                                                             >  2:488771/1‑237 (MQ=255)
                                                                                                                        gtgcAAATTCGCCCAAAAGGTAA‑TGCACGTCCGTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCTACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGa                                                                                                                          <  2:43100/249‑1 (MQ=255)
                                                                                                                         cgcAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGac                                                                                                                         >  1:279907/1‑251 (MQ=255)
                                                                                                                          gcAAAGTCGACCAAAAGGTAAGTG‑ACTGCCTTCTGTGGGGGGTGCCGATCCCGGACATGTAACCCAGCTGCGCGACGGCTCCCAGGTTGGCACTCTCAACCAGGGAACGCGCGTCGAAGGCATTGCAATGTTACGTGATTTCCGCATTACGGACTCCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGTCATGCGCACATTGTGGAACACTGaca                                                                                                                        <  2:166811/251‑1 (MQ=255)
                                                                                                                            aaaTTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGTTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACAcc                                                                                                                      >  1:104223/1‑251 (MQ=255)
                                                                                                                              attagACCAAAAGGTAA‑TGCACGGCCTTCGGTGGTGGGGGGCGATTCCGGACATTTAAACGAGCTGCGCGACGGCTCCCAGGTCGTCAAGCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACGTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGa                                                                                                                    <  2:107637/247‑1 (MQ=255)
                                                                                                                              aTTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGCCGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGACCGATACGGCCCCAATGGACATGCGCACATTGTGGATCACTGACACCgg                                                                                                                    >  2:154959/1‑250 (MQ=255)
                                                                                                                                     cAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACCTGCGCACATTGTGGAAAACTGACACCGATCGTGCt                                                                                                             >  2:349522/1‑251 (MQ=255)
                                                                                                                                      aaaaGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTc                                                                                                            <  1:550498/251‑1 (MQ=255)
                                                                                                                                              actGCACCTCCTTCTGTGGTGGGGGCCGATTCCCGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCAGTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGACGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAg                                                                                                      <  2:5825/248‑1 (MQ=255)
                                                                                                                                                 gCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGCAGGCACTGCAATGTTACGTGATCTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGAGCTCGCGCAgaact                                                                                                  >  2:406112/1‑251 (MQ=255)
                                                                                                                                                 gCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAgaact                                                                                                  <  1:379462/251‑1 (MQ=255)
                                                                                                                                                       ccTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCGCCCAGGTCGTCCCTCACAACCAGGGAACGCGCTTCGAAGGCACTGGAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAgaactgaact                                                                                             <  2:431235/250‑1 (MQ=255)
                                                                                                                                                       ccTTCTGTGGGGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGaactgaactg                                                                                            <  2:222233/251‑1 (MQ=255)
                                                                                                                                                             gtggtggtgGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGGTGCGCTCGACCCGAACGATACCAACCAGATGGACATGCGCACATTGTGGAAACCTGACACCGATCGTGCGCGCGCAGAACTGAACTGGGGTAt                                                                                      >  2:473505/1‑251 (MQ=255)
                                                                                                                                                                 gggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCGCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACg                                                                                  <  1:503277/250‑1 (MQ=255)
                                                                                                                                                                   gtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGtt                                                                                >  1:470739/1‑251 (MQ=255)
                                                                                                                                                                     ggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTgg                                                                              >  1:514279/1‑251 (MQ=255)
                                                                                                                                                                        cGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCCCTCTCAAACAGCGCACGCACTTCGCAGGCACTGCACTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGACCGATACCGACCCGCTGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAg                                                                                                      >  1:72058/1‑224 (MQ=255)
                                                                                                                                                                         gATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTAtt                                                                          >  1:304273/1‑251 (MQ=255)
                                                                                                                                                                         gATTCCGGACATCTAACAACGCTGCGCGACTGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTAGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGAATTCCAGGATTATCAGGCGATCAT‑GTTGCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACTAGATGGAAATGCGCACATTGTGTAACACTGACTCCGGTCGTGCTCGCGCAGAACTTAACTGGTGTATCACGTTGGTAtt                                                                          <  2:388783/251‑1 (MQ=255)
                                                                                                                                                                          aTTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGATGGTATTc                                                                         >  1:300066/1‑251 (MQ=255)
                                                                                                                                                                              cGGACCTTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGCCCCGAACGATACCGACCAGATGGACATGCGCACATTGGGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAAATGGTGTATCACGTTGGTATTCACCg                                                                     >  1:363091/1‑251 (MQ=255)
                                                                                                                                                                                gACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCTCCgtg                                                                   <  2:520764/251‑1 (MQ=255)
                                                                                                                                                                                 aCATTTAACCCAGCTGCGAGCCGGCGCCCAGGTCGTCCCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCgtgt                                                                  <  2:347348/251‑1 (MQ=255)
                                                                                                                                                                                   aTTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGCTCATTGGT‑CACCAGGCGGTGGCGCTCGCCCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGCAACCGCTCGTGCTCGCGCAGAACTGAACTGGTGTATCCCGTTGGTATTCACCGTGttt                                                                >  2:520893/1‑251 (MQ=255)
                                                                                                                                                                                      tAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTat                                                              <  2:214145/250‑1 (MQ=255)
                                                                                                                                                                                               tGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGACCTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACtt                                                    >  2:169482/1‑251 (MQ=255)
                                                                                                                                                                                                  gcgACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAac                                                                                                                               >  1:560173/1‑173 (MQ=255)
                                                                                                                                                                                                  gcgACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAac                                                                                                                               <  2:560173/173‑1 (MQ=255)
                                                                                                                                                                                                    gacggtccccAGGGCGTCACTCTCAACCAGGGAGCCCGGTTCGTAGGTACTGCAATGTTACGTGATTTACGCATTACGGACTGCCAGGATTTGCAGGCGATCATTGGT‑CACCAGGCGGGGGCGCTCGACCCGAACGATACCGTGCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGGGTATCACGTTGGTATTCTCCGTGTTTATGATGGCACTTATGGt                                               <  2:306478/244‑1 (MQ=255)
                                                                                                                                                                                                           cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTAc                                          <  1:565228/249‑1 (MQ=255)
                                                                                                                                                                                                                gtcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTg                                     >  2:545258/1‑249 (MQ=255)
                                                                                                                                                                                                                gtcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAg                                   <  1:263898/251‑1 (MQ=255)
                                                                                                                                                                                                                   gtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGt                                >  1:337076/1‑251 (MQ=255)
                                                                                                                                                                                                                     cacgctcAACCAGGGAAGGGGCTTCGAAGGCACGTCATGGTTACGTGATTGCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGG‑CGCCAGGCGGTGGCGCTCGACCCGAACGTTACCGACCAGATGGACATGCGCAGATTGTGGAACACTGGCACCGATGGTGCTCGCGCAGAACGGATCGGGTGTAGCACGTTAGGAGGCACCATGAGTATGATGGCAGTTATGGTCGTACCGCTGAGCGTgg                              <  2:279237/247‑1 (MQ=255)
                                                                                                                                                                                                                       cgctcatccagggacgtcgcTTGGAAGGCCCTGCAATGTTATGTGAGTGCCGCAGTACGGACTTCCAGGACTAGCAGGGGATCAAGGGT‑CACCAGGCGGGGGCGCTCGACCCGAACGATACCGGCCAGATGGACATGCGCACATTGTGGAACACTGTGACCGATCTTGCTAGGGCAGAGCAGAACTGGTGGAGCGCGTTGGTATGGTCCGGTTTTATGATGGCACTTATGGTCGTATCGCTGTGGGTGGtt                            <  2:258993/234‑1 (MQ=255)
                                                                                                                                                                                                                                aGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCGCTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGt                   >  1:333372/1‑251 (MQ=255)
                                                                                                                                                                                                                                 gggAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTc                  >  1:14780/1‑251 (MQ=255)
                                                                                                                                                                                                                                   gAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATGACGGACTTCCAGGATTATCAGGCGAGCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAg                <  2:118784/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCGCGCTTCGAAGGCACTGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAAAGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTAGGAATTCCCCGTGTTTATGATGGCCCTTATGGTCGTCCCGCTGAGCGTGGTTAACCCACGTCAgg               >  2:120171/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cgcgCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAggg              >  1:535071/1‑250 (MQ=255)
                                                                                                                                                                                                                                        cgcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGtt                            <  1:284270/234‑1 (MQ=255)
                                                                                                                                                                                                                                        cgcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGtt                            >  2:284270/1‑234 (MQ=255)
                                                                                                                                                                                                                                         gcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGCTTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGAATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGaagc          >  2:561688/1‑248 (MQ=255)
                                                                                                                                                                                                                                         gcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGGGTTTATGATGGCACTTATGGTAGTACCGCTGAGCGTGGTTAAACCACGTCAGGGCcgc          >  2:306351/1‑251 (MQ=255)
                                                                                                                                                                                                                                            tCGAAGGCACTGCAATGTTTCGTGATTTCCGCATTACGGACTGCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACAAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGAcgcg         <  2:23975/249‑1 (MQ=255)
                                                                                                                                                                                                                                            tCGAAGGAACTGCAATGTTACGTGATTTCCGCATTACGGCCTTCCAGGATTATCAGGCGATCATTGGT‑CCCCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACGTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGAcgcg         <  2:476880/249‑1 (MQ=255)
                                                                                                                                                                                                                                               aaGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTg    >  1:14429/1‑251 (MQ=255)
                                                                                                                                                                                                                                                aGGCACTGCACTGTTACGAGATTTCCGCATTACGGACTTCCAGGATTATCCGGCGCTCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGCTCGTGCACGCGCAGAACTGAACTGGGGTATCACGTTGGAATTCACCGTGTTTGTGATGGCACTTCTGGTCGTACCGCTGAGCGAGGTTAACCCACGTCAGGGCCGCGTACTGt   >  2:173540/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 ggCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTc  >  1:66787/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  
GAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGC‑GTTGACGGCAGCGATTTCAAAGATGT‑GTTCCTCGCGCAAATTCGACCAAAAGGTAA‑TGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGT‑CACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTC  >  NC_000913/4486622‑4487110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑