Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,426,929 C→T C165Y (TGC→TAC)  yjfZ ← DUF2686 domain‑containing protein YjfZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,426,9290CT97.4% 117.0 / NA 39C165Y (TGC→TAC) yjfZDUF2686 domain‑containing protein YjfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/19);  minor base A (1/0);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCA  >  NC_000913/4426683‑4427165
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
tttGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCTAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAAtt                                                                                                                                                                                                                                          >  1:456499/1‑251 (MQ=255)
      ggCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCAt                                                                                                                                                                                                                                    >  1:104986/1‑251 (MQ=255)
                 catacGAGCGCAACTGATGTCTCGCGCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGaa                                                                                                                                                                                                                         <  2:104986/251‑1 (MQ=255)
                      gAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGtt                                                                                                                                                                                                                    <  2:294154/251‑1 (MQ=255)
                      gAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGtt                                                                                                                                                                                                                    <  1:75589/251‑1 (MQ=255)
                      gAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATAATGGGGTAAATCAGAATACGCGTTTAGCAAGGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGCTGACCAGTAATTAGGCATTTGGGCAGGAAGGGtt                                                                                                                                                                                                                    >  2:172341/1‑251 (MQ=255)
                       aGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGTTGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGACGTTAGTCGATATAAAGTTCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGtt                                                                                                                                                                                                                    >  2:569151/1‑250 (MQ=255)
                        gcgcAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAACCCCGAGTAGATTGCGGCTGCACTTGATAAGGCACGCAGACCAATTTTCAAAACATGGGGTAAATCAGAATACGCGTTTCGCAATGCAGCAATGATGAATACGAGGGCATTAGAAACGACTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGAACAGAAATGAGGCATTTGGGCAGGAAGGGTTAc                                                                                                                                                                                                                  >  2:559534/1‑251 (MQ=255)
                        gcgcAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTAc                                                                                                                                                                                                                  <  1:275162/251‑1 (MQ=255)
                           cAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAAc                                                                                                                                                                                                               >  1:511766/1‑251 (MQ=255)
                                           ccTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGCACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTgg                                                                                                                                                                                               >  2:385981/1‑251 (MQ=255)
                                                        ctcgAAAACCAAACCCGAGTAGAATGCGGCTGAACTTGGTAAGGCACGCAGACCACTTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAGTCCGAGGGCATTAGAAACGCCTGTAACAGTGCGACGTTGGTCGATATAAAGATCGATTGTTGGTAGTTAAGATGACCCGTAATTAGGCATTTGGGCTGGACGGGTTACAACGTATCCTATCGTTTGGCCGGTATCGTCTg                                                                                                                                                                                  >  2:527121/1‑251 (MQ=255)
                                                                       cGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAcc                                                                                                                                                                     >  1:119850/1‑249 (MQ=255)
                                                                         aGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGACGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTg                                                                                                                                                                                                >  2:158539/1‑220 (MQ=255)
                                                                         aGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTg                                                                                                                                                                                                <  1:158539/220‑1 (MQ=255)
                                                                                     cTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACt                                                                                                                                                     >  1:565047/1‑251 (MQ=255)
                                                                                         aCTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCgt                                                                                                                                                 <  1:285829/251‑1 (MQ=255)
                                                                                             gATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAg                                                                                                                                                                   >  2:349537/1‑229 (MQ=255)
                                                                                             gATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAg                                                                                                                                                                   <  1:349537/229‑1 (MQ=255)
                                                                                                                   tCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCg                                                                                                                       >  1:474710/1‑251 (MQ=255)
                                                                                                                         cATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGAt                                                                                                                 <  1:221083/251‑1 (MQ=255)
                                                                                                                            ggggTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTAGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCg                                                                                                              <  1:569151/251‑1 (MQ=255)
                                                                                                                               gTAAATCAGAATACGCGTTTAGCAATGCAGAAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACATTGCGAAGTTGGTTGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGGGTATTTACTGTTGGGTTCGAAGGATTCGACa                                                                                                           <  2:511766/251‑1 (MQ=255)
                                                                                                                                gaaaTCAGAATACGCGTGTAGAAATGTAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGATGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAATACCACGTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACAt                                                                                                          <  2:342919/250‑1 (MQ=255)
                                                                                                                                                           agcaATGATGCATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACAGTAAACAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGACGGATTCACCATCTTGCAATAAATATTTCATTTATGCgg                                                                               >  2:419443/1‑251 (MQ=255)
                                                                                                                                                            gcaATGATGAATCCGAGGGTATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATGTCAGTTCTGCGGt                                                                              <  2:474710/251‑1 (MQ=255)
                                                                                                                                                             caATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGtt                                                                             <  1:385981/251‑1 (MQ=255)
                                                                                                                                                                            gggCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCcgcg                                                              >  1:451764/1‑251 (MQ=255)
                                                                                                                                                                                   aGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCt                                                        <  1:47220/250‑1 (MQ=255)
                                                                                                                                                                                                 cAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTgg                                         >  1:419111/1‑251 (MQ=255)
                                                                                                                                                                                                     gCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGGTGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTg                                                                                  >  2:12980/1‑206 (MQ=255)
                                                                                                                                                                                                     gCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTg                                                                                  <  1:12980/206‑1 (MQ=255)
                                                                                                                                                                                                     gCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCg                                      <  1:527121/250‑1 (MQ=255)
                                                                                                                                                                                                        aaGTTGGTCGATAGAAAGATCGATTGATGGTAGTGCAGATGACCTGTAATTAGGCATTTGGGCAGGAAGTATTACAACGGATCCTATCGTTTGGCCGGTATAGTCGGGAGTACCATAACCAGATCACAAAACCACTTCGGGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTTCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGa                                   >  2:379333/1‑250 (MQ=255)
                                                                                                                                                                                                                cGATATAAAGATCGATTGTTGGTAGATCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTCTTGTATTTCTGGGCGTGAATATTATCt                          >  2:388156/1‑251 (MQ=255)
                                                                                                                                                                                                                  atatAAAGATCGATTGTTGGTAGTTCAGCTGACCAGTAATTAGGCATTTGGACAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACAAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTATTGGGTTCGAAGGATTCGACCTCTTGCAATAACTATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGAGAATATTATCTa                         >  2:272915/1‑250 (MQ=255)
                                                                                                                                                                                                                                   tgGTAGTTCAGATGACCAGTAAGTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtc        <  2:173757/250‑1 (MQ=255)
                                                                                                                                                                                                                                     gTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtccat     <  1:379333/251‑1 (MQ=255)
                                                                                                                                                                                                                                        gTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtccatcca  <  1:272915/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
TTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATCCA  >  NC_000913/4426683‑4427165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑