Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,750	C→T	G272G (GGG→GGA)	cpdB ←	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,750	0	C	T	100.0%	220.6 / NA	75	G272G (GGG→GGA)	cpdB	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/38);  total (37/38)

TGCTGACGAACTGGCGTGTGGCATCGTGAT‑CGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAAT  >  NC_000913/4435506‑4435994                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      tGCTGACGAATGGGCGTGTGGCATCG‑GATTCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGTATTTTTTATTAGCGATGTCTTAAATCGGTC‑TGTCTTTCGCTTTCGCCTGCGGCACCGGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCATGGCATTACCGCCGGAACACCATTCAGCGTGCCTCTGGCGATATCAGCTCCTTc                                                                                                                                                                                                                                                  <  2:71581/250‑1 (MQ=255)  gCTGACGAATCGGCGTGTGGCATCTTGAT‑CGGCTTTGAGATTTTCTACCACCTTGCTGTCTTCCGCCTCGAGGGTTTTTTTATTAGCGATGTCGTAAATCGGTCTGG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTTCCTCTTTCATTACTGATTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCTATATCAGCTCCTTCg                                                                                                                                                                                                                                                 <  2:233733/250‑1 (MQ=255)  gCTGACGAACTGGCGTGTGGCATCGTGAT‑CGGCTTTGGGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGGGGGATTTTTTATTAGCGGTGTCGTAAATCGGTCGGG‑CTTCCACTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCCTTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCCTTCAGCGTCCCTTTGGCGATATCAGCTCCTTCgg                                                                                                                                                                                                                                                >  1:444749/1‑250 (MQ=255)             ggCGTGTGGCCTCGTGTT‑CGGCTTTGAGTGTTTCCACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGACGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaaa                                                                                                                                                                                                                                      <  2:219423/250‑1 (MQ=255)             ggCGTGTGGCATCGTGAT‑CGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaaa                                                                                                                                                                                                                                      <  1:107364/250‑1 (MQ=255)                   gtgCATCGTGCT‑CGGCTTTGAGTGTTTCTACCAGCTTTGTGTCTCCCGCCGCGAGGGATTTTTTATTGGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCGGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTa                                                                                                                                                                                                                               <  2:268787/249‑1 (MQ=255)                          gTGAT‑CGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTggg                                                                                                                                                                                                                         >  1:28793/1‑250 (MQ=255)                            gAT‑CGGTTTTGAGTGTTGCTACCAGTTTGTTGCCGTCGGCCGCGAGGGATTTTTTATTAGCGAGGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGAGCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGaaa                                                                                                                                                                                                                      <  2:499028/251‑1 (MQ=255)                            gAT‑CGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGaa                                                                                                                                                                                                                       >  1:389327/1‑250 (MQ=255)                                cGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAAc                                                                                                                                                                                                                    <  1:466484/250‑1 (MQ=255)                                  gCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGc                                                                                                                                                                                                                 <  1:211184/251‑1 (MQ=255)                                        aGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGc                                                                                                                                                                                                           >  1:385673/1‑251 (MQ=255)                                           gtTTCTCCCCGCTTGCTGTCTTCCCCCCCGAGGGATTTTTTATTAGCGAGGTCTTAAAGCGGTGGTG‑CTTCCGCTGTCGCCTGCGTCCCCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGACCGCCCCACATGCCTGGCATTACCTCCGGAACACCATTCAGCGTGCCGTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACTGCGTGAGCATg                                                                                                                                                                                                        <  2:374048/251‑1 (MQ=255)                                              tcttccAGCGTGCTGTCTTCCGCTGCCAGTGATTTTTTTTTAGCGGTTCCGTAACCCGGTCGTG‑CTTCTGCTTCCGCCTGCGTCACCTGCCATTTACCGCTTTCCTTTCTGAGTTTTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTTGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTTGGAAAACGGCGTGCGCATgga                                                                                                                                                                                                      <  2:155480/246‑2 (MQ=255)                                                   cAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑Ac                                                                                                                                                                                                >  1:271309/1‑250 (MQ=255)                                                            gTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGc                                                                                                                                                                                        <  1:368492/249‑1 (MQ=255)                                                            gTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCg                                                                                                                                                                                       <  1:384708/250‑1 (MQ=255)                                                                tCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAAc                                                                                                                                                                                  >  1:41122/1‑251 (MQ=255)                                                                 ccgccgCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTACGCGTGCCTTTAGCGATATCAACTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAACACA‑AATGGCGTTaaac                                                                                                                                                                                 >  2:416285/1‑249 (MQ=255)                                                                     cgcgAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATATTGGCGTTAACGCCCg                                                                                                                                                                             >  1:556423/1‑251 (MQ=255)                                                                      gcgAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGCGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGACAA‑ACATAATGGCGTTAACGCCCgg                                                                                                                                                                            >  1:560916/1‑251 (MQ=255)                                                                          gggATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAAtt                                                                                                                                                                        <  1:21991/251‑1 (MQ=255)                                                                            gATTTTTTGTTAGCGATGTCTTAAATCGTTCTTG‑TTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTTTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCTACATGCCTGGCATTCCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCTCTATCAGCAAAATCTTTa                                                                                                                                                                                                                               <  2:124384/195‑1 (MQ=255)                                                                            gATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTa                                                                                                                                                                                                                               >  1:124384/1‑195 (MQ=255)                                                                                    attaGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAggg                                                                                                                                                              >  1:310587/1‑250 (MQ=255)                                                                                    attaGCGATGTCGTAAATCGGTCGGG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGt                                                                                                                                                              <  1:414975/251‑1 (MQ=255)                                                                                         cGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGCTGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGa                                                                                                                                                                                                                                                >  2:57821/1‑165 (MQ=255)                                                                                         cGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGa                                                                                                                                                                                                                                                <  1:57821/165‑1 (MQ=255)                                                                                         cGATGTCGTAAATCGGTCGTG‑CTTCCACTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGtaata                                                                                                                                                          >  1:493778/1‑250 (MQ=255)                                                                                             gTCGTAACTCGGTCGTG‑CTTCCGTCTTCGCCGGCGGCACCTGCCATTTACCGCTGTCATTACAGAGTTGTAAGTCGACCTCACCAAGATGATCGCCCCACATTCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACt                                                                                                                                                      <  2:63065/250‑1 (MQ=255)                                                                                             gTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATAAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGGGATAATAAACTg                                                                                                                                                     >  2:420542/1‑251 (MQ=255)                                                                                                  aaaTCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGtt                                                                                                                                                 <  1:157981/250‑1 (MQ=255)                                                                                                  aaaTCCGTCGTG‑CTGCCTCTTACGCCTGCGTCACCGGCCATTAACCGTTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGTCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGttt                                                                                                                                                <  2:556423/251‑1 (MQ=255)                                                                                                   aaTCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATaaaagttcgga                                                                                                                                                >  2:404163/1‑242 (MQ=255)                                                                                                   aaTCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATaaa                                                                                                                                                        <  1:404163/242‑1 (MQ=255)                                                                                                          cGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCAt                                                                                                                                        >  1:126033/1‑251 (MQ=255)                                                                                                                   cGCTTTCGCCGGCGTCCCCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCTACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTtat                                                                                                                                <  1:356523/251‑1 (MQ=255)                                                                                                                        tCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACgg                                                                                                                            >  2:324066/1‑250 (MQ=255)                                                                                                                           ccTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCg                                                                                                                        <  1:387470/251‑1 (MQ=255)                                                                                                                            cTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGa                                                                                                                                                                           <  1:448956/199‑1 (MQ=255)                                                                                                                            cTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGa                                                                                                                                                                           >  2:448956/1‑199 (MQ=255)                                                                                                                                  cACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAAAACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGGCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGAt                                                                                                                  >  1:569283/1‑250 (MQ=255)                                                                                                                                              aacgccgcaatgaCTGAGTTGTAAGTCGACGACAACAAACTGCTCGCCCCACATGCCTAGAATTAAAGCCGGAACACCATTCAGCGTGCCTTTGGCGCTATGAGCTACTTCGATAACAGCAAACTCTTTAACTGGGAAAACGGCGTGCGCATGGACAA‑ACATAATGGCGTTAACGCCCGGAGTATCAATGGGGTACTAACCTGAGTTTTCCGACATACCTTTGTACGGATAGGCAGATataccggctgttt                                                                                                     >  2:500437/13‑239 (MQ=255)                                                                                                                                                    gTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCAACACTTTATACGGATCGGCAGATAAGCCGGACTGTGCCAGa                                                                                                >  2:341331/1‑250 (MQ=255)                                                                                                                                                      cATTACTGAGTTGTAAGGCGACCACACCAAGATGAGCGCCCCTCATGCCTGGCATTACCGCCGGAACCCCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAAc                                                                                              <  2:132665/250‑1 (MQ=255)                                                                                                                                                         tACTGAGTTGGAAGTCGACCACACCAAGATGATCGCCCCACATGCCGGGCATTACCGCCGGAACACCATTCAGCGTGCCGTAGGCGATATCAGCTCCTTCGCTATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCAAA‑CCATCATGGCGTTAACGCCAGGATTTTCACTGAGGTGATAAACTGAGTTTTCCGCCCTCCATTTTTACGGATCGGCAGATAGACCGGAATATGCCTGAAcaac                                                                                           >  2:164777/1‑247 (MQ=255)                                                                                                                                                          aCTGAGTTGTAAGTCGACCACACCCAGATGATCGCCCCACATACCGGGCATTACCACCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGGTATCAGCAAAATCTTTACCGGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCAGAAATTTCACTGAAGTAATCAACTGAGTTTTCCGCCATCACTTTATAAGGAACGGCAGATATCCCGAAATGTGCCAGACCGacac                                                                                         >  2:195/1‑250 (MQ=255)                                                                                                                                                            tGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGacaac                                                                                        >  1:562790/1‑250 (MQ=255)                                                                                                                                                             gggTTGAAAGTCGACCCCACCAAGAGTATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGTCCAA‑ACAGAATGGCGTTAACGCCCGGAATTGCACTGAGGTAATAAACTGAGTTGTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGacaac                                                                                        <  2:403551/247‑1 (MQ=255)                                                                                                                                                                     aatCGACCACACCAAGATGATCGACCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGATCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGAGAGCATGGCCAA‑ACATAATGGCGTTAACGACCGGAATTTCACTGAGGAAATAAACTGAGTTTTCGGCCATCACTTTATACGGATCGGCAGATAGCACGGAATGTGCCAGAACGAAAAAAACAGCgga                                                                               >  2:235935/3‑249 (MQ=255)                                                                                                                                                                       tCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGCACACCATTCAGCGTGCCGTAGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGACAA‑ACATAATGGCGTTAAAGCCCGGAATTTCACTGAAGTAATAAACTGAGTTTTCCGCCATCACTTTATAAGGATCGGCAGATAGACAGGAATGTGCAAGAACGACAACAATATAGGCAcc                                                                            >  2:489491/1‑251 (MQ=255)                                                                                                                                                                        cGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGCTATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCCCCt                                                                           >  2:99606/1‑251 (MQ=255)                                                                                                                                                                         gACCACACCCAGAAGATCGCCCCACATGCCTGGCATTACCGCCGGCACACCATTCAGCGTGCCTTTTGCGATATCAGCTCCTTCGATATCCGCAAAATCTTTACCTGGGACAACGGCGTGGGCCTGGCCAA‑ACATACTGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGACCGGCAGATAACCCGGGATGAGACAGaaagccacccatctcggcacctt                                                                          >  2:229202/1‑230 (MQ=255)                                                                                                                                                                                ccAAGATGAGCGCCCCCCTTGCCTGTAATTACCGCCGGAACACCATTCAGCGTGCCTTTTGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAGCGCCCGGAATTTCACTGAGGTAATAAACGGAGTTTTCCGCCCTCACGTTATACGGATCGGCAGATAGCCCGGAATTTGCCAGAATGACAACATCATCGGCACCTTTcgcgcg                                                                   <  2:334620/251‑1 (MQ=255)                                                                                                                                                                                   aGATGATCGCCCCACATGCCTGGCATTCCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGc                                                                                                    <  1:165189/215‑1 (MQ=255)                                                                                                                                                                                   aGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGAGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGCATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTGTATACGGATCTGCAGATAGCCCGGAATGTGc                                                                                                    >  2:165189/1‑215 (MQ=255)                                                                                                                                                                                       gcccGCCCCCCATGCCTGGCATTACCGCCGGAACACCAATCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATAACAGCAAAATCGTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCa                                                            <  2:441877/248‑1 (MQ=255)                                                                                                                                                                                       gatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCa                                                            <  1:300582/251‑1 (MQ=255)                                                                                                                                                                                       gatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑AAATAATGGCGTTAACGCCCGGAATTTCACTGAGGAAATAACATGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGACCGGAATGTGCCAGAACGAACACAACATCGGCACCATTCTCGCGCCTTTc                                                             >  2:305444/1‑250 (MQ=255)                                                                                                                                                                                          cGCCCCGCTTGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCTCTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGc                                                         <  2:450352/251‑1 (MQ=255)                                                                                                                                                                                          cGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGc                                                         <  1:147007/251‑1 (MQ=255)                                                                                                                                                                                            ccccACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCAc                                                       >  2:513017/1‑251 (MQ=255)                                                                                                                                                                                                        ggCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCAc                                           <  1:349837/251‑1 (MQ=255)                                                                                                                                                                                                          cATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACgg                                         >  1:341034/1‑251 (MQ=255)                                                                                                                                                                                                             tACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACAACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGttt                                      >  1:368533/1‑251 (MQ=255)                                                                                                                                                                                                               ccgccgGAACCCCCTTCAGCGTTCTGTTGGCGATACCAGCTCCGTCGATTTCAGCAAAATCATTTCCTGGGAAAACGGCGTGAGCATGGTCAA‑ACATAATGGCGTTAACTCCCGGAATTTCACTGAGGTAATAAACTGAGGTTTCCGCCATCACTTTATTCGGCTCGGCAGATAGCTCGGAATGTGCCAGGACGACAACAACATCGGCACCTTTCTCTCGCATTTCGTGCACGTAGTTGCGCTCGGGTTCg                                    <  2:325323/251‑1 (MQ=255)                                                                                                                                                                                                                 gccgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCGTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTGCTCGCGCATTTCAGGCACGTATGTGCGCACGGTTTCGGt                                  <  1:506741/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAatat                             <  1:324066/251‑1 (MQ=255)                                                                                                                                                                                                                         acCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCAt                          >  2:416867/1‑251 (MQ=255)                                                                                                                                                                                                                             ttCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCAc                      >  1:289070/1‑251 (MQ=255)                                                                                                                                                                                                                                     gCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAAGCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACt               <  1:537360/250‑1 (MQ=255)                                                                                                                                                                                                                                      ccTTTGGCGATTGTAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTTAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTAGTTGCGCACGGTTTCGGTAATATCATTCACCGTCACttt             <  2:271309/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCg         <  1:341331/250‑1 (MQ=255)                                                                                                                                                                                                                                               atatCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAATGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATa     >  1:194770/1‑250 (MQ=255)                                                                                                                                                                                                                                                 atCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCCCTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGCTaact  >  2:234170/1‑249 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TGCTGACGAACTGGCGTGTGGCATCGTGAT‑CGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTG‑CTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAA‑ACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAAT  >  NC_000913/4435506‑4435994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑