Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,407,045	C→T	A131V (GCT→GTT)	rnr →	RNase R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,407,045	0	C	T	97.4%	238.7 / NA	77	A131V (GCT→GTT)	rnr	RNase R
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (33/41);  minor base G (1/1);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGCTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCGGTGGGT  >  NC_000913/4406801‑4407277                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gagCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCAACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGAGCTGTCTCACCCGCTGGGGGTTGACCg                                                                                                                                                                                                                                    >  2:379369/1‑251 (MQ=255)     agcTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGCCCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGTCTACGGCTTTCTTCGGGTTGCATGGCGTAAAGACG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCgcaa                                                                                                                                                                                                                                 >  1:316316/1‑247 (MQ=255)         tGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAgg                                                                                                                                                                                                                              <  1:344680/249‑1 (MQ=255)             ggCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGtcgtcg                                                                                                                                                                                                                        <  1:169619/251‑1 (MQ=255)              gCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGtcgtcgg                                                                                                                                                                                                                       >  1:255792/1‑250 (MQ=255)                        ccgccTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGt                                                                                                                                                                                                              >  1:159780/1‑250 (MQ=255)                              gcgcgcGATGGAGCGCGATGGTCAACTGGTCTTCACTCGGCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTc                                                                                                                                                                                                        <  2:87997/250‑1 (MQ=255)                                gcgcgATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCGCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCcgc                                                                                                                                                                                                     <  1:482288/251‑1 (MQ=255)                                             cgATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCa                                                                                                                                                                                         >  1:364396/1‑250 (MQ=255)                                              gATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGAc                                                                                                                                                                                                                                      <  1:227596/204‑1 (MQ=255)                                              gATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGATGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCGCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGAc                                                                                                                                                                                                                                      >  2:227596/1‑204 (MQ=255)                                               aTGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTTTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCaaaa                                                                                                                                                                                      <  1:379369/251‑1 (MQ=255)                                                tGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGCGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGCCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCaaaa                                                                                                                                                                                      >  2:568604/1‑250 (MQ=255)                                                 ggTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCaaaaa                                                                                                                                                                                     <  1:131869/250‑1 (MQ=255)                                                     aaCTGGTCTTCAATCGTCGTCAGTGCTATCCGCTGCCGGAACGACTAGACCTGGTGAAAGGTACCGTCATTGGCCACCGTGATGGCCACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGAATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGTTCAGGTGCTGGCTCAGCCGCTGGGCGTTGACCGTACAGGTCGTCGTGACGCGCGGATTGTCCGCGTACTGGTGCCAAAAAccagc                                                                                                                                                                                >  2:178770/1‑251 (MQ=255)                                                         ggTCTTCCCTCTTCGTCATTGCTATGCGCTGCCTGAACGCCTCGACCTGGTGAAAGGTTCCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGTGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAAccagcca                                                                                                                                                                              <  2:406045/249‑1 (MQ=255)                                                         ggTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGa                                                                                                                                                                            <  1:144891/251‑1 (MQ=255)                                                                      cgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTCTTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCCGCCCGATTGATGGCCGCtc                                                                                                                                                               >  1:278762/1‑250 (MQ=255)                                                                      cgtcAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTa                                                                                                                                                               <  1:59256/251‑1 (MQ=255)                                                                         cAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGCTCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTcgtcgt                                                                                                                                                                                                                       >  2:213704/1‑192 (MQ=255)                                                                         cAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTcgtcgt                                                                                                                                                                                                                       <  1:213704/192‑1 (MQ=255)                                                                          aGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACttt                                                                                                                                                           <  1:71328/251‑1 (MQ=255)                                                                                       gCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCTGGGTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAATGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGGCGCTACTTTACCGAAGCGGGc                                                                                                                                               <  2:26905/250‑1 (MQ=255)                                                                                             actccgcGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTTCGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAACCCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCt                                                                                                                                        <  2:408087/245‑1 (MQ=255)                                                                                                ccTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTtg                                                                                                                                     >  1:70462/1‑251 (MQ=255)                                                                                                       cTGGTGAAAGGTACCGTTATTGGCCACAGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTcc                                                                                                                              >  1:230549/1‑251 (MQ=255)                                                                                                         ggTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCt                                                                                                                             <  1:345819/250‑1 (MQ=255)                                                                                                         ggTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTg                                                                                                                            <  1:212314/251‑1 (MQ=255)                                                                                                            gAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTgacg                                                                                                                         >  1:559476/1‑251 (MQ=255)                                                                                                              aaGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTgacgcc                                                                                                                       >  1:239344/1‑249 (MQ=255)                                                                                                               aGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCGGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCGGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCTTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGaa                                                                                                                                                     <  1:515741/220‑1 (MQ=255)                                                                                                                    ccGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCgc                                                                                                                 >  1:268587/1‑250 (MQ=255)                                                                                                                       ttATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCt                                                                                                               <  2:159780/250‑1 (MQ=255)                                                                                                                       ttATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTg                                                                                                              >  1:280910/1‑251 (MQ=255)                                                                                                                         aTTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGCAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTg                                                                                                              >  1:21509/1‑249 (MQ=255)                                                                                                                         aTTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGa                                                                                                             <  1:157830/250‑1 (MQ=255)                                                                                                                                aCCGTGCTGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGAATCTCTCCAGCGAGCAGATGAGAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTCAAGGTCATCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGGTTGTTGGTCGATACTTTGCCGAAGCGGGCGTCGGATTTATGGTTCCTgacgcaagctgtatgaacgtcgct                                                                                                     >  2:330501/1‑231 (MQ=255)                                                                                                                                 ccGTGATGGCTACGGCTGTCTGCGGGTGGAAGGGAGTAAAGATG‑ATTTGTACCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCTATCAGGGGCTGGATCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAACCCAGCCAGATTGTTGTTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGata                                                                                                    <  2:232098/251‑1 (MQ=255)                                                                                                                                     gttGGCTACGGCTTGCTGCGGGTTGAAGGGCGTAAAGAGG‑ATTTGTATCTGTCCAGCGAGCATATGAAAACCTGCATTCTTGGCGAGCAGGTGCTGGCTCAGCCGCTGGGTGTTGCCCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTTGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCTTTTGAGCTTCGATATCtt                                                                                                <  2:46660/249‑1 (MQ=255)                                                                                                                                     gATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCTAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGGTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCtt                                                                                                >  2:425674/1‑251 (MQ=255)                                                                                                                                     gATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCtt                                                                                                <  1:45625/251‑1 (MQ=255)                                                                                                                                     gATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCtt                                                                                                <  1:544121/251‑1 (MQ=255)                                                                                                                                          cTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCGCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGAGTTGACCGAAAAGGTCGTCGTGAAGCGAGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGATACTTTACCGAAGCGGTCGTCGGCTTTGTGGTTCCTGAGGACCACCGTCTGAGCTTCGATATCTTAATcc                                                                                           >  2:184214/1‑251 (MQ=255)                                                                                                                                            aCGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATccc                                                                                          <  1:256521/250‑1 (MQ=255)                                                                                                                                              ggCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATcccgcc                                                                                       <  1:90876/251‑1 (MQ=255)                                                                                                                                                    cTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTgg                                                                                                                                                                                              <  1:237206/142‑1 (MQ=255)                                                                                                                                                    cTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTgg                                                                                                                                                                                              >  2:237206/1‑142 (MQ=255)                                                                                                                                                         ggTTGAAGGGCGTAAAG‑TGTAGTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCTCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCgatcagatca                                                                            <  2:341255/251‑1 (MQ=255)                                                                                                                                                           ttGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGAGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGAGCGTCTTGTCCGCGTACTGGTGCCAAAAACCAGCAAGATTGTTGATCGCTAATTTAACGAAGCGGGCGTCGGATTTGTGGCTCCTGACGACCGCCGTACGAGCTTCGATGTCTTCATcctgccacattaggtcatg                                                                          >  2:216441/1‑238 (MQ=255)                                                                                                                                                            tGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATgg                                                                         >  1:171171/1‑251 (MQ=255)                                                                                                                                                                 ggCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGgcgcg                                                                    >  1:37304/1‑251 (MQ=255)                                                                                                                                                                   cGTAAAGATG‑ATGTGTATCTCTCCAGAGAGCAAGTGAAAACCTGCATTCATAGAGATCAGGAACTGGATCAGCAGCTGGGAAATGACCGTAAAGGACATCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGGTGGGTGCTAATTTACCGAAACGGACGTCGACTATGTGGATCCTGGCGACCGCACTCTGAGCTTCGATATCTTACTCCCGCCAGCTCAGAACATGggcgggcg                                                                  >  2:377043/1‑251 (MQ=255)                                                                                                                                                                      aaaGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCGAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGAt                                                               <  1:164690/251‑1 (MQ=255)                                                                                                                                                                         gatg‑atTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATggg                                                            <  1:22528/251‑1 (MQ=255)                                                                                                                                                                               tttGTAGCCCTCCAGCGATCCGATGAAAACCTGCATTCATGGCGAGCAGGTGTTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGCTTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAAGCCCGCCCGATCAGATCATTGGCGCGCGGATGGGCttt                                                        <  2:85947/250‑1 (MQ=255)                                                                                                                                                                                ttGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTtg                                                       <  1:568604/250‑1 (MQ=255)                                                                                                                                                                                    aTCTCTCTAGCGAGCAGATGAAAACCTGCATTCATGGCGTTCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATGGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGTCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGt                                                   <  2:171171/250‑1 (MQ=255)                                                                                                                                                                                     tctctcCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCg                                                 >  1:410950/1‑251 (MQ=255)                                                                                                                                                                                         tcCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCCGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGt                                             >  1:311154/1‑251 (MQ=255)                                                                                                                                                                                         tacagcgggaaGATGAAAAACGGCATTCATGGCAATAAAGTACTGGATCAGCCGCTGGGAGTTGACCGTGAAGGTAGTCGGGAAGCGGGTATTGTCAGCGTACAGGAGCAACACACCAGCACGCTTGTTGGTCGCTACTTTACCGAAGCGGGCGTAGGATTTGCAGTTCCTGATGAACGCAGTCTGAGCTTCGATATCGAAATCCCGCCCGATCAGATCATGGGCACGGGGAGAGGCTTTTGGGTCGTAgg                                             >  2:280746/11‑250 (MQ=255)                                                                                                                                                                                            aGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGCGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCg                                           >  1:278252/1‑250 (MQ=255)                                                                                                                                                                                               gAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACt                                       >  1:178881/1‑251 (MQ=255)                                                                                                                                                                                                  aaGATGAAAATCTGGATCCAGGGCGATCAGTTGCGTGCGCAGCCGCTGGGTGTTGACCGTATAGGTCTTCGTGAAGCGCGTATTGGCCGCGGTCTGGTGCCAAAACCCAGCCAGTTTGTTGGTCGCTACCTTACCGCAGTGGGCGTCGGCTTGGTGGTTCCTGACGTCAGCCGTCTGAGCTTGGATTTCTTAAGCCCGCTCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCTAACTgtc                                    <  2:433029/250‑3 (MQ=255)                                                                                                                                                                                                     aTGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGTTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGc                                                           <  2:148567/225‑1 (MQ=255)                                                                                                                                                                                                     aTGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGc                                                           >  1:148567/1‑225 (MQ=255)                                                                                                                                                                                                       gaataCCTGTATTTATGGCGATCAGGGGCTGGATCAGCCGCTGGGGGTTGACCGTAAAGGGCGTCGTGAAGCGCGTATTGTCCGCGGACTGGTGCCAAAATCCAGCCAGATTGTGGGGCGCTACTGTACCGAAGAGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAg                                <  2:430746/246‑1 (MQ=255)                                                                                                                                                                                                             gctccttcagggagaccgggTGCTGGCTCATCCGGTGGGTGTTGACCGTAAAGGTCGGCGTGAAGCTCGTATTGTCCGCGTACGGGTGCCATAAACTAGCGAGATTGTTGGTCGCTGCGTTTCCGAAGCGGGCGTCGGTTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTGAATCCCGCCCGATCAGATCATGGGCGCGCGGAGGGGCTTTGTGGTCGTAGTCGAACTGACTTAGCGTCCg                          <  2:311154/232‑1 (MQ=255)                                                                                                                                                                                                             ctacgttCAGGGCAATCAGGTGCTGGCTCAGCCGCTGGGGGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTACCAAAAACCAGGCAGATTGTTGGGCACTACTTTAACAAAGCGGACCTCGGCTTTGTGGTTCCTGACAACAGCCGGCCGACCTTCTATTTCTGGCTCCCGGCCGATCCGATAAGGGACGGGCGGATGGAATTTGTGGTCGTAGTCAAACTgcccgagagtcat                          >  2:204234/6‑238 (MQ=255)                                                                                                                                                                                                               gCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACt                       <  1:409907/251‑1 (MQ=255)                                                                                                                                                                                                                    cATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTg                                                                                                                            <  2:452709/145‑1 (MQ=255)                                                                                                                                                                                                                    cATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTg                                                                                                                            >  1:452709/1‑145 (MQ=255)                                                                                                                                                                                                                       ggCGATCAGGTGCTGGCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGTATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCAc               <  1:166713/251‑1 (MQ=255)                                                                                                                                                                                                                             cAGGTGCTGGCTCAACCGCTGGGTGTTGACAGTAACGGTCGTCATGAAGCGCGTATCGTCCGCGTACTGGTGCCAGAAACCAGACAGATTGTTGGGCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGGTCCTGACGCCAACCGTCTGGGCTTGGATATCTTAATCCCGCCCCATCCGCTCATGGCAGCGCTGATGGGCTTTTGGGTCGTAGACGAACTGACTCAGCGGCCGACccgccgacacaa            >  2:501399/1‑242 (MQ=255)                                                                                                                                                                                                                              aGGTGCTGGGTCAGCCGCTGGGTGTTGACCGTAAAGGGCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCGTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCg        <  1:80238/251‑1 (MQ=255)                                                                                                                                                                                                                                  gCTGGCTCAGCCGCTGGGTGGTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCGGTgg    <  1:152108/251‑1 (MQ=255)                                                                                                                                                                                                                                     ggCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCGGTggg   <  1:425674/249‑1 (MQ=255)                                                                                                                                                                                                                                     ggCTCAGCCGCTGGGTGTTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCGGTGGGt  >  1:281004/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATG‑ATTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCTCAGCCGCTGGGTGCTGACCGTAAAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCGGGCGTCGGCTTTGTGGTTCCTGACGACAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCGGATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCGGTGGGT  >  NC_000913/4406801‑4407277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑