Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,280	C→T	P75S (CCG→TCG)	ytfR →	galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,280	0	C	T	100.0%	120.9 / NA	46	P75S (CCG→TCG)	ytfR	galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/24);  total (22/24)

GGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAA  >  NC_000913/4451051‑4451513                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ggAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATAAAAGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGACCACgcgc                                                                                                                                                                                                                      >  1:413149/1‑251 (MQ=255)     aCCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCAGGGCGCTGCTCGGTGAAAATGGGGCGGGAAAATCAACGCTAATCAAAGCATTATCTGTTGCATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGcaaca                                                                                                                                                                                                                  <  1:189635/251‑1 (MQ=255)        aTGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGccc                                                                                                                                                                                                                            <  1:289580/238‑1 (MQ=255)        aTGACGACAGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTAAACCACGCCGATCGCGGCCCCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCaggtcggaagagg                                                                                                                                                                                                               >  2:289580/1‑239 (MQ=255)          gacgacCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGGGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCg                                                                                                                                                                                                             >  1:90422/1‑251 (MQ=255)                cgaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGGGTATACAACGCCGATCGCGGCCCCAACAGGCTGGACGGCCCGGCTATATCATCCAAAAATGCCTCCCCCGCGCACCAACTCGGCaatt                                                                                                                                                                                                       >  2:390839/1‑248 (MQ=255)                  accAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGCAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACACCGATCGCGGCCCCCACTGGCTGGCAGGCCCGGCTATCTCATCGAAAAATACACCCCCCGCGCCACCACTCCGCAtttgc                                                                                                                                                                                                     >  2:386601/1‑248 (MQ=255)                       cacCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCCCCGt                                                                                                                                                                                                >  1:303397/1‑251 (MQ=255)                             gagaTCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCCCCATCTGGCTGGAAGGCCAGGCTATCTCCTCGAAAAATACCGCCCACGCCCAACAACTCGGCATTTGCACCGTCTATc                                                                                                                                                                                           >  2:163006/1‑250 (MQ=255)                                 acctacGCACCGAATGATGAATGAAATTTTTCCCCGGCGTCAAAGCGGTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCCCCGTCTAGCAGGaa                                                                                                                                                                                      <  2:96639/246‑1 (MQ=255)                                           cGAAGGATTCAGAAAATTTTTCCCCGGCGTCAAAGCGTTAGCCGACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCAGATCGGTGAAAACGGCGCGGGGAAATCCACGCTCATCAAAGCATTAACTGGTGTATAACAACCCGATCGCGGCACCCTCTGACCGGAAGGCCAGGCTCTCCCCTCGAAAAAAACCGCCCACGCGCAACACATCAGCATTGTCACCGTCTATCCGGAAGTCacacggc                                                                                                                                                                            >  2:290226/1‑245 (MQ=255)                                                  ttAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGGGTATACCACGCCGATCGCGGCACCATCTTGCTGGAAAGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAATGCAACCTGCTACCCa                                                                                                                                                                      >  1:132647/1‑250 (MQ=255)                                                    aatgaaaTTTTGCCCGTGCGTCAAAGCGTTAGGCACAGTTGATCTCAGCATGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCAATAACTGGTGTATACCACGCCGATCGCGGCACCAGCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAata                                                                                                                                                                   <  2:156159/247‑1 (MQ=255)                                                    aaGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCaaa                                                                                                                                                                    <  2:10220/250‑2 (MQ=255)                                                        aaaTTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATgtc                                                                                                                                                               <  1:171203/251‑1 (MQ=255)                                                                  ccGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTTCCCAATATGTCGGTCGCTGAt                                                                                                                                                     <  2:10745/251‑1 (MQ=255)                                                                   cGGCGACAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCAAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATCCCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATa                                                                                                                                                    <  2:25721/251‑1 (MQ=255)                                                                         cAAAGCGTTAGACACCGTTGATGTCAGCCGGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAGCGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCATCAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTa                                                                                                                                               <  2:525064/250‑1 (MQ=255)                                                                            aGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGACCCATCTGGCTGGCATGCCAGGCTATCCCATCGAAAAATACCGCCCACGCGCCACAACTCGGCATTTGCACCGACTATCAGGGCGTCCACCTGCTACCCCATATgccggtaccttataatttatct                                                                                                                                            >  2:30648/1‑234 (MQ=255)                                                                                     cAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCcgcg                                                                                                                                  >  1:431131/1‑251 (MQ=255)                                                                                            gATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCa                                                                                                                            <  1:102444/250‑1 (MQ=255)                                                                                             aTTTCAGCCTGCTCAGTGGCGAAATCATGGCGTTGTTCGGTGAAAACGGGGCAGGAAAATCAACGCTAATCAAAGCATTAACTGGTGGATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAACCTATTTATAGGCCGCGAACCCaaa                                                                                                                          <  1:247055/251‑1 (MQ=255)                                                                                                           cGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGATTCGGCCTTCt                                                                                                            >  1:250854/1‑251 (MQ=255)                                                                                                                 caaaccATGGCGCGTCTCGGTGAAAACGGGGCGGGAAATGCAACGCTTATCAAAGCATTAACGGGTGTATACCTCGCCTATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACGCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCGACCCAATATGTCGGTCGCTTATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGTCTTCTAcgccg                                                                                                      <  2:572306/246‑1 (MQ=255)                                                                                                                            gctgctCGGTGAAAACGGGGCGGGAAAATCCACGCTAATCAAAGCCTTAACTGGTGTATACCACGCCGATCGCGGCCCCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACCACTCGGCATTGGCACCGTCTATCCGGAAGTCCACCTGCTACCCAATATGTCGGTCGCTGATAATATATTTATAAGCCGCGAACCCAAACGCTTCGGCCATCTACTCCTCAAAAAGATgg                                                                                           >  2:422715/1‑251 (MQ=255)                                                                                                                            gcggctCGGTGAAAACGGGGCGGGAAAATCAACGCTAAGCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGGCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATgg                                                                                           <  1:34778/248‑1 (MQ=255)                                                                                                                                          aCGGGGCGGGAAAATCAAAGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCAcc                                                                             <  2:160132/251‑1 (MQ=255)                                                                                                                                                cGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTg                                                                       <  1:341653/251‑1 (MQ=255)                                                                                                                                                       atcctagcgatgctactccttAACTGCGGTATTCCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCCCATCGAAAAATACCGCCCCCGCGCAACAACTCGGCATTGGCACCTTCTATCAGGAAGTCAACCTGCTCCCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAGCGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCAt                                                                <  2:533465/232‑1 (MQ=255)                                                                                                                                                            cGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTAc                                                           >  1:496261/1‑251 (MQ=255)                                                                                                                                                             gCTAATCAAAGCATTAACAGGTGTATACCCCGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACTACGCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACg                                                          <  2:22608/251‑1 (MQ=255)                                                                                                                                                              cTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATATGGCTGGAAGGCCAGGCTATCTCCTCGAAAATTACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGAAAATCTATTTATAGGCCGCGAACCCAAACGCTTAGGCCTTCTACGCCGCAAAGAGATGGAAAAGAGAGCCACCGAACAGATGGAATCTTTCgg                                                         >  2:398691/1‑251 (MQ=255)                                                                                                                                                                aaTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGtt                                                       <  1:89668/251‑1 (MQ=255)                                                                                                                                                                 aTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACg                                                                                                                        >  1:192447/1‑185 (MQ=255)                                                                                                                                                                 aTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACg                                                                                                                        <  2:192447/185‑1 (MQ=255)                                                                                                                                                                 aTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGttt                                                      <  2:496261/251‑1 (MQ=255)                                                                                                                                                                            aaCTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGAc                                            <  1:256941/250‑1 (MQ=255)                                                                                                                                                                                ggTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTgcg                                       <  1:151793/251‑1 (MQ=255)                                                                                                                                                                                       cccACGCCGATCGCGGCCCCAGCTGGCTGGAAGGCCAGGCTATCACATCTAAATATTCCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGTTTTCTCCCTCGACGTGCGCGAAcc                                 <  2:468115/249‑1 (MQ=255)                                                                                                                                                                                        ccACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGc                               >  1:264245/1‑251 (MQ=255)                                                                                                                                                                                             ccGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCCCCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGAAAAGCGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAAc                          >  2:119040/1‑251 (MQ=255)                                                                                                                                                                                             ccGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAACCGCGCCCCCGAACTGCTGGCATCTTCCGGTTTCTCCCTCGACGTGCGCGAACCGCTccac                          >  2:470839/1‑248 (MQ=255)                                                                                                                                                                                                   gcgGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                    >  1:160019/1‑251 (MQ=255)                                                                                                                                                                                                                    gAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCCCCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGCAAAGCGCGCCACCGAACTGATGGCATCTTCCGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATgcagc    >  1:481573/1‑250 (MQ=255)                                                                                                                                                                                                                     aaGGCCAGGCTATCTCATCGAAAACTACCGCCCACGCGCAACAACTCGGCGTTGGCACCGTCTATCAGGAAGTCCACCTGCTACCCAATATGTCGGTAGCTGATAATCTATTTATAAGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAACGAGATGGAAAAGCGCGCCACCGCACTGATGGAATCTTACGGTTTCTCCCTCGACGGGCGCGAACCGCTCAACCGCTTATAAGACGCCATgcagca   >  2:77730/1‑250 (MQ=255)                                                                                                                                                                                                                     aaGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCTCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCaa  <  2:90422/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          GGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAA  >  NC_000913/4451051‑4451513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑